The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of a Novel Cucumber mosaic virus Isolated from Petunia hybrida

  • Han, Kyung-Sook (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA)) ;
  • Choi, Gug-Seoun (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA)) ;
  • Chung, Bong-Nam (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA)) ;
  • Cho, Jeom-Duk (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA)) ;
  • Cho, In-Sook (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA)) ;
  • Kim, Kee-Hong (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA)) ;
  • Kim, Su (Vegetable Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoon, Ju-Yeon (Department of Horticulture and Landscape, Seoul Women's University) ;
  • Choi, Seung-Kook (Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA))
  • Received : 2012.04.09
  • Accepted : 2012.05.16
  • Published : 2012.09.01
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Abstract

Petunia hybrida is commonly used in landscapes and interiors for its attractive flower. Virus-like foliar symptoms, including a mosaic with dark green islands surrounding the veins and chlorosis on the leaf margins, were observed on a petunia plant from Icheon, Gyeonggido, Korea. Cucumber mosaic virus (CMV) was identified in the symptomatic petunia by serological testing for the presence of CMV coat protein (CP) with a direct antibody-sandwich-enzyme-linked immunosorbent assay. An agent was mechanically transmitted to indicator plant species including Chenopodium quinoa. Examination of the inoculated plant leaves by RT-PCR analysis and electron microscopy revealed the presence of specifically amplified CP products and spherical virions of approximately 28 nm in diameter, respectively, providing confirmation of a CMV infection. Analysis of CP sequences showed that CMV petunia isolate (CMVYJC) shared 82.5-100% amino acid sequence identity with CPs of representative CMV strains. Phylogenetic analysis of CPs supports that CMV-YJC is a member of CMV subgroup IA (CMV-IA) and has biological properties of CMV-IA on host species. To our knowledge, this is the first report of CMV from P. hybrida in Korea.

Keywords

References

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