Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Antifungal activity of essential oil from Cryptomeria japonica against dermatophytic fungi

삼나무 정유의 표재성 진균에 대한 항진균 활성

  • Kim, Seon-Hong (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Lee, Su-Yeon (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Hong, Chang-Young (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Jeong, Han-Seob (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Park, Mi-Jin (Korea Forest Research Institute) ;
  • Choi, In-Gyu (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
  • 김선홍 (서울대학교 농업생명과학대학 산림과학부) ;
  • 이수연 (서울대학교 농업생명과학대학 산림과학부) ;
  • 홍창영 (서울대학교 농업생명과학대학 산림과학부) ;
  • 정한섭 (서울대학교 농업생명과학대학 산림과학부) ;
  • 박미진 (국립산림과학원 녹색자원이용부 바이오에너지연구과) ;
  • 최인규 (서울대학교 농업생명과학대학 산림과학부)
  • Received : 2012.02.03
  • Accepted : 2012.07.09
  • Published : 2012.07.25
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Abstract

In this study, the antifungal activity of Cryptomeria japonica essential oil against superficial and allergic fungi, Trichophyton schoenleinii, Trichophyton tonsurans, Trichophyton mentagrophytes, Trichophyton rubrum, Epidermophyton floccosum, and Aspergillus fumigatus, was evaluated for determining the potential compound as dermatitis treatment. Minimum Inhibitory Concentration (MIC) measurement, TLC bioassay and agar dilution methods were used for determining the antifungal activity of crude essential oil and its fractions from C. japonica. Also, their major constituents were analyzed by GC/MS. The MICs were below 500 ppm at all superficial fungi, and spot 1 of C. japonica essential oil showed highly effective antifungal activity by TLC bioassay. In antifungal activity by agar dilution methods, crude oil showed high antifungal effect at more than 500 ppm and fraction D was significantly effective at even 100 ppm except for A. fumigatus. The major compounds of spot 1 and fraction D of C. japonica oil determined by GC/MS were elemol, ${\gamma}$-eudesmol, and ${\beta}$-eudesmol, which could be used as atopic dermatitis treatment material.

본 연구에서는 표재성 진균에 의한 피부질환 개선용 소재로의 개발을 위해 삼나무 정유의 표재성 알레르기성 진균, Trichophyton schoenleinii, Trichophyton tonsurans, Trichophyton mentagrophytes (interdigitale), Trichophyton rubrum, Epidermophyton floccosum, Aspergillus fumigatus에 대한 항진균 활성을 평가하여 삼나무 정유의 표재성 진균에 의한 피부질환 개선 소재로의 개발 가능성을 평가하고자 하였다. 항진균 활성 평가는 삼나무 정유의 MIC (Minimum Inhibitory Concentration) 측정과 TLC bioassay, 한천희석법을 통해 측정하였다. 또한 활성 성분의 주성분을 확인하기 위해 GC/MS 분석을 실시하였다. 삼나무 정유 crude oil의 MIC는 모든 균에서 500 ppm 이하의 농도 값을 나타냈으며, TLC bioassay를 통해 spot 1에서 항진균 효과를 확인하였다. 한천희석법을 통한 항진균 활성은 A. fumigatus를 제외한 모든 진균에 대해 삼나무 정유 crude oil의 경우, 500 ppm 이상에서 100%의 활성을 나타냈고, 분획 D의 경우, 100 ppm에서도 100%의 활성을 나타냈다. TLC bioassay를 통해 항진균 활성을 나타낸 spot 1과 한천희석법을 통해 활성을 나타낸 분획 D를 GC/MS로 분석한 결과 주성분은 elemol, ${\gamma}$-eudesmol, ${\beta}$-eudesmol이었다. 따라서 elemol, eudesmol 등은 표재성 진균에 의한 피부질환의 개선 소재로의 이용 가능성이 충분하다고 사료된다.

Keywords

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