YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Inhibitory Effects of Purified Bee Venom on Melanin Synthesis

정제봉독의 멜라닌 생성 억제 효과

  • Han, Sang-Mi (Department of Agricultural Biology, National Academy and Agriculturual Science) ;
  • Kim, Jung-Min (Department of Agricultural Biology, National Academy and Agriculturual Science) ;
  • Lee, Kyung-Gill (Department of Agricultural Biology, National Academy and Agriculturual Science) ;
  • Park, Kwan-Kyu (Catholic University of Daegu School of Medicine) ;
  • Chang, Young-Chae (Catholic University of Daegu School of Medicine)
  • 한상미 (국립농업과학원 농업생물부) ;
  • 김정민 (국립농업과학원 농업생물부) ;
  • 이광길 (국립농업과학원 농업생물부) ;
  • 박관규 (대구가톨릭대학교 의과대학) ;
  • 장영채 (대구가톨릭대학교 의과대학)
  • Received : 2012.06.06
  • Accepted : 2012.08.01
  • Published : 2012.08.31
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Abstract

To further access honeybee (Apis mellifera L.) venom (BV) as a cosmetic ingredient and potential external treatment for topical use, we investigated its ability to inhibit tyrosinase activity and melanin biosynthesis on melanogenesis in B16F1 melanoma cells. We found that BV increased the cell viability in B16F1 melanoma cell and BV (0.01~1 ${\mu}g/ml$) inhibited melanin synthesis in with 10 nM ${\alpha}$-melanocyte-stimulating hormone (${\alpha}$-MSH) for 48 h. In addition, we used reverse transcription-polymerase chain reaction and western blotting for me melanogenesis-related genes such as tyrosinase to examine the mechanisms underlying the inhibitory effects of BV on melanogensis. BV inhibited direct tyrosinase activity, which decreased melanin synthesis in ${\alpha}$-MSH stimulated B16F1 melanoma cells. Thease findings suggest that BV induces the downregulation of melanogenesis by inhibiting tyrosinase activation.

Keywords

References

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