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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells

  • Kim, Dong-Bum (Department of Microbiology, College of Medicine, Hallym University) ;
  • Park, Min-Chul (Department of Microbiology, College of Medicine, Hallym University) ;
  • Park, Byoung-Kwon (Department of Microbiology, College of Medicine, Hallym University) ;
  • Kwon, Sang-Hoon (Center for Medical Science Research, College of Medicine, Hallym University) ;
  • Choi, Joon-Ho (Department of Food Science and Biotechnology, College of Life Science and Natural Resources, Wonkwang University) ;
  • Kim, Hyun-Jong (Experimental Animal Center, Hallym University) ;
  • Choi, Soo-Young (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Park, Jin-Seu (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Lee, Young-Hee (Department of Biochemistry, College of Natural Science, Chungbuk National University) ;
  • Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University)
  • Received : 2012.03.06
  • Accepted : 2012.03.23
  • Published : 2012.09.30
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Abstract

We evaluated the expression of the costimulatory molecules CD80 and CD83 and major histocompatibility (MHC) class II induced by 2,4-dinitrofluorobenzene (DNFB) in the RAW 264.7 macrophage cell line. In contrast to the previously reported effect of DNFB on dendritic cells, CD86 expression did not change. Furthermore, we observed that the CD83 expression level transiently increased and then decreased. Induction of CD80 and MHC class II molecule expression and a decrease in CD83 expression by DNFB in vitro were also confirmed in splenocytes of BALB/c and NC/Nga mice. However, DNFB did not influence CD83 expression in peritoneal $CD11b^+$ cells from BALB/c or NC/Nga mice. Detailed in vivo experiments and further studies on the possible contribution of $CD11b^+$ cells to induce atopic dermatitis (AD) would be helpful to attain a better understanding of AD pathogenesis.

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References

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