Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Tumeric (Curcuma longa) on Bile Acid and UDP-glucuronyl Transferase Activity in Rats Fed a High-fat and -cholesterol Diet

울금(Curcuma longa L.)이 고지방·고콜레스테롤 식이 흰쥐에서의 담즙산 및 UDP-glucuronyl transferase 활성에 미치는 영향

  • Kim, Min-Sun (Department of Food Science, International University of Korea) ;
  • Chun, Sung-Sik (Department of Food Science, International University of Korea) ;
  • Kim, Sang-Hun (Department of Microbiology and Immunology, Pusan National University School of Medicine) ;
  • Choi, Jung-Hwa (Department of Food and Nutrition, International University of Korea)
  • 김민선 (한국국제대학교 식품과학과) ;
  • 전성식 (한국국제대학교 식품과학과) ;
  • 김상헌 (부산대학교 의학전문대학원 미생물학 및 면역학 교실) ;
  • 최정화 (한국국제대학교 식품영양학과)
  • Received : 2012.06.08
  • Accepted : 2012.07.17
  • Published : 2012.08.30
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Abstract

The current study examined the effect of turmeric powder on bile acid and UDP-glucuronyl transferase activity in rats fed a high-fat and -cholesterol diet. Sprague-Dawley male rats weighing $120{\pm}10$ g were randomly assigned to a normal diet group (N group) and a high-fat and -cholesterol diet group (HF group), which was further divided into a high-fat and high-cholesterol with a 2.5% tumeric powder supplement group (TPA group) and 5% turmeric powder-supplemented group (TPB group). Body weight gain and food efficiency ratio were significantly increased in the N group as compared to the HF group, but they were significantly decreased in turmeric-supplemented groups as compared to the HF group. The total serum cholesterol and TG contents of the turmeric-supplemented groups were decreased as compared to those of the HF group. Especially, the TPB group was significantly decreased as compared to the HF group. The serum LDL-cholesterol and AI of the turmeric-supplemented groups were decreased as compared to the HF group. The hepatic triglyceride contents of all groups supplemented with the tumeric powder were significantly decreased as compared to the HF group. The hepatic UDP-glucuronyl transferase activity of the turmeric-supplemented groups was increased as compared to the HF group. In particular, the TPB group was significantly increased as compared to the HF group. The serum total bile acid contents of the turmeric-supplemented groups were increased as compared to the HF group. These results suggest that tumeric has powerful health benefits that are created via UDP-glucuronyl transferase activity, bile acid, and lipid metabolism.

본 연구는 울금 분말을 고지방 고콜레스테롤 쥐에 농도별로 2.5% (TPA group), 5% (TPB gorup)의 수준으로 공급하였을 시 담즙산 및 UDP-glucuronyl transferase (UDP-GT) 활성에 미치는 영향을 관찰하였다. 체중증가량은 정상군에 비해 고지방 고콜레스테롤 군에서 유의적으로 증가하였으나, 울금 분말을 공급한 군에서는 체중의 감소가 나타났다. 식이섭취량 및 식히효율에서는 정상군에 비해 고지방 고콜레스테롤 군에서 증가하였다. 총콜레스테롤 함량과 중성지질 함량은 정상군에 비해 고지방 고콜레스테롤 군에서 유의적으로 증가하였다. 혈중의 HDL 콜레스테롤은 정상군에 비해 고지방 고콜레스테롤 군에서 유의적으로 감소하였고, 울금 분말 공급군에서는 고지방 고콜레스테롤 군에 비해 유의적으로 증가하는 경향을 보였다. 반면, LDL 콜레스테롤 및 동맥경화지수(AI)에서는 고지방 고콜레스테롤에서 정상군에 비해 유의적으로 증가하였다. 간조직의 총 지질함량을 관찰한 결과 중성지질의 함량 및 총 콜레스테롤 함량은 정상군에 비해 고지방 고콜레스테롤 군에서 유의적으로 증가하였으며, 고지방 고콜레스테롤 군에 비해 울금 분말 공급군은 유의적으로 감소하는 경향을 나타내었다. 분변의 총 콜레스테롤 함량 및 중성지방 함량은 울금 분말을 공급한 군에 비해 고지방 고콜레스테롤군에 비해 다소 증가되었다. 혈청 중의 담즙산 농도는 고지방 고콜레스테롤 군에 비해 울금 분말을 공급한 TPA, TPB 군에서 유의적으로 증가 하였으며, UDP-GT활성을 관찰한 결과는 울금 분말 5%를 공급한 TPB 군은 고지방 고콜레스테롤 군에 비해 유의적으로 증가되었을 뿐만 아니라 가장 높은 활성을 나타내었다. 따라서 본 연구의 결과로 미루어 울금은 담즙산 및 UDP-GT의 활성을 높여줌으로써 지질대사의 개선작용을 지니고 있음을 확인할 수 있었다.

Keywords

References

  1. Arafa, H. M. 2005. Curcumin attenuates diet-induced hyper- cholesterolemia in rats. Med. Sci. Monit. 11, 28-234.
  2. Bandary, S. and Reddy, B. S. 1981. Diet and excretion of bile acids. Cancer Res. 41, 3766-3768.
  3. Byun, J. H. 1995. The goals and strategy for health promotion. Korean Institute for Health and Social Affairs.
  4. Cha, J. T., Cho, Y. S. and Kim, D. J. 2001. Effect of chicory extract on the lipid metabolism in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 30, 1220-1226.
  5. Cha, J. Y., Jeon, B. S., Park, J. W., Kim, B. K., Jeong, C. Y., Ryu, J. S., Choi, C. K. and Cho, Y. S. 2004. Hypocholesterolemic effect of yogurt supplemented salicornia herbacea extract in cholesterol-fed rat. J. Life Sci. 5, 747-751.
  6. Choi, S. W., Yang, J. S., Lee, H. S., Kim, K. S., Bai, D. H. and Yu, J. H. 2003. Characterization of squalene synthase inhibitor isolatied from Curcuma longa. Korea J. Food Sci. Technol. 35, 297-301.
  7. Fiordaliso, M., Kor, N., Kesager, K. P., Goethals, F., Deboyser, K., Roberfoid, M. and Kelzenne, N. 1995. Kietary oligofructose lowers triglycerides, phospholipids and cholesterol in serum and very low density lipoproteins of rats. Lipids 30, 163-167. https://doi.org/10.1007/BF02538270
  8. Folch, J. M., Lees, M. and Stanley, G. H. S. 1957. A simple method for the isolation and purification of total lipid from animal tissue. J. Biol. Chem. 26, 497-509.
  9. Friedewald, W. T., Levy, R. I. and Fredrickson, D. S. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18, 499-502.
  10. Gordon, T., Castelli, W. and Dawber, T. 1981. Lipoprotein, cardiovascular disease and death, the Framingham study. Arch. Inter. Med. 14, 1128-1135.
  11. Govinidaragan, V. S. 1980. Tumeric-chemistry, technology and quality, CRC Crit. Rev. Food Sci. Nutr. 12, 199-201. https://doi.org/10.1080/10408398009527278
  12. Jang, I. W. 2008. Physiological activites of fermented turmeric. MS. Thesis, Woosuk University.
  13. Kang, J. K., Kang, H. Y., Seo, J. H., Kim, S. O. and Choi, J. H. 2009. Effects of fermented turmeric (Curcuma longa) by Bacillus natto supplementation on liver function and serum lipid parameters in mice. J. Korean Soc. Food Sci. Nutr. 38, 430-435. https://doi.org/10.3746/jkfn.2009.38.4.430
  14. Kesaniemi, Y. A. and Miettinen, T. A. 1987. Cholesterol absorption efficiency regulates plasma cholesterol level in the finnish population. Eur. J. Clin. Invest. 17, 391-395. https://doi.org/10.1111/j.1365-2362.1987.tb01132.x
  15. Kim, S. O., Rhee, I. L. and Rhee, S. J. 2001. Effectsof dietary xylooligosaccharide on hepatic UDP-glucuronyl transferase activity and compositions of fecal sterols in rat high cholesterol diets. J. Korean Soc. Food Sci. Nutr. 30, 1197-1203.
  16. Kim, T. H., Son, Y. K., Hwang, K. H. and Kim, M. H. 2008. Effect of Angelica keiskei Koidzumi and turmeric extract supplementation on serum lipid parameters in hytercholesterolemic kiet or p-407-induced hyterlipidemic rats. J. Nutr. 37, 708-713.
  17. Oh, S. W., Lee, C. U. and Koh, J. B. 2004. Effects of Agaricus blazei Murill on lipid metabolism in rats fed high fat diet. J. Korean Soc. Food Sci. Nutr. 33, 821-826. https://doi.org/10.3746/jkfn.2004.33.5.821
  18. Park, Y. H., Lee, J. S. and Lee, Y. J. 1993. Distribution of serum lipid by age and the relation of serum lipid to degree of obesity and blood pressure in Korean adults. Kor. J. Lipid. 3, 165-180.
  19. Paul, J. A. 1979. Lipophilicity of acceptor substrate as a factor in late foetal rat liver microsomeal UDP-glucuronosyltransferase activity. Biochem. Pharmacolog. 29, 999-1006.
  20. Pearson, S., Stern, S. and McGaracd, T. H. 1953. A rapid accurate method for the determination of total cholesterol in serum. Anal. Chem. 25, 813-814. https://doi.org/10.1021/ac60077a041
  21. Rao, D. S., Sekhara, N. C., Satyannarayana, M. N. and Srinivasan, M. 1970. Effect of curcumin on serum and liver cholesterol levels in the rat. J. Nutr. 100, 1307-1900.
  22. Reinke, L. A., Moyer, M. J. and Notley, K. A. 1986. Diminished rats of glucuronidation and sulfation in perfused rat liver after chronic ethanol administration. Biochem. Pharmacol. 35, 439-447. https://doi.org/10.1016/0006-2952(86)90217-0
  23. Sale, F. D., Marchesini, S., Fishman, P. H. and Berra, B. 1984. A sensitive enzymatic assay for determination of cholesterol in lipid extracts. p 347-350, Academic Press Inc, New York.
  24. Scragg, I., Celier, C. and Burchell, B. 1985. Congenital jaundice in rats due to the absence of hepatic bilirubin UDP-glucuronyltransferase enzyme protein. FEBS Lett. 183, 37-42. https://doi.org/10.1016/0014-5793(85)80949-2
  25. Singhal, A. K., Finver-Sadowsky, J., Mcherry, C. K. and Mosbach, E. H. 1983. Effect of cholesterol and bile acids on the regulation of cholesterol metabolism in hamster. Biochem. Biophys. Acta. 752, 211-222.
  26. Sin, M. K. and Han, S. H. 2001. Effects of methanol extracts from phaseolus vulgaris on serum lipid concentrations in rats fed high fat and cholesterol diet. Korean J. Food Sci. Technol. 1, 113-116.
  27. Song, W. Y., Kim, Y. N., Chun, S. S., Ku, K. H. and Choi, J. H. 2011. Effect of ethanol extracts from red pepper (Capsicum annuum L.) seeds on cholesterol adsorption capacity and UDP-glucuronyl transferase acticity. Korean J. Life Sci. Nutr. 21, 829-837.
  28. Song, W. Y., Sung, B. H., Kang, S. K. and Choi, J. H. 2010. Effect of water extracts from phellinus linteus on lipid composition and antioxidative system in rats fed high fat high cholesterol diet. Korean J. Food Sci. Nutr. 39, 71-77. https://doi.org/10.3746/jkfn.2010.39.1.071
  29. Sung, H. K., Choi, S. H. and Ahn, K. S. 1999. Study on the effects of curcumin on angiogenic inhibiton mechanim. Korean J. Oriental Med. Pathol. 13, 66-78.
  30. Vahouny, G. V., Toy, T., Gallo, L. L., Story, J., Kritechevsky, D., Cassidy, M., Grund, B. M. and Treadwell, C. R. 1978. Dietary fiber and lymphatic absorption of cholesterol in the rat. Am. J. Clin. Nutr. 34, 208-212.
  31. Yun, S. J., Yeon, J. Y., Kim, M. H., Kang, M. H., Kim, T. H., Son, Y. K. and Kim, M. H. 2009. The effects of Angelica keiskei Koidzumi and turmeric extract supplementation on the blood lippids and antioxidant and inflammatory markers in hypercholesterolemic adults in Korea. Korean J. Food Nutr. 4, 517-525.

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