The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The Effect of Natural Mixture Supplementation on Histopathological and Histomorphometrical aspects in High Fat Diet-induced Obese Mice

천연 소재 복합물이 고지방사료로 유도된 비만 생쥐의 조직형태 및 조직병리학적 변화에 미치는 영향

  • Shin, In-Soon (Department of Herbal Pharmacology, R&D Team for NDOM (BK21)) ;
  • Choi, Hye-Min (Department of Herbal Pharmacology, R&D Team for NDOM (BK21)) ;
  • Ku, Sae-Kwang (Anatomy and Histology, R&D Team for NDOM (BK21), College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Mi-Ryeo (Department of Herbal Pharmacology, R&D Team for NDOM (BK21))
  • 신인순 (대구한의대학교 BK21 한방신약개발연구팀&한의과대학 본초약리학교실) ;
  • 최혜민 (대구한의대학교 BK21 한방신약개발연구팀&한의과대학 본초약리학교실) ;
  • 구세광 (대구한의대학교 BK21 한방신약개발연구팀&한의과대학 해부학교실) ;
  • 김미려 (대구한의대학교 BK21 한방신약개발연구팀&한의과대학 본초약리학교실)
  • Received : 2012.06.18
  • Accepted : 2012.06.29
  • Published : 2012.07.30
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Abstract

Objective : Obesity is often defined as a condition associated with accumulations of excessive body fats which resulting from disorder of energy balance in term of energy intake and energy expenditure. Methods : The effects of natural mixture (T) for inhibition of lipid metabolism on the liver, epididymal fat pads and pancreatic zymogen granules of high fat diet (HFD) supplied rats were observed by histopathology and histomorphometry. Results : As results of HFD supply, severe steatohepatitis such as increases of mean diameters of hepatocytes and the percentages regions of fatty changes was detected. In addition, hypertrophy of adipocytes (increase of mean diameters of epididymal fat pads) was also detected with dramatic decreases of pancreatic zymogen granules at histopathological and histomorphometrical observations. However, theses steatohepatitis and hypertrophy of adipocytes induced by HFD supply were inhibited by treatment of 5 % and 10 % T (T5, T10), respectively. Well corresponded as the results of adipocyte hypertrophy and steatohepatitis, the decreases of pancreatic zymogen granules were also dose-dependently inhibited by T treatment as compared with HFD control, respectively. Conclusion : In conclusion, based on the results, it is considered that test materials, T5 and T10, will be showed hepatoprotective and anti-obese effects, may be directly and/or indirectly mediated by pancreatic zymogen granules because they dose-dependently inhibited steatohepatitis, hypertrophy of adipocytes and decreases of pancreatic zymogen granules induced by HFD supply, respectively.

Keywords

References

  1. Hue GB. Pathology of obesity. Kor J Nutrition. 1990 ; 23 : 333-6.
  2. Sharrett AR, Ballantyne CM, Coady SA, Heiss G, Sorlie PD, Catellier D, Patsch W. Atherosclerosis risk in communities stud levels, triglyceride, lipoproein(a), apolipoprotein A-I and B, and HDL density subfractions: The atherosclerosis risk in communities (ARIC) Study. Circulation. 2001 ; 104 : 1108-13. https://doi.org/10.1161/hc3501.095214
  3. Joo DS, Lee JK, Kim OK, Cho SY, Lee DS, Je YK, Choi JW. Effect of Seatangle Oligosaccharide Drink on Oxidation of Serum Lipid and Bleeding and Plasma Clotting Time in Rats Fed a Hyperlipidemic Diet. J Kor Soc Food Sci Nutr. 2003 ; 32(8) : 1370-76. https://doi.org/10.3746/jkfn.2003.32.8.1370
  4. Lee HS, Choi MS, Lee YK, Park SH, Kim YJ. A Study on the Development of High-fiber Supplements for the Diabetic Patients - Effect of Seaweed Supplementation on the Lipid and Glucose Metabolism in Streptozotocin-induced Diabetic Rats. Kor J Soc Food Sci Nutr. 1996 ; 29(3) : 296-306.
  5. Record IR, Dreosit IE, Mclnerney JK. The antioxidant activity of genistein in vitro. J. Nutr. Biochemistry. 1995 ; 6 : 481-5. https://doi.org/10.1016/0955-2863(95)00076-C
  6. Wei H, Cai Q, Rahn R. Inhibition of UV light and fenton reaction-induced oxidative DNA damage by the soybean isoflacone genistein. Carcinogenesis. 1996 ; 17 : 73-7. https://doi.org/10.1093/carcin/17.1.73
  7. Sa JH, Shin IC, Jeong KJ, Shim TH, Oh HS, Kim YJ, Cheung EH, Kim GG, Choi OS. Antioxidative activity and chemical charcteristics from different organs of small black soybean (yak-kong) grown in the area of Jungsun. Kor J Food Sci Technol. 2003 ; 35 : : 309-15.
  8. Kaneda T, Hidaka Y, Kashiwai T, Tada H, Takano T, Nishiyama S, Amino N, Miyai K. Effect of coix seed on the changes in peripheral lymphocyte substs. Rinsho Byori. 1992 ; 40(2) : 179-181.
  9. Zhang Y, Hou G, Yue Y. The analgesic action of semen coicis on severe functional dysmenorrhea--a sequential trial observation. J Tradit Chin Med. 2000 ; 20(4) : 293-6.
  10. Ukita T, Tanimura A. Studies on the antitumor components in the seeds of coix lachrymajobi L. var. mayuen. Chemical & Pharmaceutical Bulletin. 1961 ; 9 : 43-6. https://doi.org/10.1248/cpb.9.43
  11. Otsuka H, Hirai Y, Nagao T, Yamasaki K. Anti-inflammatory activity of benzoxanoids from roots of Coixlachryma-jobi L. var. ma-yuen. J Nat Prod. 1988 ; 51 : 74-9. https://doi.org/10.1021/np50055a009
  12. Hu Q, Xu J, Chen S, Yang F. Antioxidant activity of black sesame seed (Sesamum indicum L.) by supercritical carbon dioxide extraction. J Agric Food Chem. 2004 ; 52 : 943-947. https://doi.org/10.1021/jf034485x
  13. Han BJ. A study of use of sesame and sesame oil in traditional korean cuisine. J East Asian Soc Dietary Life 15. 2005 ; 2 : 137-51.
  14. Hong JS, Kim YH, Lee KR, Kim MK, Cho YH, Park KH, Lee JB. Composition of organic acid and fatty acid in Pleutotus ostreatus, Lentinus edodes and Agaricus bisporus (in Korea). Kor J Food Sci Technol. 1988 ; 20 : 100-5.
  15. Pi-Sunyer FX. Health implications of obesity. Am J Clin Nutr. 1991 ; 53 : 1595S-1603S.
  16. Seidell JC, Verschuren WM, van Leer EM, Kromhout D. Overweight, underweight, and mortality. A prospective study of 48,287 men and women. Arch Intern Archives of Internal Medicine. 1996 ; 156 : 958-63. https://doi.org/10.1001/archinte.1996.00440090054006
  17. Lebovitz HE. Insulin resistance: definition and consequences. experimental and clinical endocrinology. Diabetes. 2001 ; 109 : 135-48.
  18. Goldstein BJ. Insulin resistance as the core defect in type 2 diabetes mellitus. Am J Cardiol. 2002 ; 90 : 3-10.
  19. Department of internal medicine seoul university colleage of medicine. Internal medicine. 1nd. ed. Seoul : Koonja publisher. 1996 : 852-62.
  20. Inzucchi SE. Oral antihyperglycemic therapy for type 2 diabetes: scientific review. J Ame Med Associ. 2002 ; 287 : 360-72. https://doi.org/10.1001/jama.287.3.360
  21. DiGirolamo M, Fine JB, Tagra K, Rossmanith R. Qualitative regional differences in adipose tissue growth and cellularity in male Wistar rats fed ad libitum. Am J Physiol. 1998 ; 274 : 1460-1467.
  22. Lindback SM, Gabbert C, Johnson BL, Smorodinsky E, Sirlin CB, Garcia N, Pardee PE, Kistler KD, Schwimmer JB. Pediatric nonalcoholic fatty liver disease: a comprehensive review. Adv Pediatr. 2010 ; 57 : 85-140. https://doi.org/10.1016/j.yapd.2010.08.006
  23. Cheong JY. Treatment of patients with non-alcoholic fatty liver disease. Kor J Hepatol. 2007 ; 13(2) : 9-16.
  24. Widhalm K, Ghods E. Nonalcoholic fatty liver disease: a challenge for pediatricians. Int J Obes (Lond). 2010 ; 34 : 1451-67. https://doi.org/10.1038/ijo.2010.185
  25. Miller NE. The evidence for the antiatherigenicity of high density lipoprotein in man. Lipids. 1978 ; 13 : 914-919. https://doi.org/10.1007/BF02533850
  26. Han JS, Han YB. The effect of high fat diet and dietary fiber on adipocyte of epididymal fat pads in rat. Kor J Nutri. 1994 ; 27 : 119-26.
  27. Aho HJ, Sternby B, Kallajoki M, Nevalainen TJ. Carboxyl ester lipase in human tissues and in acute pancreatitis. Int J Pancreatol. 1989 ; 5 : 123-134.
  28. Ichikawa A. Fine structural changes in response to hormonal stimulation of perfused canine pancreas. J Cell Biol. 1965 ; 24 : 369-385. https://doi.org/10.1083/jcb.24.3.369

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