Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Comparative Analysis of Terpenoids in in vitro Culture Media of Metabolically Engineered Transgenic and Wild Type Spearmint (Mentha spicata L.)

대사 제어된 스피아민트와 야생 스피아민트 기내배양배지의 터페노이드 성분 비교 분석

  • Kang, Young-Min (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine ((KIOM)) ;
  • Park, Dong-Jin (Division of Environmental Forest Secience & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Song, Hyun-Jin (Division of Environmental Forest Secience & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Ma, Ho-Seop (Division of Environmental Forest Secience & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Karigar, Chandrakant (Department of Biochemistry, Bangalore University) ;
  • Choi, Myung-Suk (Division of Environmental Forest Secience & Institute of Agriculture and Life Science, Gyeongsang National University)
  • 강영민 (한국한의학연구원 한약기초연구그룹) ;
  • 박동진 (경상대학교 환경산림과학부) ;
  • 송현진 (경상대학교 환경산림과학부) ;
  • 마호섭 (경상대학교 환경산림과학부) ;
  • ;
  • 최명석 (경상대학교 환경산림과학부)
  • Received : 2012.07.24
  • Accepted : 2012.08.22
  • Published : 2012.10.30
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Abstract

IPP isomerase (Iso) and Limonene synthase (Limo) are important enzymes in terpenoids biosynthesis pathway. The wild type and each metabolically engineered (Iso and Limo) transgenic spearmint (Mentha spicata Linne) plants were compared for their growth patterns and the contents of essential oil in in vitro culture media. The profile of terpenoid metabolites was obtained from the essential oil of the metabolically engineered transgenic spearmint, which was extracted using a modified SDE method, by GC-MS analysis. The growth of wild spearmint was more profuse in B5 culture medium than in other media. Significant differences in leaf and root growth patterns were observed between metabolically engineered transgenic and wild type spearmint plants. The leaves of the transgenic spearmint plants were slightly elongated but were dramatically narrower than those of wild type spearmints. The content of essential oil of transgenic spearmint was different slightly depending on the target terpenoid genes. The content of essential oils in Limo transgenic plants was higher than that of Iso, except for transgenic plant in B5 medium. The transgenic spearmint produced more terpenoids than the wild type. Iso spearmint extracts showed eleven terpenoids and a phenylpropane, while Limo spearmint extracts contained nine terpenoids. However, extracts from the wild type showed the presence of only four terpenoids.

Keywords

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