한국축산식품학회지 (Food Science of Animal Resources)

  • 제32권4호
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  • Pages.434-440
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  • 2012
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  • 2636-0772(pISSN)
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  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
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Probiotics Inhibit Lipopolysaccharide-Induced Interleukin-8 Secretion from Intestinal Epithelial Cells

  • Oh, Hyun-Wook (Division of Food Bioscience and Technology, Korea University) ;
  • Jeun, Gi-Hoon (Division of Food Bioscience and Technology, Korea University) ;
  • Lee, Jin (Division of Food Bioscience and Technology, Korea University) ;
  • Chun, Tae-Hoon (Division of Biotechnology, Korea University) ;
  • Kim, Sae-Hun (Division of Food Bioscience and Technology, Korea University)
  • 투고 : 2012.01.26
  • 심사 : 2012.07.24
  • 발행 : 2012.08.31
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초록

It has been suggested that probiotics could be useful for the prevention of symptomatic relapse in patients with inflammatory bowel disease (IBD). Interleukin (IL)-8 has been well recognized as one of the pro-inflammatory cytokines that could trigger inflammation and epithelial barrier dysfunction. In this study, the anti-inflammatory effects of probiotics were investigated using a human epithelial cell line (HT-29). Probiotics from infant feces and kimchi were tested for their cytotoxicity and effects on adhesion to epithelial cells. The present results show that seven strains could form 70 % adhesion on HT-29. The probiotics used in this study did not affect HT-29 cell viability. To screen anti-inflammatory lactic acid bacteria, HT-29 cells were pretreated with live and heat-killed probiotics, and lipopolysaccharide (LPS) ($1{\mu}g/mL$) was then added to stimulate the cells. The cell culture supernatant was then used to measure IL-8 secretion by ELISA, and the cell pellet was used to determine IL-8 and toll-like receptor (TLR-4) mRNA expression levels by RT-PCR. Some probiotics (KJP421, KDK411, SRK414, E4191, KY21, and KY210) exhibited anti-inflammatory effects through the repression of IL-8 secretion from HT-29 cells. In particular, Lactobacillus salivarius E4191, originating from Egyptian infant feces, not only decreased IL-8 mRNA expression, but also decreased TLR-4 expression. These results indicate that Lactobacillus salivarius E4191 may have a protective effect in intestinal epithelial cells.

키워드

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피인용 문헌

  1. Paraprobiotics: Evidences on their ability to modify biological responses, inactivation methods and perspectives on their application in foods vol.58, 2016, https://doi.org/10.1016/j.tifs.2016.09.011
  2. Immunomodulatory activity of exopolysaccharide producing Leuconostoc citreum strain isolated from Pico cheese vol.33, 2017, https://doi.org/10.1016/j.jff.2017.03.054
  3. The anti‐inflammatory and anti‐oxidative potential of synbiotics in two independent cell lines vol.74, pp.3, 2012, https://doi.org/10.1111/1471-0307.12777
  4. Ligilactobacillus salivarius functionalities, applications, and manufacturing challenges vol.106, pp.1, 2012, https://doi.org/10.1007/s00253-021-11694-0