한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제35권3호
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  • Pages.169-174
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  • 2012
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지
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Evaluation of Japanese encephalitis virus vaccine strains currently used in pigs by molecular characterization

  • Lee, Jeong-Ah (Animal, Plant and Fishery Quarantine Inspection and Agency, MIFAFF) ;
  • Yang, Dong-Kun (Animal, Plant and Fishery Quarantine Inspection and Agency, MIFAFF) ;
  • Kim, Ha-Hyun (Animal, Plant and Fishery Quarantine Inspection and Agency, MIFAFF) ;
  • Kim, Sun-Young (Animal, Plant and Fishery Quarantine Inspection and Agency, MIFAFF) ;
  • Nah, Jin-Ju (Animal, Plant and Fishery Quarantine Inspection and Agency, MIFAFF) ;
  • Cho, Soo-Dong (Animal, Plant and Fishery Quarantine Inspection and Agency, MIFAFF) ;
  • Song, Jae-Young (Animal, Plant and Fishery Quarantine Inspection and Agency, MIFAFF)
  • 투고 : 2012.08.07
  • 심사 : 2012.08.28
  • 발행 : 2012.09.30
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초록

Japanese encephalitis virus (JEV) is one of the main causes of viral encephalitis in human and animals. For over 30 years, a live attenuated JEV vaccine strain has been used in the veterinary field, and it is required to conduct quality evaluation studies on the commercial vaccines. For the quality control of live attenuated JEV vaccine, we investigated the nucleotide sequence similarity of prME gene derived from five JEV vaccines commercially available in pigs in Korea. The Vero cells infected with JEV vaccines showed specific cytopathic effect, which was characterized by rounding and detached cells. In the phylogenetic analysis, all of the vaccine strains showed a close relationship with the original vaccine seed strain (Anyang 300) and clustered into the genotype 3. In comparison of the nucleotide and deduced amino acid sequences of prME genes with the original strain, all JEV vaccine strains showed high amino acid similarity ranging from 98.9% to 99.5%, but had several point mutations, probably due to high mutation rates of viral RNA polymerase by several virus passages. Even though the current JEV vaccine strains have been maintained and produced for a long period of time, the genetic characterization of them have been rarely changed. However, since the mid 1990's, molecular epidemiology of JEV has been changed sharply from genotype 3 to genotype 1 in Korea, further studies on new vaccine strains to genotype 1 is required for more effective prevention in the field.

키워드

참고문헌

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피인용 문헌

  1. The present and future of veterinary vaccines for Japanese encephalitis in Korea vol.4, pp.2, 2015, https://doi.org/10.7774/cevr.2015.4.2.130