Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Induction of Apoptosis in Glioma Cells and Upregulation of Fas Expression Using the Human Interferon-β Gene

  • Guo, Yan (Department of Neurosurgery, the 6th Hospital Affiliated to Shanghai Jiaotong University) ;
  • Wang, Gan (Department of Neurosurgery, the 6th Hospital Affiliated to Shanghai Jiaotong University) ;
  • Gao, Wen-Wei (Department of Neurosurgery, the 6th Hospital Affiliated to Shanghai Jiaotong University) ;
  • Cheng, Shi-Wen (Department of Neurosurgery, the 6th Hospital Affiliated to Shanghai Jiaotong University) ;
  • Wang, Ren (Department of Neurosurgery, the 6th Hospital Affiliated to Shanghai Jiaotong University) ;
  • Ju, Shi-Ming (Department of Neurosurgery, the 6th Hospital Affiliated to Shanghai Jiaotong University) ;
  • Cao, He-Li (Department of Neurosurgery, the 6th Hospital Affiliated to Shanghai Jiaotong University) ;
  • Tian, Heng-Li (Department of Neurosurgery, the 6th Hospital Affiliated to Shanghai Jiaotong University)
  • Published : 2012.06.30
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Abstract

We investigated whether IFN-${\beta}$ inhibits the growth of human malignant glioma and induces glioma cell apoptosis using the human IFN-${\beta}$ gene transfected into glioma cells. A eukaryonic expression vector ($pSV2IFN{\beta}$) for IFN-${\beta}$ was transfected into the glioma cell line SHG44 using liposome transfection. Stable transfection and IFN-${\beta}$ expression were confirmed using an enzyme-linked immunosorbent assay (ELISA). Cell apoptosis was also assessed by Hoechst staining and electron microscopy. In vivo experiments were used to establish a SHG44 glioma model in nude mice. Liposomes containing the human IFN-${\beta}$ gene were injected into the SHG44 glioma of nude mice to observe glioma growth and calculate tumor size. Fas expression was evaluated using immunohistochemistry. The IFN-${\beta}$ gene was successfully transfected and expressed in the SHG44 glioma cells in vitro. A significant difference in the number of apoptotic cells was observed between transfected and non-transfected cells. Glioma growth in nude mice was inhibited in vivo, with significant induction of apoptosis. Fas expression was also elevated. The IFN-${\beta}$ gene induces apoptosis in glioma cells, possibly through upregulation of Fas. The IFN-${\beta}$ gene modulation in the Fas pathway and apoptosis in glioma cells may be important for the treatment of gliomas.

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References

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