Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Solid-Phase Extraction of Curcuminoid from Turmeric Using Physical Process Method

물리적 가공법을 이용한 강황으로부터 Curcuminoid의 고체상추출

  • Lee, Kwang-Jin (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Yang, Hye-Jin (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Jeong, Sang-Won (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine) ;
  • Ma, Jin-Yeul (KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine)
  • 이광진 (한국한의학연구원 한의신약연구그룹) ;
  • 양혜진 (한국한의학연구원 한의신약연구그룹) ;
  • 정상원 (한국한의학연구원 한의신약연구그룹) ;
  • 마진열 (한국한의학연구원 한의신약연구그룹)
  • Received : 2012.06.13
  • Accepted : 2012.09.03
  • Published : 2012.09.30
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Abstract

In order to extract the curcuminoid such as curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) in turmeric (Curcuma longa), solvent extraction methods (dipping and ultrasonic extraction method) and solid-phase extraction (SPE) were used. RP-HPLC (reverse-phase high-performance liquid chromatography) and TLC (thin-layer chromatography) were used for identification and analysis the three curcuminoid. From the experimental results, it is evident that the percentage of curcuminoid extracted from turmeric by ultrasonic extraction method was higher than dipping method. The percentage of curcumin extracted from turmeric by pure methanol was higher than any aqueous methanolic composition. Moreover, the total peak area of three curcuminoid was above 92% in RP-HPLC using solid-phase extraction. These results will form a database for investigating the constituents of natural products and the resources of pharmaceutical, nutrition, and cosmetic products.

Keywords

References

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