International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Identification of differentially expressed genes using an annealing control primer system in periodontitis

  • Na, Hee-Sam (Department of Oral Microbiology, School of Dentistry, Pusan National University) ;
  • Kim, Ji-S. (Department of Oral Microbiology, School of Dentistry, Pusan National University) ;
  • Chung, Jin (Department of Oral Microbiology, School of Dentistry, Pusan National University)
  • Received : 2012.08.22
  • Accepted : 2012.09.12
  • Published : 2012.09.30
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Abstract

In the gingival tissues of patients with periodontitis, inflammatory responses are mediated by a wide variety of genes. In this study, we screened for differentially expressed genes (DEGs) in periodontitis compared with normal tissue using an annealing control primer (ACP) system. By ACP RT-PCR analysis, we obtained about 160 amplicons, 8 of which were found to be differentially expressed. DEGs in patients with periodontitis were thus successfully and reliably identified by the ACP-based RT PCR technique. The DEGs identified in the screen may also enhance our understanding of the pathogenesis of periodontitis.

Keywords

References

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