생명과학회지 (Journal of Life Science)

  • 제22권9호
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  • Pages.1207-1213
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  • 2012
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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건강기능성식품 기능성원료로서 양파껍질추출물의 품질특성

Potential of Onion Peel Extract as a Functional Ingredient for Functional Foods

  • 전선영 (창원대학교 식품영양학과) ;
  • 백정화 (창원대학교 식품영양학과) ;
  • 정은정 (창원대학교 식품영양학과) ;
  • 차용준 (창원대학교 식품영양학과)
  • Jeon, Seon-Young (Department of Food and Nutrition, Changwon National University) ;
  • Baek, Jeong-Hwa (Department of Food and Nutrition, Changwon National University) ;
  • Jeong, Eun-Jeong (Department of Food and Nutrition, Changwon National University) ;
  • Cha, Yong-Jun (Department of Food and Nutrition, Changwon National University)
  • 투고 : 2012.06.16
  • 심사 : 2012.08.24
  • 발행 : 2012.09.30
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초록

본 연구에서는 양파가공부산물인 양파껍질을 이용하여 기능성 식품소재로 개발하고자 양파껍질추출물을 제조하였으며 양파껍질추출물에 대한 생리적 기능성과 같은 품질 특성을 조사하였다. 양파껍질추출물에서는 K가 13,767.56~15,506.78 ppm으로 가장 많은 함량을 차지하였고 Na이 8,602.44~9,796.00 ppm, Ca은 4,255.78~4,903.33 ppm으로 세 번째로 많은 함량을 차지하였다. 그리고 Mg, P, Fe은 각각 1,433.11~1,561.22 ppm, 1,212.44~1,428.89 ppm, 760.67~858.89 ppm의 함량을 차지하였고 Zn은 34.11~66.89 ppm으로 미량 검출되었다. Total phenol 함량은 598.57~626.73 mg/g, total flavonoid 함량은 211.73~238.52 mg/g 범위로 quercetin 함량은 93.67~107.29 mg/g의 범위로 나타났다. 양파껍질추출물의 DPPH radical 소거활성은 100 ppm에서 16.45~17.92%, 200 ppm은 29.96~35.41%, 1,000 ppm은 81.05~84.60%의 소거활성을 보였고, SOD 유사활성은 10,000 ppm에서 31.92~39.29%, 20,000 ppm에서 85.85~91.58%의 활성을 나타내었다. 혈전용해능은 20,000 ppm 농도에서는 plasmin에 비해 약 3배 높은 활성을 가졌다. 따라서 양파껍질 추출물은 항산화력 및 항혈전효과능을 가진 기능성소재로서의 활용 가능성을 보였다.

Onion peels is a natural source of high-value functional ingredients produced in the onion industry without suitable processing. The objective of this study was to evaluate characteristics of onion peel extract (OPE), including its biological activities, obtained from solvent extraction in 3 times pilot scales (Lot A, B, and C). Mineral analysis showed that K was present in the largest amount (13,767.56-15,506.78 ppm), followed by Na and Ca at 8,602.44-9,796.00 ppm and 4,255.78-4,903.33 ppm, respectively. The amounts of total phenol, total flavonoid, and quercetin in the OPE were in the ranges of 598.57~626.73, 211.73~233.64, and 93.78~107.29 mg/g, respectively. The biological activities such as antioxidant and effects of fibrinolysis increased in parallel with the concentration of OPE. The $IC_{50}$ value of DPPH radical scavenging activity was in the range of 517.58~557.32 ppm in the OPE. The $IC_{50}$ value for superoxide dismutase (SOD)-like activity was in the range of 11,900.91~12,690.35 ppm. A clear zone of OPE (20,000 ppm) in fibrinolysis test was three times higher than the plasmin as a reference. In conclusion, OPE could be used as a good source of antioxidants and fibrinolytic activities.

키워드

참고문헌

  1. AOAC. 1995. Official methods of analysis. 16th ed. pp, 69-74, Association of official analytical chemists, Washington DC.
  2. Ariyama, K., Nishida, T., Noda, T., Kadokura, M. and Yasui, A. 2006. Effects of fertilization, crop year, variety and provenance factors on mineral concentration in onions. J. Agric. Food Chem. 54, 3341-3350. https://doi.org/10.1021/jf0525481
  3. Astrup, T. and Mullertz, S.1952. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40, 346-351. https://doi.org/10.1016/0003-9861(52)90121-5
  4. Augusti, K. T. 1996. Therapeutic values onions (Allium cepa L.) and garlic (Allium sativum L). Indian J. Exp. Biol. 34, 634-640.
  5. Bang, H. A. and Cho, J. S. 1998. Antioxidant effects on various solvent extracts from onion peel and onion flesh. J. Korea Nutr. Soc. 4, 14-19.
  6. Basra, A. S., Singh, B. and Malik, C. P. 1994. Amelioration of the effects of ageing in onion seeds by osmotic priming and associated changes in oxidative metabolism. Biologia. Plantarum 36, 365-371. https://doi.org/10.1007/BF02920933
  7. Bilyk, A. D., Cooper, P. L. and Sapers, G. M. 1984. Varietal differences in distribution of quercetin and kaempferol in onion (Allium cepa L.) tissue. J. Agric. Food Chem. 32, 274-276. https://doi.org/10.1021/jf00122a024
  8. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181, 1199-1200. https://doi.org/10.1038/1811199a0
  9. Cha, Y. J., Jeong, E. J., Jeon, S. Y. and Baek, J. H. 2011. Method for preparation of onion process residuum extract having antioxidant and fibrinolysis. Korean Patent. 10-1101189.
  10. Chen, J. H. and Ho, C. T. 1997. Antioxidant activities of caffeic acid and its related hydroxycinnamic acid compounds. J. Agric. Food Chem. 45, 2374-2378. https://doi.org/10.1021/jf970055t
  11. Dewanto, V., Wu, X., Adom, K. K. and Liu, R. H. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agric. Food Chem. 50, 3010-3014. https://doi.org/10.1021/jf0115589
  12. Herrmann, K. 1976. Flavonols and flavones in food plants, A review. J. Food Tech. 11, 433-448. https://doi.org/10.1111/j.1365-2621.1976.tb00743.x
  13. Hubbard, G. P., Stevens, J. M., Cicmil, M., Sage, T., Jordan, P. A., Williams, C. M., Lovegrove, J. A. and Gibbins, J. M. 2003. Quercetin inhibits collagen-stimulated platelet activation through inhibition of multiple components of the glycoprotein VI signaling pathway. J. Thromb. Haemost. 1, 1079-1088. https://doi.org/10.1046/j.1538-7836.2003.00212.x
  14. Hubbard, G. P., Wolffram, S., de Vos, R., Bovy, A., Gibbins, J. M. and Lovegrove, J. A. 2006. Ingestion of onion soup high in quercetin inhibits platelet aggregation and essential components of the collagen-stimulated platelet activation pathway in man: a pilot study. Br. J. Nutr. 96, 482-488.
  15. Hubbard, G. P., Wolffram, S., Lovegrove, J. A. and Gibbins, J. M. 2003. The role of polyphenolic compounds in the diet as inhibitors of platelet function. Proc. Nutr. Soc. 62, 468-478.
  16. Iqbal, S., Bhanger, M. I. and Anwar, F. 2005. Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chem. 93, 265-272. https://doi.org/10.1016/j.foodchem.2004.09.024
  17. Jang, E. K., Seo, J. H. and Lee, S. P. 2008. Physiological activity and antioxidative effects of aged black garlic (Allium sativum L.) Extracts. Korean J. Food Sci. Technol. 40, 443-448.
  18. Jang, H. S. 2009. Quality stability of concentrated onion extracts having biological activity during storage. MS Thesis. Changwon National University. Changwon, Korea.
  19. Jeon, S. Y., Baek, J. H., Jeong, E. J. and Cha, Y. J. 2012. Optimal extraction conditions of flavonoids from onion peels via response surface methodology. J. Korean Soc. Food Nutr. 41, 695-699. https://doi.org/10.3746/jkfn.2012.41.5.695
  20. Jeon, S. Y., Jeong, E. J., Baek, J. H. and Cha, Y. J. 2011. Analytical method validation of quercetin in changnyeong onion extract as a functional ingredient for functional health food. J. Korean Soc. Food Sci. Nutr. 40, 565-569. https://doi.org/10.3746/jkfn.2011.40.4.565
  21. Jeong, C. H., Kim, J. H. and Shim, K. H. 2006. Chemical components of yellow and red onion. J. Korean Soc. Food Sci. Nutr. 35, 708-712. https://doi.org/10.3746/jkfn.2006.35.6.708
  22. Jeong, I. Y. 2005. Antioxidant activity and radioprotection of two flavonoids from propolis. J. Korean Soc. Food Sci. Nutr. 34, 162-166. https://doi.org/10.3746/jkfn.2005.34.2.162
  23. KFDA. 2008. Health functional food code. Korea Food and Drug Administration, pp. III.3.6.3-1-2, Seoul, Korea.
  24. KFDA. 2009. Korea Food Code. Korea Food and Drug Administration, pp. 386-387, Seoul, Korea.
  25. KFDA. 2009. Korea Food Code. Korea Food and Drug Administration, pp. 676-683, Seoul, Korea.
  26. KFDA. 2011. Bulletin of Korea Food and Drug Administration No. 2011-34. pp. 9-11.
  27. Kim, S. J. and Kim, G. H. 2006. Quantification of quercetin in different parts of onion and its DPPH radical scavenging and antibacterial activity. Food Sci. Biotechnol. 15, 39-43.
  28. Kim, S. K. and Kim, M. K. 2004. Effect of dried powders or ethanol extracts of onion flesh and peel on lipid metabolism, antioxidative and antithrombogenic capacities in 16-month-old rats. Korean J. Nutr. 37, 623-632.
  29. Leighton, T., Ginther, C., Fluss, L., Harter, W. K., Cansado, J. and Notario, V. 1992. Molecular characterization of quercetin and quercetin glycosides in Allium vegetables. Phenolic Compounds in Food and Their Effects on Health II, pp. 220. ACS, Washington, D.C.
  30. Marklund, S. and Marklund, G. 1974. Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  31. Park, P. S. and Lee, M. Y. 1992. The Effects of Onion and Garlic on Copper- Phenanthroline Complex Induced DNA Degradation. J. Korean Soc. Food Nutr. 21, 367-371.
  32. Park, Y. K. 1995. Source and processing technology of vegetable juice and the trend of study. Bull. Food Techonol. 8, 59-68.
  33. Rice-Evans, C. A., Miller, N. J., Bolwell, P. G., Bremley, P. M. and Pridham, J. B. 1995. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic. Res. 22, 375-383. https://doi.org/10.3109/10715769509145649
  34. Singleton, V. L., Orthofer, R. and Lamuela-Raventos. R. M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin -ciocalteu reagent. Methods Enzymol. 299, 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
  35. Shin, J. H., Lee, J. Y., Ju, J. C., Lee, S. J., Cho, H. S. and Sung, N. J. 2005. Chemical properties and nitrite scavenging ability of Citron (Citrus junos). J. Korean Soc. Food Sci. Nutr. 34, 496-502. https://doi.org/10.3746/jkfn.2005.34.4.496
  36. Vanderhoek, J. Y., Makheja, A. N. and Bailey, J. M. 1980. Inhibition of fatty acid oxygenases by onion and garlic oils. Evidence for the mechanism by which these oils inhibit platelet aggregation. Biochem. Pharmacol. 29, 3169-3173. https://doi.org/10.1016/0006-2952(80)90581-X

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