The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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The isolation of Bacillus subtilis KYS-10 with antifungal activity against plant pathogens

식물 병원균에 대한 항진균 활성을 갖는 Bacillus subtilis KYS-10의 분리

  • Kang, Dae-Won (College of Life Science and Natural Resources, Wonkwang University) ;
  • Ryu, Il-Hwan (College of Life Science and Natural Resources, Wonkwang University) ;
  • Han, Seong-Soo (College of Life Science and Natural Resources, Wonkwang University)
  • 강대원 (원광대학교 생명자원과학대학) ;
  • 류일환 (원광대학교 생명자원과학대학) ;
  • 한성수 (원광대학교 생명자원과학대학)
  • Received : 2012.01.30
  • Accepted : 2012.05.19
  • Published : 2012.06.30
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Abstract

This study was investigated for the purpose of the isolation and identification of antagonistic bacteria with antifungal activity against plant pathogens. This bacteria denominated Bacillus subtilis KYS-10 and the optimum growth condition were 4% sucrose, 1% yeast extract, 0.2% $K_2HPO_4$, pH 7, 150 rpm, $30^{\circ}C$, 8 day. The antifungal activities against nine plant pathogens determined inhibition zone size by diffusion methods. The results, G. zeae (scab) 70 mm and P. grisea KACC 40439 (blast), P. capsici KACC 40177 (phytophthora blight) and C. destructans KACC 41077 (root rot of ginseng) 40~43 mm, and C. gloeosporioides KACC 43520 (ripe rot), C. gloesporioides KACC 40003 (anthracnose), S. shiraiana KACC 41065 (stem rot) and S. shiraiana (mulberry sclerotial disease) 35~39 mm and F. Oxysporum KACC 44452 (bulb rot of ginseng) 28 mm. From these experiment results, author suggest that Bacillus subtilis KYS-10 would be developed as a biological control agent thorough the field experimet in the near future.

본 연구는 9종 식물병원균에 대한 항진균 활성을 나타내는 길항세균을 분리하여 동정한 결과 Bacillus subtilis KYS-10로 명명하였고, sucrose 4%, yeast extract 1.0%, $K_2HPO_4$ 0.2%, pH 7의 최적배지에서 150 rpm, $30^{\circ}C$, 8일간 배양 시 가장 좋은 생육을 나타냈다. B. subtilis KYS-10의 9종 식물병원균에 대한 항진균 활성을 확산법에 의해 생육저지환의 크기를 측정한 결과 G. zeae (맥류 붉은곰팡이병) 70 mm로 가장 높았고, 35~39 mm의 생육저해환을 나타낸 식물병원균은 P. KACC 40439(벼도열병), P. capsici KACC 40177(고추역병균), C. destructans KACC 41077(인삼뿌리썩음병) 40~43 mm, C. gloeosporioides KACC 43520(포도 탄저병균), C. gloesporioides KACC 40003(고추 탄저병균), S. shiraiana KACC 41065(상추 균핵병균), S. shiraiana(오디 균핵병균) 있었으며, F. Oxysporum KACC 44452(인삼 부패병균)은 28 mm로 다종의 식물병원균에 대해 높은 저해활성을 보였다. 이상의 결과들은 추후 포장실험을 통하여 친환경 미생물제제로의 개발 가능성을 시사해 주고 있다.

Keywords

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