Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Wogonin on Hydrogen Peroxide Production in Polyinosinic-Polycytidylic acid-induced Human Neuroblastoma SH-SY5Y Cells

Wogonin이 polyinosinic-polycytidylic acid로 유발된 인간 신경모세포종 SH-SY5Y의 hydrogen peroxide 생성증가에 미치는 영향

  • Park, Wan-Su (Department of Pathology, College of Oriental Medicine, Gachon University)
  • 박완수 (가천대학교 한의과대학 병리학교실)
  • Received : 2012.05.03
  • Accepted : 2012.08.14
  • Published : 2012.08.25
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Abstract

The purpose of this study is to investigate the modulatory effect of wogonin on hydrogen peroxide production in human blastoma SH-SY5Y cells induced by the synthetic analog of double-stranded RNA [polyinosinic-polycytidylic acid]. Hydrogen peroxide production was measured by dihydrorhodamine 123 (DHR) assay. Wogonin significantly inhibited the polyinosinic-polycytidylic acid (PIC)-induced production of hydrogen peroxide for 0.5, 2, 12, 18, and 24 hr incubation at the concentrations of 10, 25, and 50 ${\mu}M$ in SH-SY5Y (P < 0.05) in dose dependent manner. These results suggest that wogonin has neuroprotective property related with its inhibition of hydrogen peroxide production in PIC-induced neuronal cells.

Keywords

References

  1. 전국한의과대학 공동교재편찬위원회. 본초학. 서울, 영림사, p 178, 1991.
  2. 이민정, 김영옥, 이강진, 유영법, 김선여, 김성수, 김호철. 황련해독탕(黃連解毒湯)의 4-VO로 유발한 흰쥐뇌허혈에 대한신경보호효과. 대한한의학회지 23(4):161-168, 2002.
  3. Comstock, A.T., Ganesan, S., Chattoraj, A., Faris, A.N., Margolis, B.L., Hershenson, M.B., Sajjan, U.S. Rhinovirus-induced barrier dysfunction in polarized airway epithelial cells is mediated by NADPH oxidase 1. J Virol. 85(13):6795-6808, 2011. https://doi.org/10.1128/JVI.02074-10
  4. 이지영, 김영진, 박완수. Emodin이 polyinosinic-polycytidylic acid로 유발된 인간 신경모세포종 SH-SY5Y의 hydrogen peroxide 생성증가에 미치는 영향. 동의생리병리학회지 25(6):1039-1043, 2011.
  5. Sun, B., Lu, C., Zhou, G.P., Xing, C.Y. Suppression of Par-4 Protects Human Renal Proximal Tubule Cells from Apoptosis Induced by Oxidative Stress. Nephron Exp Nephrol. 117(3):e53-e61, 2010.
  6. Graber, D.J., Park, P.J., Hickey, W.F., Harris, B.T. Synthetic triterpenoid CDDO derivatives modulate cytoprotective or immunological properties in astrocytes, neurons, and microglia. J Neuroimmune Pharmacol. 6(1):107-120, 2011. https://doi.org/10.1007/s11481-010-9240-9
  7. Melillo, A.A., Bakshi, C.S., Melendez, J.A. Francisella tularensis antioxidants harness reactive oxygen species to restrict macrophage signaling and cytokine production. J Biol Chem. 285(36):27553-27560, 2010. https://doi.org/10.1074/jbc.M110.144394
  8. Fila, L., Musil, J. Examination of exhaled breath condensate in cystic fibrosis. Cas Lek Cesk. 149(4):173-177, 2010.
  9. 박완수. 白蔘과 紅蔘이 포함된 理中湯의 마우스 대식세포내 hydrogen peroxide 생성에 미치는 영향. 동의생리병리학회지 25(1):78-83, 2011.
  10. 박완수. 黃芩물추출물이 마우스 대식세포의 hydrogen peroxide 생성에 미치는 영향. 대한본초학회지 26(1):53-58, 2011.
  11. 박완수. 黃芩물추출물이 LPS로 유발된 마우스 대식세포의 hydrogen peroxide 생성에 미치는 영향. 경락경혈학회지 28(3):33-41, 2011.
  12. 이지영, 박완수. 白蔘이 포함된 理中湯약침액의 LPS로 유발된 마우스 대식세포의 nitric oxide 및 hydrogen peroxide 생성에 미치는 영향. 경락경혈학회지 28(1):61-69, 2011.
  13. 이지영, 박완수. 紅蔘理中湯이 LPS로 유발된 마우스 대식세포 RAW 264.7의 nitric oxide 및 hydrogen peroxide 생성에 미치는 영향. 동의생리병리학회지 25(2):294-299, 2011.
  14. 송효인, 김광중. 베타아밀로이드로 유도된 신경세포사멸에 대한 지황(地黃) 및 지황식초(地黃食醋)의 보호효과. 동의생리병리학회지 21(1):190-198, 2007.
  15. 강태훈, 채수림, 최우영, 박 찬, 남재환, 주영란, 박근영, 조해월, 김인범. 생쥐 뇌에서 일본뇌염바이러스 감염에 의한 신경세포 자멸사 연구. Anatomy and Cell Biology. 38(3):259-264, 2005.
  16. Richardson, M.P., Ayliffe, M.J., Helbert, M., Davies, E.G. A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test. J Immunol Methods. 219(1-2):187-193, 1998. https://doi.org/10.1016/S0022-1759(98)00136-7
  17. Jirapongsananuruk, O., Malech, H.L., Kuhns, D.B., Niemela, J.E., Brown, M.R., Anderson-Cohen, M., Fleisher, T.A. Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay. J Allergy Clin Immunol. 111(2):374-379, 2003. https://doi.org/10.1067/mai.2003.58
  18. Shankaran, H., Chrisler, W.B., Sontag, R.L., Weber, T.J. Inhibition of ERK oscillations by ionizing radiation and reactive oxygen species. Mol Carcinog. 50(6):424-432, 2011. https://doi.org/10.1002/mc.20724
  19. Goodwin, A.C., Murray-Stewart, T.R., Casero, R.A. Jr. A simple assay for mammalian spermine oxidase: a polyamine catabolic enzyme implicated in drug response and disease. Methods Mol Biol. 720: 173-181, 2011.
  20. Piao, H.Z., Choi, I.Y., Park, J.S., Kim, H.S., Cheong, J.H,, Son, K.H., Jeon, S.J., Ko, K.H., Kim, W.K. Wogonin inhibits microglial cell migration via suppression of nuclear factor-kappa B activity. Int Immunopharmacol. 8(12):1658-1662, 2008. https://doi.org/10.1016/j.intimp.2008.07.018