Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Variation of Bolting at Cultivation of Different Regions and Molecular Characterization of FLC homologs in Angelica gigas Nakai

재배 지대에 따른 참당귀의 추대 변이와 FLC 유전자 특성

  • 김영국 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 여준환 (전라남도한방산업진흥원) ;
  • 안태진 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 한신희 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 안영섭 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박충범 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 장윤희 (고려대학교 생명과학대학) ;
  • 김정국 (고려대학교 생명과학대학)
  • Received : 2012.08.27
  • Accepted : 2012.09.28
  • Published : 2012.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study were carried out to find bolting response of cultivation in different regions and to isolate FLC (FLOWERING LOCUS C) homologs in Angelica gigas Nakai. The mean temperature of different regions, ordering in altitude, were as follows: 100 m > 350 m > 530 m > 700 m. The largest amount of rainfall was occurred in the region of 350 m while the longest time of sunshine was occurred in the region of 100 m. The content of soil chemical properties in regions showed pH 6.2 ~ 7.4, T-N 0.17 ~ 26, organic mater $1{\sim}32gkg^{-1}$, $P_2O_5$ ${151{\sim}664_{mgkg}}^{-1}$, exchangeable potassium and calcium and magnesium were 0.78 ~ 1.15, 3.9 ~ 10.0, ${0.7{\sim}3.2_{cmol}}^{+kg-1}$. L5 line of A. gigas was occurred in bolting at all regions, but the bolting ratio was 60.0% in 700 m region with non-mulching treatment. Manchu of A. gigas was not occurred in bolting at all regions. The accumulation bolting ratio of L5 line by non-mulching was higher than that of mulching as 90.4% and 72.8% in 100 m region. The MADS-box transcription factor FLC is one of the well-known examples as a strong floral repressor. We decided to isolate FLC homologs from A. gigas as a starting point of flowering mechanism research of this plant. We have isolated two RT-PCR products which showed very high amino acid sequence homology to Arabidopsis FLC.

Keywords

References

  1. Ahn SD, Yu CY and Seo JS. (1994). Effect of temperature and daylength on growth and bolting of Angelica gigas Nakai. Korean Journal of Medicinal Crop Science. 2:20-25.
  2. Boss PK, Bastow RM, Mylne JS and Dean C. (2004). Multiple pathways in the decision to flower: Enabling, promoting, and resetting. Plant Cell. 16:S18-S31. https://doi.org/10.1105/tpc.015958
  3. Chang JP, Kil GJ, Lee GH, Ji YS, Kim BR, Kang KH, Kim MR, Song MR, Park JY and Doh ES. (2011). Change of inorganic component, reducing sugar, catalpol and benzo[a]pyrene contents of Rehmannia glutinosa Libosch. var. purpurea Makino by drying methods. Korean Journal of Medicinal Crop Science. 19:501-507. https://doi.org/10.7783/KJMCS.2011.19.6.501
  4. Cho SH and Kim KJ. (1993). Inhibition of floral induction and variation of yield in Angelica gigas Nagai. Korean Journal of Crop Science. 38:151-158.
  5. Han DS. (1992). Pharmacognosy(4th ed.). Dongmyongsa Press. Seoul, Korea. p.201-202.
  6. Hepworth SR, Valverde F, Ravenscroft D, Mouradov A and Coupland G. (2002). Antagonistic regulation of flowering time gene SOC1 by CONSTANS and FLC via separate promoter motifs. EMBO Journal. 21:4327-4337. https://doi.org/10.1093/emboj/cdf432
  7. Lee SP, Cho JH, Min GG, Kwon TR, Choi JS, Park NK and Choi BS. (1995). Inhibiting floral induction of Angelica gigas Nakai in the hilly altitude area. Korean Journal of Crop Science. 40:1-8.
  8. Michaels SD and Amasino RM. (1999). FLOWERING LOCUS C encodes a novel MADS-domain protein that acts as a repressor of flowering. Plant Cell. 11:949-956. https://doi.org/10.1105/tpc.11.5.949
  9. Michaels SD and Amasino RM. (2001). Loss of FLOWERING LOCUS C activity eliminates the late-flowering phenotype of FRIGIDA and autonomous-pathway mutations, but not responsiveness to vernalization. Plant Cell. 13:935-941. https://doi.org/10.1105/tpc.13.4.935
  10. Mitsutomo A, Yasushi K, Sumiko Y, Yasufumi D, Ayako Y, Yoko I, Harutaka I, Michitaka N, Koji G, and Takashi A. (2005). FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex. Science. 309:1052-1056 https://doi.org/10.1126/science.1115983
  11. Mouradov A, Cremer F and Coupland G. (2002). Control of flowering time: Interacting pathways as a basis for diversity. Plant Cell. 14:S111-S130.
  12. Oh SK, Zhang H, Ludwig P and Nocker SV. (2004). A mechanism related to the yeast transcriptional regulator paf1c is required for expression of the Arabidopsis FLC/MAF MADS box gene family. Plant Cell. 16:2940-2953. https://doi.org/10.1105/tpc.104.026062
  13. Oh YJ, Seo HL, Choi YM and Jung DS. (2010). Evaluation of antioxidant activity of the extracts from the aerial parts of Cnidium officinale Makino. Korean Journal of Medicinal Crop Science. 18:373-378.
  14. Rouse DT, Sheldon CC, Bagnall DJ, Peacock WJ and Dennis ES. (2002). FLC, a repressor of flowering, is regulated by genes in different inductive pathways. Plant Journal. 29:183-191. https://doi.org/10.1046/j.0960-7412.2001.01210.x
  15. Samach A, Onouchi H, Gold SE, Ditta GS, Schwarz-Sommer Z, Yanofsky MF and Coupland G. (2000). Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis. Science. 288:1613-1616. https://doi.org/10.1126/science.288.5471.1613
  16. Sheldon CC, Burn JE, Perez PP, Metzger J, Edwards JA, Peacock WJ and Dennis ES. (1999). The FLF MADS box gene: A repressor of flowering in Arabidopsis regulated by vernalization and methylation. Plant Cell. 11:445-458. https://doi.org/10.1105/tpc.11.3.445
  17. Simpson GG and Dean C. (2002). Arabidopsis, the Rosetta stone of flowering time. Science. 296:285-289. https://doi.org/10.1126/science.296.5566.285
  18. Takada S and Goto K. (2003). TERMINAL FLOWER2, an Arabidopsis homolog of HETEROCHROMATIN PROTEIN1, counteracts the activation of FLOWERING LOCUS T by CONSTANS in the vascular tissues of leaves to regulate flowering time. Plant Cell. 15:2856-2865. https://doi.org/10.1105/tpc.016345
  19. Wilson RN, Heckman JW and Somerville CR. (1992). Gibberellin is required for flowering in Arabidopsis thaliana under short days. Plant Physiology. 100:403-408. https://doi.org/10.1104/pp.100.1.403
  20. Yu HS, Bang JK, Kim YG and Lee ST. (1997). Selection of Angelica gigas Nakai lines using seedling characteristics. Korean Journal of Medicinal Crop Science. 5:191-195.
  21. Yu HS, Bang JK, Kim YG, Seong NS, Lee BH and Jo JS. (2000). Effect of root head diameter of seedling on growth and bolting response in Angelica gigas Nakai. Korean Journal of Medicinal Crop Science. 8:283-289.
  22. Yu HS, Jo JS, Park CH, Park CG, Sung JS, Park HW, Seong NS and Jin DC. (2003). Plant growth and bolting affected by transplanting time in Angelica gigas. Korean Journal of Medicinal Crop Science. 11:392-396.
  23. Yu HS, Lee ST, Chang YH, Kim KS and Kim YG. (1996). Related on bolting characteristics and root yield of seeds with different bolting years in Angelica gigas Nakai. Korean Journal of Medicinal Crop Science. 4:271-276.

Cited by

  1. Effects of Eco-Friendly Organic Fertilizer on Growth and Yield of Angelica gigas Nakai vol.22, pp.2, 2014, https://doi.org/10.7783/KJMCS.2014.22.2.127