Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Inhibition of ENNG-Induced Pyloric Stomach and Small Intestinal Carcinogenesis in Mice by High Temperature- and Pressure-Treated Garlic

  • Kaneko, Takaaki (Fujita Memorial Nanakuri Institute, School of Medicine, Fujita Health University) ;
  • Shimpo, Kan (Fujita Memorial Nanakuri Institute, School of Medicine, Fujita Health University) ;
  • Chihara, Takeshi (Fujita Memorial Nanakuri Institute, School of Medicine, Fujita Health University) ;
  • Beppu, Hidehiko (Fujita Memorial Nanakuri Institute, School of Medicine, Fujita Health University) ;
  • Tomatsu, Akiko (Fujita Memorial Nanakuri Institute, School of Medicine, Fujita Health University) ;
  • Shinzato, Masanori (School of Health Sciences, School of Medicine, Fujita Health University) ;
  • Yanagida, Takamasa (School of Health Sciences, School of Medicine, Fujita Health University) ;
  • Ieike, Tsutomu (School of Health Sciences, School of Medicine, Fujita Health University) ;
  • Sonoda, Shigeru (Fujita Memorial Nanakuri Institute, School of Medicine, Fujita Health University) ;
  • Futamura, Akihiko (Department of Surgery & Palliative Medicine, School of Medicine, Fujita Health University) ;
  • Ito, Akihiro (Department of Surgery & Palliative Medicine, School of Medicine, Fujita Health University) ;
  • Higashiguchi, Takashi (Department of Surgery & Palliative Medicine, School of Medicine, Fujita Health University)
  • Published : 2012.05.30
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Abstract

High temperature- and pressure-treated garlic (HTPG) has been shown to have enhanced antioxidative activity and polyphenol contents. Previously, we reported that HTPG inhibited 1,2-dimethylhydrazine-induced mucin depleted foci (premalignant lesions) and $O^6$-methylguanine DNA adduct formation in the rat colorectum. In the present study, we investigated the modifying effects of HTPG on N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG)-induced pyloric stomach and small intestinal carcinogenesis in mice. Male C57BL/6 mice were given ENNG (100 mg/l) in drinking water for the first 4 weeks, then a basal diet or diet containing 2% or 5% HTPG for 30 weeks. The incidence and multiplicity of pyloric stomach and small intestinal (duodenal and jejunal) tumors in the 2% HTPG group (but not in the 5% HTPG group) were significantly lower than those in the control group. Cell proliferation of normal-appearing duodenal mucosa was assessed by MIB-5 immunohistochemistry and shown to be significantly lower with 2% HTPG (but again not 5% HTPG) than in controls. These results in dicate that HTPG, at 2% in the diet, inhibited ENNG-induced pyloric stomach and small intestinal (especially duodenal) tumorigenesis in mice, associated with suppression of cell proliferation.

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References

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