KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지
DOI QR코드

DOI QR Code

Development of Extraction Process of Protein-bound Polysaccharides from Inonotus obliquus Mycelia

차가버섯 균사체로부터 단백다당체의 추출 공정 확립

  • Park, Nam-Kyu (Department of Food science & Technology, Chonbuk National University) ;
  • Chun, Gie-Taek (Division of Life Science, Kangwon National University) ;
  • Jeong, Yong-Seob (Department of Food science & Technology, Chonbuk National University)
  • Received : 2010.11.26
  • Accepted : 2012.06.20
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Inonotus obliquus mushroom, which is a fungus belonging to Hymenochaetaceae family, is known to grow on birth trees in colder northern climates and to be a fungal parasite that draws nutrients out of living trees rather than from the ground. For the separation of protein-bound polysaccharide (PBP) from the culture broth and mycelium of Inonotus obliquus, three well known extraction methods namely hot water, ultrasound and microwave were used. The best extraction conditions to separate the PBP (64.94 mg/g) from mycelium by microwave were found to be for 1 hour and $150^{\circ}C$. The possibility for concentration of extracted PBP solution by using membrane was also studied. The extracted PBP solution was concentrated effectively by using an ultrafiltration membrane and the molecular weight cut off (MWCO) is 30 KDa. It was observed that a concentration by the ultrafiltration membrane is essential not only for the development of clean separation technology but also for enhanced production of PBP. As a result, we have shown that PBP in the final concentrated solution showed approximately 10 times higher than that in the crude solution by application of the developed separation systems. The separation yield of PBP was about 89.79% by gel filtration of purification steps and the purified product was confirmed to be PBP by using FT-IR.

Keywords

References

  1. Kier, L. (1961) Triterpenes of Poria obliqua. J. Pharm Sci. 50: 471-474. https://doi.org/10.1002/jps.2600500605
  2. Shivrina, A. N. (1967) Chemical characteristics of compounds extracted from Inonotus obliquus. Chem. Abstr. 66: 17271-17279.
  3. Ludwiezak, R. S. and U. Wrzeciono (1975) Forschungen uber die cgemischen bestandteil de Inonotus obliquus, IV. Ergosterol Rocz. Chem. 34: 1701-1705.
  4. Kahlos, K. and R. Hiltunen (1983) Identification of some lanostane type triterpenes from Inonotus obliquus. Acta Pharm. Fenn. 92: 220-224.
  5. Kukulyanskaya, T. A., N. K. Kurcheno, V. P. Kurcheno, and V. G. XBabitskaya (2002) Physicochemical properties of melanins produced by the sterile from of Inonotus obliquus ("Chaga") in natural and cultivated fungus. Appl. Biochem. Microbiol. 38: 58-61. https://doi.org/10.1023/A:1013204706055
  6. Takashi, M., Z. Cun, A. Kuniaki, O. Hidehumi, K. Tadash, U. Shigeo, L. Sophie, and M. Laurent (1999) Antitumor and hypoglycemic activities of polysaccharides from the sclerotia and mycelia of Inonotus obliquus. (Pers.: Fr.) Pil. (Aphyllophoromycetidae). Int. J. Med. Mush. 1: 301-316. https://doi.org/10.1615/IntJMedMushr.v1.i4.20
  7. Shin, Y. S., Y. Tamai, and M. Terazawa (2000) Chemical constituents of Inonotus obliquus. A new triterphene; 3h-hydroxy- 8, 24-dienlanosta-21, 23-lactone from sclerotium. Eurasian. J. Fore. Res. 1: 43-50.
  8. Kahlos, K. (1996) Preliminary test of antiviral activity of two Inonotus obliquus strains. Fitoterapia LXVII 4: 344-347.
  9. Ichimura, T., T. Otake, H. Mori, and S. Maruyama (1999) HIV-1 protease inhibition and anti-HIV effect of natural and synthetic water-soluble lignin-like substance. Biosci. Biotechnol. Biochem. 63: 2202-2204. https://doi.org/10.1271/bbb.63.2202
  10. Kahlos, K. (1994) Antifungal activity of cysteine, its effect on C-21 oxygenated lanosterol derivatives and other lipid in Inonotus obliquus in vitro. Appl. Microbiol. Biotechnol. 3: 339-385.
  11. Kahlos, K., L. Kangas, and R. Hiltunen (1987) Antitumor activity of some compounds and fractions from an n-hexane extract of Inonotus obliquus. Acta Pharm. Fen. 96: 33-40.
  12. Jarosz, A., M. Skorska, J. Rzymowska, J. Kochmanska-Rdest, and E. Malarczyk (1990) Effect of the extracts from fungus Inonotus obliquus on catalase level on hela and nocardia cells. Acta Biochim. Pol. 37: 149-151.
  13. Burczyk, J., A. Gawron, M. Slotwinska, B. Smietana, and K. Terminska (1996) Antimitotic activity of aqueous extract of Inonotus obliquus. Bolle. Chim. Farm. 135: 306-309.
  14. Galasinski, W., J. Chlabicz, A. Paszkiewicz-Gadek, C. Marcinkiewicz, and A. Gindzienski (1996) The substances of plant origin that inhibit protein biosynthesis. Acta Pol. Pharm. 53: 311-318.
  15. Misuno, T. (1990) Antitumor activity and some properties of water soluble polysaccharide from fruiting body of Agaricus blasei Murill. Agric. Biol. Chem. 54: 2889-2896. https://doi.org/10.1271/bbb1961.54.2889
  16. Hwang, Y. J., G. W. Noh, and S. H. Kim (2003) Effect of Inonotus obliquus extracts on proliferation and caspase-3 activity in human gastro-intestinal cancer cell lines. J. Korean Nutr. Soc. 36: 18-30.
  17. Komatsu, N., S. Okubo, S. Kikumoto, K. Kimura, G. Saito, and S. Sasaki (1969) Host mediated antitumor action of schizophyllan, a glucan produced by Schizophyllum. Commun Gann. 60: 137-144.
  18. Tshkagoshi, S. and F. Ohashi (1974) Protein bound polysaccharide preparation, PSK, effective against mouse sarcoma-180 and ascites hepatoma AH-13 by oral use. Commun Gann. 65: 557-558.
  19. Kim, Y. O., S. B. Han, H. W. Lee, H. J. Ahn, Y. D. Yoon, J. K. Jung, H. M. Kim, and C. S. Shin (2005) Immuno-stimulating effect of the endo-polysaccharide produced by submerged culture of Inonotus obliquus. Life Sci. 77: 2438-2456. https://doi.org/10.1016/j.lfs.2005.02.023
  20. Huang, S. Q., J. W. Li, Z. Wang, H. X. Pan, J. X. Chen and Z. X. Ning (2010) Optimization of alkaline extraction of polysaccharides from Ganoderma lucidum and their effect on immune function in mice. Molecules 15: 3694-3708. https://doi.org/10.3390/molecules15053694
  21. Hong H. P. (2010) Enhanced production of polysaccharides through strain improvement and medium optimization in suspended mycelial fermentations of Inonotus obliquus, M.S. Thesis, College of Biomedical Sci., Kangwon National University, Gangwon.
  22. Pamela, M. and P. Laura (2000) Beta-glucans in edible mushrooms. Food Chem. 68: 315-318. https://doi.org/10.1016/S0308-8146(99)00197-1
  23. Vlasta, M. G., B. Romano, and J. P. Gerwin (2001) Vibrational spectroscopic characterization of wild growing mushrooms and toadstools. Spectrochimica Acta Part A 57: 2815-2829. https://doi.org/10.1016/S1386-1425(01)00584-4