Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Gonadal Sex Differentiation of Hatchery-Reared Longtooth Grouper (Epinephelus bruneus)

  • Sao, Pham Ngoc (Marine and Environmental Research Institute, Jeju National University) ;
  • Hur, Sang-Woo (Marine and Environmental Research Institute, Jeju National University) ;
  • Lee, Chi-Hoon (Marine and Environmental Research Institute, Jeju National University) ;
  • Lee, Young-Don (Marine and Environmental Research Institute, Jeju National University)
  • Received : 2012.08.09
  • Accepted : 2012.09.08
  • Published : 2012.09.30
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Abstract

For the gonadal sex management of younger longtooth grouper (Epinephelus bruneus), this work investigated the timing and histological process of ovary differentiation and oocyte development of longtooth grouper larvae and juvenile. Specimens (from 1 to 365 DAH) were collected for gonadal histological study from June 2008 to August 2009. Rearing water temperature was ranged from 20 to $24^{\circ}C$. The primordial germ cells could be observed from 10 to 15 DAH, while undifferentiated gonad occurs from 20 to 50 DAH in longtooth grouper. The initial ovarian phase was 60 to 110 DAH with the formation of ovarian cavity and the increased in size of gonad. The ovarian phase started at 140 DAH with appearance of oogonia. The gonad at 365 DAH appeared to have full of oogonia and primary growth stage oocyte. Formation of ovarian cavity indicates that the ovarian differentiation beginning at 60 DAH in longtooth grouper. The gonads in longtooth grouper differentiated directly into ovaries in all fish examined.

Keywords

References

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