Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Isolation of Anaerobic Cellulolytic Bacteria from the Rumen of Holstein Dairy Cows to Develop Feed Additives for Ruminants

반추동물용 사료첨가제개발을 위한 홀스타인 젖소의 반추위로부터 분리한 혐기성 섬유소 분해균의 특성연구

  • Choi, Nag-Jin (The Institute of Rare Earth for Biological Application, Department of Animal Science, Chonbuk National University) ;
  • Lee, Gi-Young (Graduate School of Biotechnology, Environmental and Information Technology, Hankyong National University) ;
  • Jeong, Kwang-Hwa (National Institute of Animal Science, RDA) ;
  • Kim, Chang-Hyun (School of Animal Life and Environment Science, Biogas Research Center, Hankyong National University)
  • Received : 2012.08.21
  • Accepted : 2012.09.15
  • Published : 2012.09.28
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Abstract

In order to develop a high cellulolytic direct-fed microorganism (DFM) for ruminant productivity improvement, this study isolated cellulolytic bacteria from the rumen of Holstein dairy cows, and compared their cellulolytic abilities via DM degradability, gas production and cellulolytic enzyme activities. Twenty six bacteria were isolated from colonies grown in Dehority's artificial (DA) medium with 2% agar and cultured in DA medium containing filter paper at $39^{\circ}C$ for 24h. 16s rDNA gene sequencing of four strains from isolated bacteria showed that H8, H20 and H25 strains identified as Ruminococcus flavefaciens, and H23 strain identified as Fibrobacter succinogenes. H20 strain had higher degradability of filter paper compared with others during the incubation. H8 (R. flavefaciens), H20 (R. flavefaciens), H23 (F. succinogenes), H25 (R. flavefaciens) and RF (R. flavefaciens sijpesteijn, ATCC 19208) were cultured in DA medium with filter paper as a single carbon source for 0, 1, 2, 3, 4 and 6 days without shaking at $39^{\circ}C$, respectively. Dry matter degradability rates of H20, H23 and H25 were relatively higher than those of H8 and RF since 2 d incubation. The cumulative gas production of isolated cellulolytic bacteria increased with incubation time. At every incubation time, the gas production was highest in H20 strain. The activities of carboxymethylcellulase (CMCase) and Avicelase in the culture supernatant were significantly higher in H20 strain compared with others at every incubation time (p<0.05). Therefore, although further researches are required, the present results suggest that H20 strain could be a candidate of DFM in animal feed due to high cellulolytic ability.

Keywords

References

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