DOI QR코드

DOI QR Code

Effects of Cordyceps militaris(CM) on Osteoclastogenesis and Gene Expression

동충하초가 파골세포의 분화와 유전자 발현에 미치는 영향

  • Choi, Kyung-Hee (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University) ;
  • Yoo, Jeong-Eun (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University) ;
  • Hwang, Gwi-Seo (Dept. of Preventive Medicine, College of Oriental Medicine, Ga-Chon University) ;
  • Yoo, Dong-Youl (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
  • 최경희 (대전대학교 한의과대학 부인과교실) ;
  • 유정은 (대전대학교 한의과대학 부인과교실) ;
  • 황귀서 (가천대학교 한의과대학 예방의학교실) ;
  • 유동열 (대전대학교 한의과대학 부인과교실)
  • Received : 2012.07.23
  • Accepted : 2012.08.16
  • Published : 2012.08.31

Abstract

Objectives: This study was performed to evaluate effects of Cordyceps militaris(CM) on osteoclast differentiation and its related gene expression. Methods: We used mouse myeloid cells RAW 264.7 stimulated by receptor activator of nuclear factor kappa-B ligand(RANKL) to induce osteoclast differentiation. There are four groups of which RAW 264.7 cells are not stimulated by RANKL (Normal), stimulated by RANKL without CM(Control), stimulated by RANKL with 0.1 ${\mu}g/ml$ of CM(CM 0.1), stimulated by RANKL with 1 ${\mu}g/ml$ of CM(CM 1). Osteoclastogenesis was measured by counting Tartrate-resistant acid phosphatase-positive multinucleated cells [TRAP(+) MNC]. RT-PCR was performed to evaluate the inhibitory effect of CM on gene expression(TRAP, AKT1, JNK1, NFATc1, c-Fos, MITF). Results: 1. CM decreased the number of TRAP(+) osteoclast in RANKL-stimulated RAW 264.7 cell at the concentration of 0.1 ${\mu}g/ml$ and 1 ${\mu}g/ml$. 2. CM decreased the expression of TRAP in osteoclast at the concentration of 1 ${\mu}g/ml$. 3. CM decreased the expression of AKT1, JNK1 in osteoclast at the concentration of 1 ${\mu}g/ml$. 4. CM didn't affect the expression of NFATc1, c-Fos, MITF in osteoclast. Conclusions: Cordyceps militaris has inhibitory effects on osteoclast differentiation and its related gene expression. These results suggest that Cordyceps militaris has a potential as a treatment of osteoporosis.

Keywords

References

  1. 해리슨 번역편찬위원회. Harrison's 내과학. 서울:도서출판 정담. 1997:2312-3, 2342-3.
  2. Cooper C, et al. Population-based study of survival after osteoporotic fractures. Am J Epidemiol. 1993; 137(9):1001-5. https://doi.org/10.1093/oxfordjournals.aje.a116756
  3. Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature. 2003;423(6937):337-42. https://doi.org/10.1038/nature01658
  4. Rao A, Luo C, Hogan PG. Transcription factors of the NFAT family: regulation and function. Annu Rev Immunol. 1997;15:707-47. https://doi.org/10.1146/annurev.immunol.15.1.707
  5. Rodan GA, Marfin TJ. Therapeutic approaches to bone disease. Science. 2000;289(5484):1508-14. https://doi.org/10.1126/science.289.5484.1508
  6. Nishikawa M, et al. Bisphosphonates act on osteoblastic cells and inhibit osteoclast formation in mouse marrow cultures. Bone. 1996;18(1):9-14. https://doi.org/10.1016/8756-3282(95)00426-2
  7. Kwack HB, et al. Risedronate directly inhibits osteoclast differentiation and inflammatory bone loss. Biol Pharm Bull. 2009;32(7):1193-8. https://doi.org/10.1248/bpb.32.1193
  8. Abrahamsen B. Bisphosphonate adverse effects, lessons from large databases. Curr Opin Rheumatol. 2010;22(4): 404-9. https://doi.org/10.1097/BOR.0b013e32833ad677
  9. 곽한복 등. 녹용 물 추출물의 파골세포 분화 억제효과. 동의생리병리학회지. 2008;22(4):891-5.
  10. 정연태 등. 두충의 물 추출물이 파골 세포의 분화에 미치는 영향. 동의생리병리학회지. 2009;23(3):613-8.
  11. 리 연 등. 오미자 물 추출물이 파골 세포 분화에 미치는 영향. 동의생리병리학회지. 2010;24(5):848-53.
  12. 박성태 등. 백출의 파골세포 분화에 미치는 영향. 동의생리병리학회지. 2011;25(1):109-14.
  13. 이명수 등. 파골세포 분화에 목향 물 추출물의 효과. 동의생리병리학회지. 2011;25(3):516-20.
  14. 오재민 등. 파골세포 분화에 미치는 복분자 물 추출물의 효과. 동의생리병리학회지. 2011;25(4):669-73.
  15. 전국한의과대학공동교재편찬위원회. 본초학. 서울:영림사. 2005:615-6.
  16. Ying J, et al. Icons of medical fungi from china. Beijing, China:Science press. 1987:65-85.
  17. Cho ES, Yu JH, Kim MS, Yim M. Rolipram, a phosphodiesterase 4 inhibitor, stimulates osteoclast formation by inducing TRANCE expression in mouse calvarial cells. Arch Pharm Res. 2004;27(12):1258-62. https://doi.org/10.1007/BF02975891
  18. 대한정형외과학회. 정형외과학. 서울: 대한정형외과학회. 1993:15-23, 133-5.
  19. 대한병리학회. 병리학. 서울:고문사. 1998:1073-5.
  20. 조수현. 폐경과 골다공증. 대한의학협회지. 1992;35(5):587-98.
  21. Christenson RH. Biochemical markers of bone metabolism:an overview. Clin Biochem. 1997;30(8):573-93. https://doi.org/10.1016/S0009-9120(97)00113-6
  22. Khosla S, et al. Bisphosphonate-associated osteonecrosis of the jaw: report of a task force of the American Society for Bone and mineral Research. J Bone Miner Res. 2007;22:1479-91. https://doi.org/10.1359/jbmr.0707onj
  23. Kobayasi Y. Keys to the taxa of the genera Cordyceps and Torrubiella. Trans Mycol Soc Japan. 1982;23:329-64.
  24. Su CH, Wang HH. Phytocordiceps, a new genus of the Clavicipitaceae. Mycotaxon. 1986;26:337-44.
  25. 안덕균. 한국본초도감. 서울:교학사. 2002:725.
  26. Roodman GD. Regulation of osteoclast differentiation. Ann NY Acad Sci. 2006;1068:100-9. https://doi.org/10.1196/annals.1346.013
  27. Dougall WC, et al. RANK is essential for osteoclast and lymph node development. Genes Dev. 1999;13(18): 2412-24. https://doi.org/10.1101/gad.13.18.2412
  28. Lowe C, et al. Osteopetrosis in Srcdeficient mice is due to an autonomous defect of osteoclasts. Proc Natl Acad Sci USA. 1993;90(10):4485-9. https://doi.org/10.1073/pnas.90.10.4485
  29. Takayanagi H. Osteoimmunology : shared mechanisms and crosstalk between the immune and bone systems. Nat Rev Immunol. 2007;7(4):292-304. https://doi.org/10.1038/nri2062
  30. Takayanagi H, et al. Induction and activation of the transcription factor NFATc1(NFAT2) integrate RANKL signaling in terminal differentiation of osteoclasts. Dev Cell. 2002;3(6): 889-901. https://doi.org/10.1016/S1534-5807(02)00369-6
  31. Partington GA, et al. Mitf-PU.1 interactions with the tartrate-resistant acid phosphatase gene promoter during osteoclast differentiation. Bone. 2004; 34(2):237-45. https://doi.org/10.1016/j.bone.2003.11.010
  32. Melton LJ 3rd, et al. Bone density and fracture risk in men. J Bone Miner Res. 1998;13(12):1915-23. https://doi.org/10.1359/jbmr.1998.13.12.1915

Cited by

  1. 건지황(乾地黃) 추출물이 Rat fetus 두개골로부터 분리한 조골세포에 미치는 영향 vol.26, pp.3, 2013, https://doi.org/10.15204/jkobgy.2013.26.3.033