Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Vigna angularis on Toll-like Receptor Activation and Pro-inflammatory Cytokine Production

적소두 추출물이 톨유사수용체 활성 및 염증유발 사이토카인의 생성에 미치는 영향

  • Kim, Mi-Hwa (Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeoung, See-Hwa (Natural Bio R&D Center, Sun Bio Tech) ;
  • Lee, Seung-Woong (Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hyun-Kyu (Natural Bio R&D Center, Sun Bio Tech) ;
  • Park, Chan-Sun (Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine & Research Center of Traditional Korean Medicine, Wonkwang University) ;
  • Oh, Hyun-Mee (Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology) ;
  • Rho, Mun-Chual (Bioindustrial Process Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology)
  • 김미화 (한국생명공학연구원 바이오소재연구소 생물산업공정연구센터) ;
  • 정시화 ((주)선바이오텍) ;
  • 이승웅 (한국생명공학연구원 바이오소재연구소 생물산업공정연구센터) ;
  • 김현규 ((주)선바이오텍) ;
  • 박찬선 (한국생명공학연구원 바이오소재연구소 생물산업공정연구센터) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 오현미 (한국생명공학연구원 바이오소재연구소 생물산업공정연구센터) ;
  • 노문철 (한국생명공학연구원 바이오소재연구소 생물산업공정연구센터)
  • Received : 2012.07.23
  • Accepted : 2012.08.20
  • Published : 2012.08.25
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Abstract

The mechanisms of Toll-like receptor (TLR) signaling have been the focus of extensive studies because TLRs are the target of therapeutic intervention on multiple diseases. In this study, we investigated the inhibitory potential of Vigna angularis (azuki bean) on the TLR signaling. The effect of Vigna angularis extract (JSD) on TLR activation was investigated by assessing NF-${\kappa}B$ and AP-1 inducible secreted embryonic alkaline phosphatase (SEAP) activity. JSD significantly inhibited SEAP activity induced by poly I:C (TLR3 ligand) and poly I (TLR7 ligand) in a dose-dependent manner at concentration below 100 ${\mu}g/ml$ with no sign of cytotoxicity. Pretreatment of JSD markedly suppressed mRNA expressions of pro-inflammatory cytokines and adhesive molecules such as TNF-${\alpha}$, IL-6, RANTES, MCP-1 and ICAM-1 induced by TLR ligands. It also diminished the phosphorylation of $I{\kappa}B$ kinase and $I{\kappa}B$, and followed by $I{\kappa}B$-mediated nuclear translocation of p50, p65, and phosphorylation of p38, JNK, and IRF signaling pathway. In conclusion, our results suggest that Vigna angularis has inhibitory activity on TLR-3 and -7 signaling and it can be further developed as a remedy in curing TLR-related multiple diseases.

Keywords

References

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