Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Probiotics Inhibit Lipopolysaccharide-Induced Interleukin-8 Secretion from Intestinal Epithelial Cells

  • Oh, Hyun-Wook (Division of Food Bioscience and Technology, Korea University) ;
  • Jeun, Gi-Hoon (Division of Food Bioscience and Technology, Korea University) ;
  • Lee, Jin (Division of Food Bioscience and Technology, Korea University) ;
  • Chun, Tae-Hoon (Division of Biotechnology, Korea University) ;
  • Kim, Sae-Hun (Division of Food Bioscience and Technology, Korea University)
  • Received : 2012.01.26
  • Accepted : 2012.07.24
  • Published : 2012.08.31
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Abstract

It has been suggested that probiotics could be useful for the prevention of symptomatic relapse in patients with inflammatory bowel disease (IBD). Interleukin (IL)-8 has been well recognized as one of the pro-inflammatory cytokines that could trigger inflammation and epithelial barrier dysfunction. In this study, the anti-inflammatory effects of probiotics were investigated using a human epithelial cell line (HT-29). Probiotics from infant feces and kimchi were tested for their cytotoxicity and effects on adhesion to epithelial cells. The present results show that seven strains could form 70 % adhesion on HT-29. The probiotics used in this study did not affect HT-29 cell viability. To screen anti-inflammatory lactic acid bacteria, HT-29 cells were pretreated with live and heat-killed probiotics, and lipopolysaccharide (LPS) ($1{\mu}g/mL$) was then added to stimulate the cells. The cell culture supernatant was then used to measure IL-8 secretion by ELISA, and the cell pellet was used to determine IL-8 and toll-like receptor (TLR-4) mRNA expression levels by RT-PCR. Some probiotics (KJP421, KDK411, SRK414, E4191, KY21, and KY210) exhibited anti-inflammatory effects through the repression of IL-8 secretion from HT-29 cells. In particular, Lactobacillus salivarius E4191, originating from Egyptian infant feces, not only decreased IL-8 mRNA expression, but also decreased TLR-4 expression. These results indicate that Lactobacillus salivarius E4191 may have a protective effect in intestinal epithelial cells.

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References

  1. Cario, E. and Pldosky, D. K. (2000) Differential alteration in intestinal epithelial cell expression of toll-like receptor 3 (TLR-3) and TLR-4 in inflammatory bowel disease. Infect. Immun. 68, 1010-1017.
  2. Chuang, L., Wu, K. G., Pai, C., Hsieh, P. S., Tsai, J. J., Yen, J. H., and Lin, M. Y. (2007) Heat-killed cells of lactobacilli skew the immune response toward T helper 1 polarization in mouse splenocytes and dendritic cell-treated T cells. J. Agric. Food Chem. 55, 11080-11086. https://doi.org/10.1021/jf071786o
  3. Chung, K. T., Fulk, G. E., and Slein, M. W. (1975) Tryptophanase of fecal flora as a possible factor in the etiology of colon cancer. J. Natl. Cancer Inst. 54, 1073-1078.
  4. Dunne, C., O'Mahony, L., Murphy, L., Thornton, G., Morrisscy, D., O'Halloran, S., Feeney, M., Flynn, S., and Fitzgerald, G. (2001) In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am. J. Clin. Nutr. 73, 386s-392s.
  5. Fedorak, R. N. and Madsen, K. L. (2004) Probiotics and the management of inflammatory bowel disease. Inflamm. Bowel. Dis. 10, 286-299. https://doi.org/10.1097/00054725-200405000-00018
  6. Gewirtz, A. T., McCormick, B., Neish, A. S., Petasis, N. A., Gronert, K., Serhan, C. N., and Madara, J. L. (1998) Pathogen-induced chemokine secretion from model intestinal epithelium is inhibited by lipoxin A4 analogs. J. Clin. Invest. 101, 1860-1869. https://doi.org/10.1172/JCI1339
  7. Harada, A., Sekido, N., Akahoshi, T., Wada, T., Mukaida, N., and Matsushima, K. (1994) Essential involvement of interleukin-8 (IL-8) in acute inflammation. J. Leukoc. Biol. 56, 559-564.
  8. Ingalls, R. R., Heine, H., Lien, E., Yoshimura, A., and Glenbock, D. (1999) Lipopolysaccharide recognition, CD14, and lipopolysaccharide receptors. Infect. Dis. Clin. North Am. 13, 341-353. https://doi.org/10.1016/S0891-5520(05)70078-7
  9. Jung, H. C., Eckmann, I., Yang, S. K., Panja, A., Fierer, J., Morzycka-Worblewska, E., and Kagnoff, M. F. (1995) A distinct array of pro-inflammatory cytokines is expressed in human colon epithelia cells in response to bacterial invasion. J. Clin. Invest. 95, 55-65. https://doi.org/10.1172/JCI117676
  10. Kucharzik, T., Maaser, C., Lugering, A., Kagnoff, M., Mayer, L., Targan, S., and Domschke, W. (2006) Recent understanding of IBD pathogenesis: Implications for future therapies. Inflamm. Bowel. Dis. 12, 1068-1083. https://doi.org/10.1097/01.mib.0000235827.21778.d5
  11. Mankertz, J. and Schulzke, J. D. (2007) Altered permeability in inflammatory bowel disease: Pathophysiology and clinical implications. Curr. Opin. Gastroenterol. 23, 379-383. https://doi.org/10.1097/MOG.0b013e32816aa392
  12. Martich, G. D., Danner, R. L., Ceska, M., and Suffredini, A. F. (1991) Detection of interleukin 8 and tumor necrosis factor in normal humans after intravenous endotoxin: The effect of anti-inflammatory agents. J. Exp. Med. 173, 1021-1024. https://doi.org/10.1084/jem.173.4.1021
  13. Mennigen, R., Nolte, K., Rijcken, E., Utech, M., Loeffler, B., Senninger, N., and Bruewer, M. (2009) Probiotic mixture VSL#3 protects the epithelial barrier by maintaining tight junction protein expression and preventing apoptosis in a murine model of colitis. Am. Physiological. Soc. 296, 1140-1149. https://doi.org/10.1152/ajpcell.00071.2009
  14. Petrof, E. O., Kojima, K., Ropeleski, M. J., Musch, M. W., Tao, Y., De Simone, C.D., and Chang, E. B. (2004) Probiotics inhibit nuclear factor-kappaB and induce heat shock proteins in colonic epithelial cells through proteasome inhibition. Gastroenterol. 127, 1474-1487. https://doi.org/10.1053/j.gastro.2004.09.001
  15. Pochard, P., Gosset, P., Grangette, C., Andre, C., Tonnel, A. B., Pestel, J., and Mercenier, A. (2002) Lactic acid bacteria inhibit TH2 cytokine production by mononuclear cells from allergic patients. J. Allergy Clin. Immunol. 110, 617-623. https://doi.org/10.1067/mai.2002.128528
  16. Rhodes, J. M., Gallimore, R., and Elias, E. (1985) Fecal mucus degrading glycosidase in ulcerative colitis and Crohn's disease. Gut. 26, 761-765. https://doi.org/10.1136/gut.26.8.761
  17. Salminen, S., Ouwehand, A. C., and Isolauri, E. (1998) Clinical applications of probiotic bacteria. Int. Dariy. J. 8, 563-572. https://doi.org/10.1016/S0958-6946(98)00077-6
  18. Sashihara, T., Sueki, N., and Ikegami, S. (2006) An analysis of the effectiveness of heat-killed lactic acid bacteria in alleviating allergic diseases. J. Dairy Sci. 89, 2846-2855. https://doi.org/10.3168/jds.S0022-0302(06)72557-7
  19. Shanahan, F. and Bernstein, C. N. (2009) The evolving epidemiology of inflammatory bowel disease. Curr. Opin. Gastroenterol. 25, 301-305. https://doi.org/10.1097/MOG.0b013e32832b12ef
  20. Shanahan, F. (2001) Immunodiagnostics, immunotherapeutics, and ecotherapies. Gastroenterol. 120, 622-635. https://doi.org/10.1053/gast.2001.22122
  21. Turner, J. R. (2006) Molecular basis of epithelial barrier regulation from basic mechanisms to clinical application. Am. J. Pathol. 169, 1901-1909. https://doi.org/10.2353/ajpath.2006.060681
  22. Yasumoto, K., Okamoto, S., Mukaida, N., Murakami, S., Mai, M., and Matsushima, K. (1992) Tumor necrosis factor alpha and interferon gamma synergistically induce interleukin 8 production in a human gastric cancer cell line through acting concurrently on AP-1 and NF-kappaB-like binding sites of the interleukin 8 gene. J. Biol. Chem. 267, 22506-22511.

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