Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Effects of White Radish (Raphanus sativus) Enzyme Extract on Hepatotoxicity

  • Lee, Sang-Wha (Department of Microbiology, College of Medicine, Dong-A University) ;
  • Yang, Kwang-Mo (Department of Research Center, Dong Nam Institute of Radiological & Medical Sciences) ;
  • Kim, Jung-Ki (Department of Research Center, Dong Nam Institute of Radiological & Medical Sciences) ;
  • Nam, Byung-Hyouk (Test and Certification Team, Marine Bio-industry Development Center) ;
  • Lee, Chang-Min (Department of Microbiology, College of Medicine, Dong-A University) ;
  • Jeong, Min-Ho (Department of Microbiology, College of Medicine, Dong-A University) ;
  • Seo, Su-Yeong (Department of Microbiology, College of Medicine, Dong-A University) ;
  • Kim, Gi-Yong (Department of Microbiology, College of Medicine, Dong-A University) ;
  • Jo, Wol-Soon (Department of Research Center, Dong Nam Institute of Radiological & Medical Sciences)
  • Received : 2012.08.08
  • Accepted : 2012.09.25
  • Published : 2012.09.30
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Abstract

Raphanus sativus (Cruciferaceae), commonly known as radish is widely available throughout the world. From antiquity it has been used in folk medicine as a natural drug against many toxicants. The present study was designed to evaluate the hepatoprotective activity of radish (Raphanus sativus) enzyme extract (REE) in vitro and in vivo test. The $IC_{50}$ values of REE in human liver derived HepG2 cells was over 5,000 ${\mu}g/ml$ in tested maximum concentration. The effect of REE to protect tacrine-induced cytotoxicity in HepG2 cells was evaluated by MTT assay. REE showed their hepatoprotective activities on tacrine-induced cytotoxicity and the $EC_{50}$ value was 1,250 ${\mu}g/ml$. Silymarin, an antihepatotoxic agent used as a positive control exhibited 59.7% hepatoprotective activity at 100 ${\mu}g/ml$. Moreover, we tested the effect of REE on carbon tetrachloride ($CCl_4$)-induced liver toxicity in rats. REE at dose of 50 and 100 mg/kg and silymarin at dose of 50 mg/kg were orally administered to $CCl_4$-treated rats. The results showed that REE and silymarin significantly reduced the elevated levels of serum enzyme markers induced by $CCl_4$. The biochemical data were supported by evaluation with liver histopathology. These findings suggest that REE, can significantly diminish hepatic damage by toxic agent such as tacrine or $CCl_4$.

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