Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Increased in vitro Anticancer Effects of Potassium Bamboo Salt

칼륨죽염의 in vitro 항암 기능성 증진 효과

  • Zhao, Xin (Dept. of Food Science and Nutrition, Pusan National University) ;
  • Jeong, Ji-Kang (Dept. of Food Science and Nutrition, Pusan National University) ;
  • Kim, So-Young (Functional Food & Nutrition Division, Dept. of Agrofood Resources, National Academy of Agricultural Sciences (NAAS), RDA) ;
  • Park, Kun-Young (Dept. of Food Science and Nutrition, Pusan National University)
  • 조흔 (부산대학교 식품영양학과) ;
  • 정지강 (부산대학교 식품영양학과) ;
  • 김소영 (농촌진흥청 국립농업과학원 농식품자원부 기능성식품과) ;
  • 박건영 (부산대학교 식품영양학과)
  • Received : 2012.06.05
  • Accepted : 2012.07.09
  • Published : 2012.09.30
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Abstract

Potassium added with bamboo salt showed better antioxidative effects than bamboo salt, solar salt, or purified salt. It also showed inhibitory effects on the mutagenicity of MNNG (N-methyl-N-nitro-N-nitrosoguanidine) in a Salmonella Typhimurium TA100 tester strain. At concentrations of 1.25 and 2.5 mg/plate, potassium bamboo salt and bamboo salt showed weaker co-mutagenicity effects than either purified salt or solar salt, respectively. Anticancer effects of salts were evaluated using MTT assay in HCT-116 human colon carcinoma cells. At a 1% salt concentration, the growth inhibitory rate of potassium bamboo salt was 54%, higher than that of 1 time baked bamboo salt (36%). However, purified salt and solar salt showed relatively lower inhibitory effects of 19% and 23%, respectively. To determine the inhibitory mechanisms of potassium bamboo salt, the expression levels of Bax and Bcl-2 genes in HCT-116 cells were determined by RT-PCR. Potassium bamboo salt significantly increased Bax and decreased Bcl-2 expression levels unlike bamboo salt, purified salt, and solar salt (p<0.05). Therefore, addition of potassium to salt decreased co-mutagenicity and increased in vitro antioxidative and anticancer effects.

칼륨을 첨가하여 제조한 죽염의 항암효과를 일반죽염 및 천일염, 정제염과 비교하여 칼륨 첨가의 영향을 알아보고자 하였다. DPPH radical 소거능을 측정한 결과, 칼륨죽염과 죽염의 radical 소거능이 높았고, 칼륨죽염의 소거능이 가장 높게 나타났다. 정제염과 천일염의 소거능은 상대적으로 낮았고 칼륨죽염과 20배 이상의 차이가 있었다. MNNG를 사용한 Salmonella Typhimurium TA100 돌연변이 실험결과 소금은 모두 보돌연변이 효과가 있었지만, 칼륨죽염의 보돌연변이 효과가 다른 소금보다 3~46% 낮게 나타났다. HCT-116 암세포에 성장 억제 효과에 있어서도 위와 비슷한 경향을 나타내었는데, 칼륨죽염과 일반죽염의 암세포 성장억제효과와 암세포의 apoptosis 유도 효과가 천일염 및 정제염에 비해 높았고 그중 칼륨죽염의 in vitro 항암 기능성이 가장 우수하였다. 이상의 결과로부터 죽염의 항암효과는 천일염이 정제염보다 좋으며, 이러한 죽염의 항암효과를 칼륨을 첨가함으로써 더욱 증진될 수 있는 것으로 나타났다. 본 연구에서 죽염의 항암 기능성이 증가한 주요 원인은 칼륨이라고 생각되어지며, 이것이 죽염으로 제조될 때 그 효과가 더 증진되는 것으로 보인다.

Keywords

References

  1. Livingston VJ. 2005. Agriculture and soil pollution: new research. Nova Publishers, New York, NY, USA. p 45.
  2. Cho SD, Kim GH. 2010. Changes of quality characteristics of salt-fermented shrimp prepared with various salts. Korean J Food & Nutr 23: 291-298.
  3. Kim YH, Ryu HI. 2003. Elements in a bamboo salt and comparison of its elemental contents with those in other salts. Yakhak Hoeji 47: 135-141.
  4. Ha JO, Park KY. 1998. Comparison of mineral content and external structure of various salts. J Korean Soc Food Sci Nutr 27: 413-418.
  5. Ahn KY, Kim BY, Lee SW. 1999. Renal adaptive responses of $HK_{{\alpha}2}$ gene ($HK_{{\alpha}2a}$, $HK_{{\alpha}2b}$) to the changes of potassium (K) diet. Korean J Nephrol 18: 672-682.
  6. Shin KN, Lee HS, Kwon CS. 2011. Effects of nutrition education in type 2 diabetes mellitus on diabetes control and blood antioxidant status. J Korean Soc Food Sci Nutr 40: 689-695. https://doi.org/10.3746/jkfn.2011.40.5.689
  7. Newmark P. 1985. Events at the surface of the cell. Nature 317: 380. https://doi.org/10.1038/317380a0
  8. Cameron IL, Smith NK, Pool TB, Sparks RL. 1980. Intracellular concentration of sodium and other elements as related to mitogenesis and oncogenesis in vivo. Cancer Res 40: 1493-1500.
  9. Tutton PJ, Barkla DH. 1980. The influence of dibutyryl adenosine cyclic monophosphate on cell proliferation in the epithelium of the jejunal crypts, the colonic crypts and colonic carcinoma of rat. Clin Exp Pharmacol Physiol 7: 275-280. https://doi.org/10.1111/j.1440-1681.1980.tb00071.x
  10. Newmark HL, Wargovich MJ, Bruce WR, Boynton AL, Klein LP, Whitfield JF, Jansson B, Camerson IL. 1985. Ions and neoplastic development. In Large Bowel Cancer. Mastromarino AJ, Brattain MG, eds. Praeger, New York, NY, USA. p 102-131.
  11. Nagy I, Lustyik G, Lukacs G, Nagy V, Balazs G. 1983. Correlation of malignancy with the intracellular $Na^+$:$K^+$ ratio in human thyroid tumors. Cancer Res 43: 5395-5402.
  12. Han GJ, Choi HS, Lee SM, Lee EJ, Park SE, Park KY. 2011. Addition of starters in pasteurized brined baechu cabbage increased kimchi quality and health functionality. J Korean Soc Food Sci Nutr 40: 110-115. https://doi.org/10.3746/jkfn.2011.40.1.110
  13. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat Res 113: 173-215. https://doi.org/10.1016/0165-1161(83)90010-9
  14. Park JG, Kramer BS, Steinberg SM, Carmichael J, Collins JM, Minna JD, Gazdar AF. 1987. Chemosensitivity testing of human colorectal carcinoma cell lines using a tetrazolium-based colorimetric assay. Cancer Res 47: 5875-5879.
  15. Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR. 1990. New colorimetric cytotoxicity assay for anticancerdrug screening. J Natl Cancer Inst 82: 1107-1112. https://doi.org/10.1093/jnci/82.13.1107
  16. Bak SS, Kong CS, Rhee SH, Rho CW, Kim NK, Choi KL, Park KY. 2007. Effect of sulfur enriched young radish kimchi on the induction of apoptosis in AGS human gastric adenocarcinoma cells. J Food Sci Nutr 12: 79-83.
  17. Zhao X, Song JL, Lee JH, Kim SY, Park KY. 2010. Antioxidation and cancer cell (HT-29) antiproliferation effects of Rubus coreanus Miquel bamboo salt. Cancer Prev Res 15: 306-312.
  18. Kim SH, Park KY, Suh MJ. 1995. Comutagenic effect of sodium chloride in Salmonella/mammalian microsome assay. Food Biotechnol 4: 264-267.
  19. Zhao X, Kim SH, Qi Y, Kim SY, Park KY. 2012. Effects of different kinds of salt in the comutagenicity and growth of cancer cells. J Korean Soc Food Sci Nutr 41: 26-32. https://doi.org/10.3746/jkfn.2012.41.1.026
  20. Jung KO, Lee SM, Rhee SH, Park KY. 2001. Reduced comutagenic and antimutagenic effects of bamboo salt prepared with KCl and NaCl. J Korean Cancer Prev 6: 140-147.
  21. Jansson B. 1981. Seneca country, New York: an area with low cancer mortality rates. Cancer 48: 2542-2546. https://doi.org/10.1002/1097-0142(19811201)48:11<2542::AID-CNCR2820481133>3.0.CO;2-#
  22. Kune GA, Kune S, Watson LF. 1989. Dietary sodium and potassium in take and colorectal cancer risk. Nutr Cancer 12: 351-359. https://doi.org/10.1080/01635588909514036
  23. Tuyns AJ. 1988. Salt and gastrointestinal cancer. Nutr Cancer 11: 229-232. https://doi.org/10.1080/01635588809513992
  24. Graham S, Dayal H, Swanson M, Mittelman A, Wilkinson G. 1978. Diet in the epidemiology of cancer of the colon and rectum. J Natl Cancer Inst 61: 709-714.
  25. Park KY, Cho EJ, Rhee SH. 1998. Increased antimutagenic and anticancer activities of chinese cabbage kimchi by changing kinds and levels of sub-ingredient. J Korean Soc Food Sci Nutr 27: 625-632.

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