International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지
DOI QR코드

DOI QR Code

Microsatellite Analysis of the Silkworm Strains (Bombyx mori) Originated from China

  • Kim, Kee-Young (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Kang, Pil-Don (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Ryu, Kang-Sun (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Kim, Ki-Hwan (Bionics) ;
  • Sung, Gyoo-Byung (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Ji, Sang-Deok (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Kim, Mi-Ja (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology) ;
  • Kim, Ik-Soo (College of Agriculture & Life Sciences, Chonnam National University)
  • Received : 2012.08.15
  • Accepted : 2012.09.04
  • Published : 2012.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

A total of 85 Chinese-origin silkworm strains preserved in Korea were genotyped for eight polymorphic micro-satellite loci. We obtained per-locus number of alleles, ranging from 5 to 14 with an average value of 9.5, perlocus observed heterozygosity, ranging from 0.07 to 0.99, and per-locus polymorphic information content (PIC), ranging from 0.34 to 0.82, indicating that some loci are highly variable. Phylogenetic analysis with the eight concatenated microsatellite loci showed no clustering on the basis of known strain characteristics. A total of 22 strain-specific apomorphic alleles, which discriminate 19 among 85 silkworm strains were obtained from eight loci. These strain-specific alleles, thus, can casually be utilized for the discrimination of applicable strains without any further typing of other loci. Furthermore, a substantial number of homozygote strains, represented by 27 among 76 alleles in eight loci were found. These results collectively suggest that the silkworm microsatellite DNA is actually and potentially important molecular markers for the eventual discrimination of silkworm strains that are preserved as hundreds in Korea.

Keywords

References

  1. Bostein D, White RL, Skolinick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphism. Am J Hum Genet 32, 314-331.
  2. Dallas JF, Dod B, Boursot P, Prager EM, Bonhomme F (1995) Population subdivision and gene flow in Danish house mice. Mole Ecol 4, 311-320. https://doi.org/10.1111/j.1365-294X.1995.tb00224.x
  3. Dharma Prasad M, Muthulakshmi M, Madhu M, Archak S, Mita K, Nagaraju J (2005) Survey and analysis of microsatellites in the silkworm, Bombyx mori: frequency, distribution, mutations, marker potential and their conservation in heterologous species. Genetics 169, 197-214.
  4. Edwards A, Civitello A, Hammond HA, Caskey CT (1991) DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 49, 746-756.
  5. Fujiwara H, Ishikawa H (1987) Structure of the Bombyx mori rDNA: initiation site for its transcription. Nucleic Acids Res 15, 1245-1258. https://doi.org/10.1093/nar/15.3.1245
  6. Huang WG, Cipriani G, Morgante M, Testolin R (1998) Microsatellite DNA in Actinidia chinensis: isolation, characterization, and homology in related species. Theor Appl Genet 97, 1269-1278. https://doi.org/10.1007/s001220051019
  7. Itoh M, Takeda S, Yamamoto H, Izumi S, Tomino S, Eguchi M (1991) Cloning and sequence analysis of membrane-bound alkaline phosphatase cDNA of the silkworm, Bombyx mori. Biochim Biophys Acta 1129, 135-138. https://doi.org/10.1016/0167-4781(91)90229-F
  8. Jin L, Chakraborty R (1994) Estimation of genetic distance and coefficient of gene diversity from single-probe multilocus DNA fingerprinting data. Mol Biol Evol 11, 120-127.
  9. Kang PD, Kim KY, Jung IY, Kim MJ, Jin BR, Kim I (2009) Character analysis of silkworm stains registered as genetic stocks in Korea. Int J Indust Entomol 19, 205-213.
  10. Kawabata T, Yasuhara Y, Ochiai M, Matsuura S, Ashida M (1995) Molecular cloning of insect pro-phenol oxidase: a copper-containing protein homologues to arthropod hemocyanin. Proc Natl Acad Sci 92, 7774-7778. https://doi.org/10.1073/pnas.92.17.7774
  11. Kim KY, Kang PD, Lee KG, Oh HK, Kim MJ, Kim K-H, Park SW, Lee SJ, Jin BR, Kim I (2010) Microsatellite analysis of the silkworm strains (Bombyx mori): high variability and potential markers for the strain identification. Genes & Genomics 32, 532-543. https://doi.org/10.1007/s13258-010-0066-x
  12. Kwok S, Kellog DE, McKinney N, Spasic D, Goda L, Levenson C, Sninsky JJ (1990) Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus 1 model studies. Nucleic Acids Res 18, 999-1005. https://doi.org/10.1093/nar/18.4.999
  13. Lee WC, Kim I (2000) The strategy for the development of bio-resources utilizing sericultural products and insects. Int J Indust Entomol 1, 95-102.
  14. Li M, Shen L, Xu A, Miao X, Hou C, Sun P, Zhang Y, Huang Y (2005) Genetic diversity among silkworm (Bombyx mori L., Lep., Bombycidae) germplasms revealed by microsatellites. Genome 48, 802-810. https://doi.org/10.1139/g05-053
  15. Lim SH, Kim HR, Lee WC, Kim SE, Mah YI, Seol GY, Kim KM, Kim JC, Chang SJ, Lee YW (1996) Principles and practices in sericulture. National Sericulture and Entomology Research Institute pp. 117-231.
  16. Liu C, Muse SV (2005) PowerMarker: integrated analysis environment for genetic marker data. Bioinformatics 21, 2128-2129. https://doi.org/10.1093/bioinformatics/bti282
  17. Mariat D, Kessler J, Vaiman LD, Panthier JJ (1996) Polymorphism characterization of five canine microsatellites. Anim Genet 27, 433-442.
  18. Michaille JJ, Mathavan S, Gaillard J, Garel A (1990) The complete sequence of mag, a new retrotransposon in Bombyx mori. Nucleic Acids Res 18, 674. https://doi.org/10.1093/nar/18.3.674
  19. Morgante M, Olivieri AM (1993) PCR-amplified microsatellites as markers in plant genetics. Plant J 3, 175-182. https://doi.org/10.1111/j.1365-313X.1993.tb00020.x
  20. Nei M (1987) Estimation of average heterozygosty and genetic distance from a small number of individuals. Genetics 89, 583-590.
  21. Ohta T, Kobayashi M, Hirose S (1995) Cloning of a cDNA for DNA supercoiling factor reveals a distinctive Ca(2+)-binding protein. J Biol Chem 270, 15571-15575. https://doi.org/10.1074/jbc.270.26.15571
  22. Pope LC, Sharp A, Moritz C (1996) Population structure of the yellow-footed rock-wallby Petrogale xanthopus (Gray, 1854) inferred from mtDNA sequences and microsatellite loci. Mole Ecol 5, 629-640. https://doi.org/10.1111/j.1365-294X.1996.tb00358.x
  23. Reddy KD, Abraham EG, Nagaraju J (1999) Microsatellites in the silkworm, Bombyx mori: abundance, polymorphism, and strain characterization. Genome 42, 1057-1065. https://doi.org/10.1139/gen-42-6-1057
  24. Romero C, Pedryc A, Muñoz V, Llácer G, Badenes ML (2003) Genetic diversity of different apricot geographical groups determined by SSR markers. Genome 46, 244-252. https://doi.org/10.1139/g02-128
  25. Rozen S, Skaletsky H (2000) PRIMER3 on the WWW for general users and for biologist programmer. in Bioinformatics Methods and Protocols in the series Methods in Molecular Biology. Krawetz S, Misener S (eds.) pp. 365-386, Humana Press, Totowa, NJ.
  26. Ryu KS, Kim I, Ahn MY, Lee HS, Kim JW, Lee P (2003) Functionality research on silkworm and sericultural products. Food Sci Indust 36, 15-24.
  27. Tautz D, Renz M (1984) Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Res 12, 4127-4138. https://doi.org/10.1093/nar/12.10.4127
  28. Viard F, Bremond P, Labbo R, Justy F, Delay B, Jarne P (1996) Microsatellites and the genetics of highly selfing populations in the fresh water snail Bulinus trucatus. Genetics 142, 1237-1247.
  29. Weber JL, May PE (1989) Abundant class of human DNA polymorphisms. Genomics 44, 388-396.
  30. Weir BS (1996) Genetic Data Analysis II. Sinauer Associates, Inc, Sunderland, MA.
  31. Woodruff DE (1993) Non-invasive genotyping of primates. Primates 34, 333-346. https://doi.org/10.1007/BF02382629
  32. Yue GH, Orban L (2000) Rapid isolation and characterization of microsatellites from the genome of Asian arowana(Scleropages formosus, Osteoglossidae, Pisces). Mole Ecol 9, 1007-1009. https://doi.org/10.1046/j.1365-294x.2000.00939-10.x

Cited by

  1. Microsatellite Analysis of Silkworm Strains (Bombyx mori) of Japan Origin Preserved in Korea vol.28, pp.2, 2014, https://doi.org/10.7852/ijie.2014.28.2.39
  2. Role of microsatellites in genetic analysis of Bombyx mori silkworm: a review vol.8, pp.None, 2012, https://doi.org/10.12688/f1000research.20052.1