International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Tetracycline-incorporated Silk Fibroin Films

  • Kim, Jong-Ho (School of Textile Engineering and Fashion Design, Kyungpook National University) ;
  • Shin, Bong-Seob (School of Textile Engineering and Fashion Design, Kyungpook National University) ;
  • Jeon, Jong-Young (School of Textile Engineering and Fashion Design, Kyungpook National University) ;
  • Kweon, Hae-Yong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Jo, You-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Heui-Sam (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Kwang-Gill (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
  • Received : 2012.08.21
  • Accepted : 2012.09.10
  • Published : 2012.09.30
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Abstract

Silk fibroin films incorporated with tetracycline was prepared and characterized by Fourier-transform infrared spectrometer and differential scanning calorimeter and examined antibacterial effect. The conformation of silk fibroin was changed from random coil to $\hat{a}$ sheet structure with incorporation of tetracycline. Antibacterial activity of the materials was evaluated against Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (Staphylococcus epidermis, and Staphylococcus aures) using agar plate method showing clear inhibition zone around tetracycline silk film. It is concluded that tetracycline-incorporated SF films are highly effective against bacteria.

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References

  1. Bardajee GR, Pourjavadi A, Soleyman R (2011) Novel nanoporous hydrogel as a carrier matrix for oral delivery of tetracycline hydrochloride. Colloids and Surface A: Physicochem Eng Aspects 392, 16-24. https://doi.org/10.1016/j.colsurfa.2011.09.014
  2. Bax CM, Shankar VS, Towhidul AA, Bax BE, Moonga BS, Huang CL, Zaidi M, Rifkin BR (1993) Tetracyclines modulate cytosolic Ca21 responses in the osteoclast associated with "Ca21 receptor" activation. Biosci Rep 13, 169-174. https://doi.org/10.1007/BF01149961
  3. Bettany JT, Wolowacz RG (1998) Tetracycline derivatives induce apoptosis selectively in cultured monocytes and macrophages but not in mesenchymal cells. Adv Dent Res 12, 136-143. https://doi.org/10.1177/08959374980120010901
  4. Guerra W, Azevedo E de A, Monteiro AR de S, Bucciarelli- Rodriguez M, Chartone-Souza E, Nascimento AMA, Fontes APS, Moyec LL, Percira-Maia EC (2005) Synthesis, characterization, and antibacterial activity of three palladium(II) complexes of tetracyclines. J Inorg Chem 99, 2348-2354.
  5. Hanawa T, Watanabe A, Tsuchiya T, Ikoma R, Hidaka M, Sugihara M (1995) New oral dosage form for elderly patients. II. Release behavior of benfotiamine from silk fibroin gel. Chem Pharm Bull 43, 872-876. https://doi.org/10.1248/cpb.43.872
  6. Krafft C, Hinrichs W, Orth P, Saenger W, Welfle H (1998) Interaction of Tet repressor with operator DNA and with tetracycline studied by infrared and raman spectroscopy. Bio-phys J 74, 63-71.
  7. Kweon H, Ha HC, Um IC, Park YH (2001) Physical Properties of Silk Fibroin/Chitosan Blend Films. J Appl Polym Sci 80, 928-934. https://doi.org/10.1002/app.1172
  8. Kweon H, Kang S, Lee K, Park K, Jo Y, Kang P, Sung G, Lee H, Shin K, Park C (2011) Solubility and conformation of silk fibroin membrane. Int J Indust Entomol 22, 101-105. https://doi.org/10.7852/ijie.2011.22.2.101
  9. Minoura N, Tsukada M, Nagura M (1990) Fine Structure and Oxygen Permeability of Silk Fibroin Membrane treated with Methanol. Polymer 31, 265-269. https://doi.org/10.1016/0032-3861(90)90117-H
  10. Minoura N, Aiba SI, Higuchi M, Gotoh Y, Tsukada M, Imai Y (1995) Attachment and growth of fibroblast cells on silk fibroin. Biochem Biophys Res Commun 208, 511-516. https://doi.org/10.1006/bbrc.1995.1368
  11. Nam J, Park YH (2001) Morphology of regenerated silk fibroin: effects of freezing temperature, alcohol addition, and molecular weight. J Appl Polym Sci 81, 3008-3021. https://doi.org/10.1002/app.1751
  12. Pruzanski W, Greenwald RA, Street IP, Laliberte F, Stefanski E, Vadas P (1992) Inhibition of enzymatic activity of phospholipases A2 by minocycline and doxycycline. Biochem Pharmacol 44, 1165-1170. https://doi.org/10.1016/0006-2952(92)90381-R
  13. Sakabe H, Ito H, Miyamoto T, Noishiki Y, Ha WS (1989) In vivo blood compatibility of regenerated silk fibroin. Sen-I Gakkaishi 45, 487. https://doi.org/10.2115/fiber.45.11_487
  14. Sasaki T, Ramamurthy NS, Golub LM (1992) Tetracycline administration increases collagen synthesis in osteoblasts of streptozotocin-induced diabetic rats: a quantitative autoradiographic study. Calcif Tissue Int 50, 411-419. https://doi.org/10.1007/BF00296771
  15. Sofia S, McCarthy MB, Gronowiza G, Kaplan DL (2001) Functional silk-based biomaterials for bone formation. J Biomed Mater Res 54, 139-148. https://doi.org/10.1002/1097-4636(200101)54:1<139::AID-JBM17>3.0.CO;2-7
  16. Trachtman H, Futterweit S, Greenwald R, Moak S, Singhal P, Franki N, Amin AR (1996) Chemically modified tetracyclines inhibit inducible nitric oxide synthase expression and nitric oxide production in cultured rat mesangial cells. Biochem Biophys Res Commun 229, 243-248. https://doi.org/10.1006/bbrc.1996.1787
  17. Yamada H, Igarashi Y, Takasu Y, Saito H, Tsubouchi K (2004) Identification of fibroin-derived peptides enhancing the proliferation of cultured human skin fibroblasts. Biomaterials 25, 467-472. https://doi.org/10.1016/S0142-9612(03)00540-4
  18. Yeo J, Lee K, Kim H, Oh Y, Kim A, Kim S (2000) The effect of PVA/chitosan/fibroin (PCF)-blended sponge on wound healing in rats. Biol Pharm Bull 23, 1220-1223. https://doi.org/10.1248/bpb.23.1220

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