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Anti-inflammatory Effects of Water Extract of Eucommia ulmoides OLIVER on the LPS-induced RAW 264.7 Cells

대식세포 Raw 264.7에서 두충의 항염증효과

  • Kim, Young-Ock (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Sang-Won (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Sohn, Sang-Hyun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Seung-Yu (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Oh, Myung-Sook (Department of Oriental Pharmaceutical Science, College of Pharmacy Kyung Hee University) ;
  • Kim, Su-Kang (Kohwang Medical Research Institute, College of Medicine, Kyung Hee University)
  • 김영옥 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이상원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 손상현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김승유 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 오명숙 (경희대학교 약학대학 한약학과) ;
  • 김수강 (경희대학교 의과대학 고항의학연구소)
  • Received : 2012.04.07
  • Accepted : 2012.10.05
  • Published : 2012.10.30

Abstract

Eucommia ulmoides OLIVER (EU) is a traditional Korean herbal used for the treatment of rheumatoid arthritis (RA). In the present study, the molecular pharmacology basis of its anti-inflammatory effect is revealed in this work, EU was studied in lipopolysaccharide (LPS)-activated macrophage cells (RAW 264.7) as an established inflammation model. After activation, nitric oxide (NO) production and iNOS mRNA were measured by using a colorimetric assay (Griess reagent), and reverse transcription polymerase chain reaction (RT-PCR), respectively. The change in the content of $PGE_2$, $TNF{\alpha}$, and IL-6 was concurrently monitored by ELISA. In results, we found that in the concentration range without showing cytotoxicity, EU produced a remarkable anti-inflammatory effect and showed a dose-dependent inhibition of LPSinduced NO production. Compared with indomethacin, EU has more potency and a specific action of NO inhibition, $PGE_2$, IL-6, and TNF-${\alpha}$ inhibition. These results suggest that EU may be a suitable herbal medicine to yield the greatest anti-inflammatory activity for food additives and medicine.

Keywords

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