Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
권호 표지

Physiological Responses of Cirsium setidens and Pleurospermum camtschaticum under Different Shading Treatments

피음처리에 따른 고려엉겅퀴와 누룩치의 생리반응

  • Lee, Kyeong-Cheol (Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Noh, Hee-Sun (Specialty Crops Experiment Station, Gangwon-do Agricultural Research & Extension Services) ;
  • Kim, Jongh-Wan (Specialty Crops Experiment Station, Gangwon-do Agricultural Research & Extension Services) ;
  • Han, Sang-Sup (Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University)
  • 이경철 (강원대학교 산림환경과학대학 산림자원조성학과) ;
  • 노희선 (강원도 농업기술원 특화작물시험장) ;
  • 김종환 (강원도 농업기술원 특화작물시험장) ;
  • 한상섭 (강원대학교 산림환경과학대학 산림자원조성학과)
  • Received : 2012.04.10
  • Accepted : 2012.06.01
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the chlorophyll contents, photosynthetic characteristics and chlorophyll fluorescence of Cirsium setidens and Pleurospermum camtschaticum by shading treatment. Two species were grown under non-treated (full sunlight) and three different shading condition (88~93%, 65~75% and 45%~55% of full sunlight) for the experiment. Total chlorophyll content, photochemical efficiency (Fv/Fm), specific leaf area (SLA), and net apparent quantum yield were increased with elevating shading level but decreased dark respiration under the low light intensity. Therefore, light absorption and light utilization efficiency were improved under the low light intensity. 45~55% of full sunlight in C. setidens and 65~75% of full sunlight in P. camtschaticum showed best maximum photosynthetic rate, net apparent quantum yield and photochemical efficiency. On the other hand, non-treated showed lower maximum photosynthetic rate, photochemical efficiency, and chlorophyll content than treated ones. These results suggest that growth of P. camtschaticum adapted to 65~75% of full sunlight and C. setidens adapted to 45~55% of full sunlight.

피음처리에 의한 고려엉겅퀴와 누룩치의 광합성 및 생장특성을 조사하기 위하여, 차광막을 이용하여 전광처리구(0%), 약피음처리구(45~55%), 중간피음처리구(65~75%), 강피음처리구(88~92%) 설치하고 엽록소함량, 엽록소 형광반응, 광합성 반응 등을 조사하였다. 고려엉겅퀴와 누룩치 모두 피음처리 수준에 따라 엽록소 함량과 엽록소형광반응, 순양자효율 등이 증가하여 빛의 흡수와 광합성 반응에 대한 효율을 높이는 내음성 적응 반응을 나타냈으며, 엽면적 SLA 역시 증가하여 부족한 광환경에서 수광량을 늘리기 위해 엽면적은 늘어나고, 엽두께는 얇아지는 형태적인 변화가 나타났다. 특히 전광처리구에서 총 엽록소 함량, 엽록소 형광반응, 순양자효율, 기공전도도 및 기공증산속도가 비교적 낮았으며, 이는 강한 광으로 광저해 현상이 일어나 광합성 능력이 저하되는 것으로 볼 수 있다. 고려엉겅퀴의 강피음 처리구는 엽록소 함량도 높으며, 엽면적이 늘어나고 엽두께는 얇아져 광을 수집하기 위한 형태적인 변화도 나타나고 있으나 광선요구도보다 적은 광 환경에서 계속 생장함으로서 엽육내 $CO_2$를 효율적으로 소비하지 못하고, 수분이용효율 역시 감소되어 광합성 능력을 점점 상실해 가는 것으로 생각된다. 또한 고려엉겅퀴는 약광처리구(전광의 45~55%)에서최대광합성속도와 순양자효율이 가장 높고, 기공개폐 반응과 광화학반응 효율이 비교적 높았으며, 누룩치는 처리구간의 차이는 크지 않지만, 중간피음처리구에서 최대광합성속도, 순양자수율, 기공개폐반응 및광화학효율이 가장 높은 것으로 나타났다. 따라서 적절한 피음처리를 통해 건전한 생육을 유도하기 위해서는 고려엉겅퀴의 경우 전광을 약 45~55% 차단하고, 누룩치는 약 65~75% 차단시킨 광환경이 효과적이라 생각된다.

Keywords

References

  1. Andrew, J.J. and J.D. William. 1998. The coupled response of stomatal conductance to photosynthesis and transpiration. J. Exp. Bot. 49:399-406.
  2. Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts, polyphenoloxidase in Beta vulgaris. J. Plant Physiol. 24:1-15. https://doi.org/10.1104/pp.24.1.1
  3. Caemmerer, S. and G.D. Farquhar. 1981. Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta. 153:376-387. https://doi.org/10.1007/BF00384257
  4. Cho, M.S., K.W. Kwon, G.N. Kim, and S.Y. Woo. 2008. Chlorophyll contents and growth performances of the five deciduous hardwood species growing under different shade treatments. Kor. J. Agr. For. Meteorol. 10:149-157 (in Korean). https://doi.org/10.5532/KJAFM.2008.10.4.149
  5. Cho, S.D., D.H. Choi, and K.H. Kim. 2004. A study on quality characteristics of Pleurospermum kamtschaticum as a functional food resource. J. Kor. Soc. Food Sci. Nut. 33:1212-1217 (in Korean). https://doi.org/10.3746/jkfn.2004.33.7.1212
  6. Choi, Y.B. and J.H. Kim. 1995. Change in needle chlorophyll fluorescence of Pinus densiflora and Pinus thunbergii treated with artificial acid rain. J. Kor. For. Soc. 84:97-102 (in Korean).
  7. Chung, M.S. and M.S. Lee. 1998. Analysis of volatile flavor component of Pleurospermum kamtschaticum. Kor. J. Food Cook. Sci. 14:541-547 (in Korean).
  8. Demmig, B. and O. Bjorkman. 1987. Comparison of the effect of excessive light on chlorophyll fluorescence (77 K) and photon yield of $O_2$ evolution in leaves of higher plants. Planta. 171:171-184. https://doi.org/10.1007/BF00391092
  9. Evans, J.R. 1987. The dependence of quantum yield on wavelength and growth irradiance. Aust. J. Plant Physiol. 14:69-79. https://doi.org/10.1071/PP9870069
  10. Han, S.S., K.C. Lee, and S.R. Jeon. 2010. Studies on the agroforestry methods of wild edible greens(IV) water relations parameters of three Ligularia species leaves obtained from P-V curves. J. Kor. For. Soc. 99:131-135 (in Korean).
  11. Hansen, U., B. Fiedler, and B. Rank. 2002. Variation of pigment composition and antioxidative systems along the canopy light gradient in a mixed beech/oak forest: a comparative study on deciduous tree species differing in shade tolerance. Trees. 16:354-364. https://doi.org/10.1007/s00468-002-0163-9
  12. Hernandez, J.A., E. Olmos, F.J. Corpas, F. Sevilla, and L.A. del Rio. 1995. Salt-induced oxidative stress in chloroplasts of pea plant. Plant Sci. 105:151-167. https://doi.org/10.1016/0168-9452(94)04047-8
  13. Hiscox, J.D. and G.F. Israelstam, 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot. 57:1332-1334. https://doi.org/10.1139/b79-163
  14. Hong, D.K. 1997. Studies on the cultivation of Pleurospermum kamtschaticum Hoff. to high land area. M. Sc. thesis. Univ. Kangwon. Chun-Cheon. Korea. p. 51 (in Korean).
  15. Je, S.M., S.G. Son, S.Y. Woo, K.O. Byun, and C.S. Kim. 2006. Photosynthesis and chlorophyll contents of Chloranthus glaber under different shading treatments. Kor. J. Agr. For. Meteorol. 8:54-60 (in Korean).
  16. Jeong, H.H. and K.S. Kim. 1999. Effects of shading on the growth of Hedera rhombea bean and Pachysandra terminalis Sieb. et Zucc. Kor. J. Hort. Sci. Technol. 17:29-32 (in Korean).
  17. Jung, M.J., S.I. Heo, and M.H. Wang. 2008. Rat lens aldose reductase inhibitory of Taraxacum mongolicum and two Crisium species. J. Kor. Soc. App. Bio. Chem. 51:302-306 (in Korean). https://doi.org/10.3839/jabc.2008.047
  18. Kim, G.N., M.S. Cho, and S.W. Lee. 2010. Physiological responses of the three deciduous hardwood seedlings growing under different shade treatment regimes. J. Bio-Env. Con. 19:36-48 (in Korean).
  19. Kim, K.D., E.H. Lee, W.B. Kim, J.G. Lee, D.L. Yoo, Y.S. Kwon, J.N. Lee, S.W. Jang, and S.C. Hong. 2010. Year-round production of fresh leaves of narrowhead goldenray 'Ligularia stenocephala' by using stored rootstocks in sequential highland-lowland cultivation. J. Bio-Env. Con. 19:324-332 (in Korean).
  20. Kim, S.H., J.H. Saung, Y.K. Kim, and P.G. Kim. 2008. Photosynthetic responses of four oak species to changes in light environment. Kor. J. Agr. For. Meteorol. 10: 141-148 (in Korean). https://doi.org/10.5532/KJAFM.2008.10.4.141
  21. Kim, P.G. and E.J. Lee. 2001a. Ecophysiology of photosynthesis 1: effects of light intensity and intercellular $CO_2$ pressure on photosynthesis. Kor. J. Agr. For. Meteorol. 3:126-133 (in Korean).
  22. Kim, P.G. and E.J. Lee. 2001b. Ecophysiology of photosynthesis 2: adaptation of the photosynthetic apparatus to changing environment. Kor. J. Agr. For. Meteorol. 3:171-176 (in Korean).
  23. Kitao, M., H. Utsugi, S. Kuramoto, R. Tabuchi, K. Fujimoto, and S. Lihpai. 2003. Light-dependent photosynthetic characteristics indicated by chlorophyll fluorescence in five mangrove species native to Pohnpei Island, Micronesia. Physiologia Plantarum 117:376-382. https://doi.org/10.1034/j.1399-3054.2003.00042.x
  24. Kok, B. 1948. A critical consideration of the quantum yield of chlorella photosynthesis. Enzymologia Hague. 13:1-56.
  25. Kume, A. and Y. Ino. 1993. Comparison of ecolphysiological response to heavy snow in two varieties of Acuba japonica with different areas of distribution. Ecol. Res. 8:111-121. https://doi.org/10.1007/BF02348523
  26. Kyparissis, A., P. Drilias, and Y. Manetas. 2000. Seasonal fluctuations in photoprotective (xanthophylls cycle) and photoselective (chlorophylls) capacity in eight Mediterranean plant species belonging to two different growth forms. Aust. J. Plant Physiol. 27:265-272.
  27. Kwon, K.W., G.N. Kim, and M.S. Cho, 2009. Physiology responses of the three wild vegetable under different shading treatment. J. Kor. For. Soc. 98:106-114 (in Korean).
  28. Kwon, Y.M., S.C. Ko, J.C. Kim, B.Y. Moon, M.C. Park, H.B. Park, I.H. Park, Y.S. Lee, I.H. Lee, J.S. Lee, J.B. Lee, C.H. Lee, B.U. Jeon, S.H. Jo, and J.B. Hong. 2003. Plant Physiology. Academybook. Seoul. Korea. p. 429. (in Korean).
  29. Lavorel, J. and A.L. Etienne, 1977. In vivo chlorophyll fluorescence, pp. 203-268. In: J. Barber (ed.). Primary processes of photosynthesis. Elsevier Scientific. New York. USA.
  30. Lee, S.H., Y.S. Jin, S.I. Heo, T.H. Shin, J.H. Sa, D.S. Choi, and M.H. Wang. 2006. Composition analysis and antioxdative activity from different organs of Cirsium setidens Nakai. Kor. J. Food Sci. Tech. 38:571- 576 (in Korean).
  31. Lim, J.H., S.Y. Woo, M.J. Kwon, J.H. Chun, and J.H. Shin. 2006. Photosynthetic capacity and water use efficiency under different temperature regimes on healty and declining korean Fir in Mt. Halla. J. Kor. For. Soc. 95:705-710 (in Korean).
  32. Nam, Y.K. and J.A. Baik. 2005. Status of research and possibility of development about endemic wild vegetables in korea. Korean Society for People, Plant, and Environment. 8:1-10 (in Korean).
  33. Rascher, U., M. Liebig, and U. Lttge. 2000. Evaluation of instant light-response curves of chlorophyll fluorescence parameters obtained with a portable chlorophyll fluorometer on site in the field. Plant Cell and Environment. 23:1397-1405. https://doi.org/10.1046/j.1365-3040.2000.00650.x
  34. Sharp, R.E., M.A. Matthews, and J.S. Boyer. 1984. Kok effect and the quantum yield of photosynthesis. J. Plant Physiol. 75:95-101. https://doi.org/10.1104/pp.75.1.95
  35. Sim, J.S. and S.S. Han. 2003. Ecophysiological characteristics of deciduous oak species(III) photosynthetic responses of leaves to change of light intensity. J. Kor. For. Soc. 92:208-214 (in Korean).
  36. Suh, J.T., S.Y. Ryu, W.B. Kim, K.S. Choi, and B.H. Kim. 1996. Improvement of germination rate by low temperature and development of effective shading cultivation of Cirsium setidens under rain shelter in highland. Kor. J. Plant Res. 9:151-156 (in Korean).
  37. Sung, J.H., M.J. Sun, S.H. Kim, and Y.K. Kim. 2009. Effect of calcium chloride $(CaCl_2)$ on the characteristics of photosynthetic apparatus, stomatal conductance, and fluorescence image of the leaves of Cornus kousa. Kor. J. Agr. For. Meteorol. 11:143-150 (in Korean). https://doi.org/10.5532/KJAFM.2009.11.4.143
  38. Takemura, T., N. Hangata, K. Sugihara, S. Baba, I. Karube, and Z. Dubinsky. 2000. Physiological and biochemical responses to salt stress in the mangrove, Bruguiera gymnorrhiza. Aquatic Botany. 68:15-28. https://doi.org/10.1016/S0304-3770(00)00106-6
  39. Valladares, F., J.M. Chico, I. Aranda, L. Balaguer, P. Dizengremel, E. Manrique, and E. Dreyer. 2002. The greater seedling high-light tolerance of Quercus robur over Fagus sylvatica is linkes to a greater physiological plasticity. Trees. 16:395-403.