Korean Journal of Veterinary Research (대한수의학회지)
- Volume 52 Issue 3
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- Pages.169-176
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- 2012
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
DOI QR Code
Isolation, characterization, and evaluation of Bacillus thuringiensis isolated from cow milk
- Kweon, Chang-Hee (Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA)) ;
- Choi, Sang-Yoon (School of Pharmacy, SungKyunKwan University) ;
- Kwon, Hyog-Young (School of Pharmacy, SungKyunKwan University) ;
- Kim, Eun-Hye (School of Pharmacy, SungKyunKwan University) ;
- Kang, Hyun-Mi (Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA)) ;
- Moon, Jin-San (Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA)) ;
- Jang, Geum-Chag (Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA)) ;
- Lee, Hee-Soo (Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA)) ;
- Kang, Seung-Won (Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA)) ;
- Kim, Jong-Man (Animal, Plant and Fisheries Quarantine and Inspection Agency (QIA)) ;
- Pyo, Suhkneung (School of Pharmacy, SungKyunKwan University) ;
- Rhee, Dong-Kwon (School of Pharmacy, SungKyunKwan University)
- Received : 2012.04.19
- Accepted : 2012.08.14
- Published : 2012.09.30
Abstract
Probiotics colonize the intestines and exert an antibacterial effect on pathogens. Therefore, probiotics could be used as a preventive agent against lethal infections. To isolate probiotic microorganisms, 116 bacterial strains were isolated from healthy cow's milk and were subjected to Gram-stain, morphology and biochemical analyses, Vitek analysis, and 16S rRNA analysis. One of the strains identified as Bacillus (B.) thuringiensis 87 was found to grow very well at pH 4.0~7.0 and to be resistant to high concentrations of bile salts (0.3~0.9% w/v). B. thuringiensis was susceptible to the antibiotics used in the treatment of bovine mastitis, yet it exhibited an antimicrobial effect against Staphylococcus (S.) aureus 305. Moreover, it protected mice from experimental lethal infections of E. coli O55, Salmonella typhimurium 01D, and S. aureus 305 through a significant induction of interferon-