대한수의학회지 (Korean Journal of Veterinary Research)
- 제52권2호
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- Pages.125-131
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- 2012
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
DOI QR Code
새로운 국내 분리 토끼출혈병바이러스(RHDVa)를 감염시킨 토끼에서의 경시적인 병리학적 변화와 조직 내 바이러스 항원 분포
Sequential pathologic changes and viral distribution in rabbits experimentally infected with new Korean strain of rabbit hemorrhagic disease virus (RHDVa)
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박중원
(농림수산검역검사본부) ;
- 전지은 (농림수산검역검사본부) ;
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양동군
(농림수산검역검사본부) ;
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박은정
(연세대학교 치과대학) ;
- 김한 (농림수산검역검사본부) ;
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이명헌
(농림수산검역검사본부) ;
- 황의경 (상지대학교 동물자원학과) ;
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이중복
(건국대학교 수의학과) ;
- 우계형 (세명대학교 임상병리학과)
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Park, Jung-Won
(Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
- Chun, Ji-Eun (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
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Yang, Dong-Kun
(Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
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Bak, Eun-Jung
(Oral Cancer Research Institute, College of Dentistry, Yonsei University) ;
- Kim, Han (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
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Lee, Myeong-Heon
(Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
- Hwang, Eui-Kyung (Department of Animal Science, College of Life Science and Natural Resources, Sangji University) ;
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Lee, Chung-Bok
(Department College of Veterinary Medicine, Konkuk University) ;
- Woo, Gye-Hyeong (Department of Clinical Laboratory Science, Semyung University)
- 투고 : 2012.05.11
- 심사 : 2012.06.21
- 발행 : 2012.06.30
초록
Rabbit hemorrhagic disease is a highly acute and fatal viral disease caused by rabbit hemorrhagic disease virus (RHDV). Since first outbreak in Korea 1987, RHDV has been continually affected in the country, but the pattern of outbreak seem to be changed. In this study, to understand the pathogenesis of the new RHDVa serotype, we therefore carried out to inoculate RHDVa to rabbits, and to examine the sequential histopathologic changes and viral distribution. Macroscopically, various sized dark red or white spots or appearance were observed in the liver, lung, kidney uterus and ureter. In euhanized rabbits, significant pathologic findings such as infiltration of heterophils and mononuclear cells were observed at 24 hours after inoculation (HAI), and these were sequentially extended periportal to centrilobular area. However, in dead rabbits, severe hepatic degeneration and/or necrosis with relatively weak inflammatory responses were observed. RHDV antigens began to detect in liver, spleen, and lung from 12 HAI by PCR. Immunohistochemically, RHDV positive cells were seen in only liver from 24 HAI, and the degree of immunogen reactivity was stronger in dead rabbits than in euthanized ones. In conclusion, RHDVa caused the subacute or chronic infection accompanying low mortality and moderate to severe inflammatory reaction in rabbits, suggesting the possibility that RHD could become endemic.
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