Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Charactrization of Biological Activities of Rehmannia glutinosa Extracts

숙지황(Rehmannia glutinosa) 추출물의 생리활성

  • Cho, Young-Je (School of Food Science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University)
  • 조영제 (경북대학교 식품공학부/식품생물산업연구소)
  • Received : 2012.04.13
  • Accepted : 2012.07.14
  • Published : 2012.07.30
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Abstract

The content phenolic compounds in extracts from Rehmannia glutinosa was the highest in 40% ethanol extracts as $5.1{\pm}0.2mg/g$. DPPH scavenging activity of R. glutinosa extracts was high in water extracts and 40% ethanol extracts as 85~93%, ABTS radical cation decolorization of water extracts and 40% ethanol extracts was about the same as 55~62%, antioxidant protection factor (PF) was confirmed in water extracts and 40% ethanol extracts as 1.6~1.9 PF, and TBARs of water extracts and 40% ethanol extracts were concluded to have the similar antioxidant effects. The hypertension inhibitory activity of water extracts and 40% ethanol extracts from R. glutinosa indicated the activities as 87.2% and 81.1%, anti-gout activity was determined very low in R. glutinosa extracts and antimicrobial activity against skin microorgasm was confirmed, and tyrosinase inhibitory activity was determined as 70.2% in 40% ethanol extracts, it was expected the whitening effects in 40% ethanol extracts. The elastase inhibitory activity which are related to the wrinkle cause was observed in water extracts and 40% ethanol extracts as 76.2% and 57.2%. The hyaluronidase inhibitory activity to R. glutinosa extracts was observed weakly in only 40% ethanol extracts of $200{\mu}g/ml$ phenolic content as 5.1%.

숙지황(Rehmannia glutinosa)으로부터 유용성분 추출은 40% 에탄올 추출물에서 $5.1{\pm}0.2mg/g$으로 가장 높은 phenolic compound 함량을 나타내었다. 숙지황 추출물의 항산화활성은 전자공여능의 경우 물 추출물과 40% ethanol 추출물 모두에서 85~93%의 높은 전자공여능이 확인되었으며, ABTS radical cation decolorization의 경우 물 추출물과 40% ethanol 추출물이 55~62%의 비슷한 수준의 항산화효과를 효과를 나타내었고, antioxidant protection factor (PF)는 $200{\mu}g/ml$의 농도에서 물 추출물과 40% ethanol 추출물 모두에서 1.6~1.9 PF의 높은 항산화력이 확인되었고, TBARs는 물 추출물과 40% ethanol 추출물이 비슷한 수준의 항산화효과를 효과를 나타내는 것으로 측정되었다. 숙지황 추출물의 고혈압억제효과는 물 추출물에서 87.2%, 40% ethanol 추출물에서 81.1%의 저해활성을 나타내었다. 관절염억제효과는 매우 낮게 측정 되었으며, 피부상재균에 대한 항균효과는 확인되었다. 숙지황 추출물의 tyrosinase 저해 활성은 40% ethanol 추출물에서 70%의 높은 저해효과가 확인되어 미백효과를 기대할 수 있었다. 주름 생성과 관련된 elastase 저해 효과는 물 추출물에서 76%, ethanol 추출물 57%의 높은 elastase 억제효과를 관찰할 수 있었다. 숙지황 추출물에 대한 hyaluronidase 저해활성은 40% ethanol 추출물에서만 $200{\mu}g/ml$ phenolic의 농도에서 약 5%의 매우 낮은 hyaluronidase 저해활성을 나타내었다.

Keywords

References

  1. Andarwulan, N. and Shetty, K. 1999. Phenolic content in differentiated tissue cultures of untransformed and Agrobacterium-transformed roots of anise(Pimpinella anisum L.). J. Agric. Food Chem. 47, 1776-1780. https://doi.org/10.1021/jf981214r
  2. Blios, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1200.
  3. Boo, H. O., Hwang, S. J., Bae, C. S., Park, S. H. and Song, W. S. 2011. Antioxidant activity according to each kind of natural plant pigment. Kor. J. Plant Res. 24, 105-112. https://doi.org/10.7732/kjpr.2011.24.1.105
  4. Buege, J. A. and Aust, S. D. 1978. Microsomal lipid peroxidation. Method Enzymol. 105, 302-310.
  5. Chen, L. Z., Feng, X. W. and Zhou, J. H. 1995. Effects of Rehmannia glutinosa polysaccharide b on T-lymphocytes in mice bearing sarcoma 180. Chung. Kuo. Yao. Li. Hsueh. Pao. 16, 337-340.
  6. Cho, S. I. 2003. Antioxidative effects of Rehmanniae Radix Preparata on toxic agent induced kidnet cell injury. Kor. J. Herbology 18, 119-126.
  7. Choi, S. I., Lee, Y. M. and Heo, T. R. 2003. Screening of hyaluronidase inhibitory and free radical scavenging activity in vitro of traditional herbal medicine extracts. Kor. J. Biotech. Bioeng. 18, 282-288.
  8. Chung, H. J. 1989. Studies on variation of constituents and enzyme activities of Rehmannia Radix by processing. Department of Pahamacy, Graduate school of Sookmyung Woman's University, Korea. pp. 1-12.
  9. Conner, D. E. and Beuchat, L. R. 1984. Sensitivity of heat-stressed yeasts to essential oils of plants. Appl. Environ. Microbial. 47, 229-233.
  10. Cushman, D. W., Cheung, H. S., Sabo, E. F. and Ondetti, M. A. 1977. Design of potent competitive inhibitors of angiotensin-converting enzyme. Carboxylalkanoyl and mercaptoalka noyl amino acids. Biochemistry 16, 5484-5491. https://doi.org/10.1021/bi00644a014
  11. Duval, B. and Shetty, K. 2001. The stimulation of phenolics and antioxidant activity in pea (Pisum sativum) elicited by genetically transformed anise root extract. J. Food Biochem. 25, 361-377. https://doi.org/10.1111/j.1745-4514.2001.tb00746.x
  12. Folin, O. and Denis, W. 1912. On phosphotungastic-phosphomolybetic compounds as color reagents. J. Biol. Chem. 12, 239-249.
  13. Ghosh, P. 1994. The role of hyaluronic acid (hyaluronan) in health and disease: interactions with cells, cartilage and components of synovial fluid. Clin. Exp. Rheumatol. 12, 75-82.
  14. Han, K. H., Lee, J. C., Kim, J. H. and Lee, J. S. 2002. Manufacture and physiological functionality of Korean traditional liquor by using purple-fleshed sweet potato. Kor. J. Food Sci. Technol. 34, 673-677.
  15. Jang, M. S., Yang, W. M., Yu, T. W., Kim, D. R., Park, E. H., Ko, E. B., Choi, M. J., Kim, H. Y., Oh, J. H., Shin, K. J., Yoon, J. W. and Park, S. K. 2007. Antioxidant effects of Rehmanniae Radix Preparat in GC-1 cell. Kor. J. Herbology 22, 81-86.
  16. Jeong, H. J. and Kim, I. H. 1990. Comparative studies om the antidiabetic activities of Rehmannia Radices. J. Pharm. Sci. 4, 22-31.
  17. Ju, I. S. and Cho, Y. J. 2009. Purification and identification of phenol compounds with inhibitory activity on Helicobacter pylori from Rhododendron mucronulatum Flos. extracts. J. Life Sci. 19, 1125-1131. https://doi.org/10.5352/JLS.2009.19.8.1125
  18. Kim, E. Y., Baik, I. H., Kim, J. H., Kim, S. R. and Rhyu, M. R. 2004. Screening of the antioxidant activity of some medicinal plants. Kor. J. Food Sci. Technol. 36, 333-338.
  19. Kim, J. H. 2006. Biological activity of phenolic compound from Herb and Oriental medicinal resource. Kyungpook national university MS thesis, Korea, p. 17-22.
  20. Kim, S. J., Lim, J. H., Lee, R. S. and Lee, J. S. 1996. Extraction and a characteristics of a purple sweet potato pigment. Kor. J. Food Sci. 28, 345-347.
  21. Kwak, Y. J., Lee, D. H., Kim, N. M. and Lee, J. S. 2005. Screening and extraction condition of anti-skin aging elastase inhibitor from medicinal plants. Kor. J. Med. Crop. Sci. 13, 213-216.
  22. Lee, N. H., Yang, H. C., Bu, H. J., Jung, D. S., Lee, S. J. and Riu, K. Z. 2001. Screening of the tyrosinase inhibition and hyaluronidase inhibition activities and radical scavenging effects using plants in Cheju. Kor. J. Pharmaco. 32, 175-180.
  23. Lee, H. 2011. Effects of Lxeris dentata extracts on lowering lipid and antioxidation. Kor. J. Plant Res. 24, 55-60. https://doi.org/10.7732/kjpr.2011.24.1.055
  24. Moon, S. H. and Lee, Y. J. 2001. Studies on the digestive function of Rehmanniae Radix produced by different processes. Kor. J. Herbology 16, 65-77.
  25. Park, J. B. 1993. Effects of Rehmannia Radix aqua-acupuncture at the meridian point BL 23 on the renal function in two-kidney one goldblatt hypertensive rats. Department of oriental medicine, graduate school of Wonkang University, Korea, pp. 2-9.
  26. Pellegrin, N., Roberta, R., Min, Y. and Catherine, R. E. 1998. Screening of diatry carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid)radical cation decolorization assay. Method Enzymol. 299, 379-389.
  27. Soffer, R. L. 1976. Angiotensin-converting enzyme and the rela gulation of vasoactive peptides. Ann. Rev. Biochem. 45, 73-78. https://doi.org/10.1146/annurev.bi.45.070176.000445
  28. Storch, J. and Feber, E. 1988. Detergent-amplified chemilum-inescence of lucigenin for determination of superoxide anion production by NADPH oxidase and xanthine oxidase. Anal. Biochem. 169, 262-267. https://doi.org/10.1016/0003-2697(88)90283-7
  29. Stirpe, F. and Crote, E. D. 1969. The regulation of rat liver xanthine oxidase. J. Biol. Chem. 244, 3855-3858.
  30. Tomoda, M., Miyamoto, H. and Shimizu, N. 1994. Structural features and anti-complementary activity of rehmannan SA, a polysaccharide from the root of Rehmannia glutinosa. Chem. Pharm. Bull. 42, 1666-1668. https://doi.org/10.1248/cpb.42.1666
  31. Tomoda, M., Miyamoto, H., Shimizu, N., Gonoda, R. and Ohara, N. 1994. Characterization of two polysaccharides having activity on the reticuloendothelial system from the root of Rehmannia glutinosa. Chem. Pharm. Bull. 42, 625-629. https://doi.org/10.1248/cpb.42.625
  32. Tomoda, M., Miyamoto, M., Shimizu, N., Gonoda, R. and Ohara, N. 1994. Two acidic polysaccharides having reticuloendothelial system-potentiating activity from the raw root of Rehmannia glutinosa. Biol. Pharm. Bull. 17, 1456-1459. https://doi.org/10.1248/bpb.17.1456
  33. Tsuji, N., Moriwaki, S., Suzuki, Y., Takema, Y. and Imokawa, G. 2001. The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Phytochem. Photobiol. 74, 283-290. https://doi.org/10.1562/0031-8655(2001)074<0283:TROESB>2.0.CO;2
  34. Xu, M. L., Wang, L. and Wang, M. H. 2011. The antioxidant and anticancer effects of MeOH extract of Liriodendron tulipifera. Kor. J. Plant Res. 24, 23-29. https://doi.org/10.7732/kjpr.2011.24.1.023
  35. Yagi, A., Kanbara, T. and Morinobu, N. 1986. The effect of tyrosinase inhibition for aloe. Planta. Medica. 3981, 517-519.
  36. Yang, Y. J., Kim, H. J., Kang, S. H. and Kang, S. C. 2011. Screening of natural herb resources for antioxidative effects in Korea. Kor. J. Plant Res. 24, 1-9. https://doi.org/10.7732/kjpr.2011.24.1.001
  37. Zielinski, H. and Kozlowska, H. 2000. Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions. J. Agric. Food Chem. 48, 2008-2016. https://doi.org/10.1021/jf990619o

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