The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of CJB Water Extract on Obesity-Related Factors in Hypothalamus of Rats Fed High-Fat Diet

고지방식이 유도 비만 흰쥐의 뇌 시상하부 비만 관련 인자에 대한 차전자와 복령의 복합 물추출물의 효과

  • Hwang, Jeong-Soo (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Suk, Jang-Mi (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Choi, Hye-Min (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Shin, In-Soon (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Hwang, Su-Jung (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Park, Ji-Young (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Kim, Sung-Ok (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Seo, Bu-Il (Department of Herbal Pharmacology, Daegu Haany University) ;
  • Kim, Mi-Ryeo (Department of Herbal Pharmacology, Daegu Haany University)
  • 황정수 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 석장미 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 최혜민 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신인순 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 황수정 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 박지영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김성옥 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 서부일 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2012.08.09
  • Accepted : 2012.09.05
  • Published : 2012.09.30
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Abstract

Objectives : This study was conducted to investigate the anti-obesity effects of mixed water extract of Plantaginis Semen & Poria (CJB) on obese rats induced with high fat diet. Method: Male Sprague-Dawley rats were divided into three groups; Normal group, high-fat (HF) group, HF+CJB(100 mg/kg, P.O.) for 8 weeks. The body weight, food intake and weights of adipose tissues were measured, respectively. Lipid profiles in serum were analyzed by automatic analyzer of blood. Obese marker proteins and the changes of NPY and LR immunoreactivities in hypothalamus were analyzed by Western blot and immunohistochemistry. Results : CJB significantly reduced body weight, food intake, adipose tissue weights compared to HF group. Serum triglyceride and total cholesterol were significantly higher in HF group than in Normal group however, CJB significantly lowered those of HF group. HDL-cholesterol level in CJB groups was elevated compared to HF group. The pAMPK in hypothalamus were decreased in that of HF group and that of CJB group decreased. Inversely, ACC was increased in HF group and that of CJB groups decrease. Expression of $PPAR{\gamma}$ in hypothalamus was increased by CJB treatment. However, $PPAR{\alpha}$ levels in CJB group were decreased compared to HF group. The expressions of NPY and LR in PVN and ARC of hypothalamus were decreased in CJB group, respectively. Conclusion : Administration of CJB can play anti-obesity through regulations of NPY and LR activities and obesity marker proteins in obese rat's hypothalamus.

Keywords

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