The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Effects of Cordyceps militaris(CM) on Osteoclastogenesis and Gene Expression

동충하초가 파골세포의 분화와 유전자 발현에 미치는 영향

  • Choi, Kyung-Hee (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University) ;
  • Yoo, Jeong-Eun (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University) ;
  • Hwang, Gwi-Seo (Dept. of Preventive Medicine, College of Oriental Medicine, Ga-Chon University) ;
  • Yoo, Dong-Youl (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
  • 최경희 (대전대학교 한의과대학 부인과교실) ;
  • 유정은 (대전대학교 한의과대학 부인과교실) ;
  • 황귀서 (가천대학교 한의과대학 예방의학교실) ;
  • 유동열 (대전대학교 한의과대학 부인과교실)
  • Received : 2012.07.23
  • Accepted : 2012.08.16
  • Published : 2012.08.31
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Abstract

Objectives: This study was performed to evaluate effects of Cordyceps militaris(CM) on osteoclast differentiation and its related gene expression. Methods: We used mouse myeloid cells RAW 264.7 stimulated by receptor activator of nuclear factor kappa-B ligand(RANKL) to induce osteoclast differentiation. There are four groups of which RAW 264.7 cells are not stimulated by RANKL (Normal), stimulated by RANKL without CM(Control), stimulated by RANKL with 0.1 ${\mu}g/ml$ of CM(CM 0.1), stimulated by RANKL with 1 ${\mu}g/ml$ of CM(CM 1). Osteoclastogenesis was measured by counting Tartrate-resistant acid phosphatase-positive multinucleated cells [TRAP(+) MNC]. RT-PCR was performed to evaluate the inhibitory effect of CM on gene expression(TRAP, AKT1, JNK1, NFATc1, c-Fos, MITF). Results: 1. CM decreased the number of TRAP(+) osteoclast in RANKL-stimulated RAW 264.7 cell at the concentration of 0.1 ${\mu}g/ml$ and 1 ${\mu}g/ml$. 2. CM decreased the expression of TRAP in osteoclast at the concentration of 1 ${\mu}g/ml$. 3. CM decreased the expression of AKT1, JNK1 in osteoclast at the concentration of 1 ${\mu}g/ml$. 4. CM didn't affect the expression of NFATc1, c-Fos, MITF in osteoclast. Conclusions: Cordyceps militaris has inhibitory effects on osteoclast differentiation and its related gene expression. These results suggest that Cordyceps militaris has a potential as a treatment of osteoporosis.

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References

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