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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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The inhibitory effects of Nardostachys jatamansi on alcoholic chronic pancreatitis

  • Bae, Gi-Sang (Hanbang Body-fluid Research Center, College of Oriental Medicine, Wonkwang University) ;
  • Park, Kyoung-Chel (Department Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Koo, Bon-Soon (Department Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Choi, Sun-Bok (Department Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Jo, Il-Joo (Department Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Choi, Chang-Min (Department Obstetrics and Gynecology, College of Oriental Medicine, Wonkwang University) ;
  • Song, Ho-Joon (Department Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Park, Sung-Joo (Hanbang Body-fluid Research Center, College of Oriental Medicine, Wonkwang University)
  • Received : 2012.04.05
  • Accepted : 2012.04.24
  • Published : 2012.07.31
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Abstract

Nardostachys jatamansi (NJ) belonging to the Valerianaceae family has been used as a remedy for gastrointestinal inflammatory diseases for decades. However, the potential for NJ to ameliorate alcoholic chronic pancreatitis (ACP) is unknown. The aim of this study was to examine the inhibitory effects of NJ on ACP. C57black/6 mice received ethanol injections intraperitoneally for 3 weeks against a background of cerulein-induced acute pancreatitis. During ACP, NJ was ad libitum administrated orally with water. After 3 weeks of treatment, the pancreas was harvested for histological examination. NJ treatment increased the pancreatic acinar cell survival (confirmed by amylase level testing) and reduced collagen deposition and pancreatic stellate cell (PSC) activation. In addition, NJ treatment reduced the activation but not death of PSC. In conclusion, our results suggest that NJ attenuated ACP through the inhibition of PSC activation.

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References

  1. Apte, M. V., Pirola, R. C. and Wilson, J. S. (2011) The fibrosis of chronic pancreatitis: new insights into the role of pancreatic stellate cells. Antioxid. Redox Signal. 15, 2711-2722. https://doi.org/10.1089/ars.2011.4079
  2. Apte, M. V., Pirola, R. C. and Wilson, J. S. (2010) Mechanisms of alcoholic pancreatitis. J. Gastroenterol. Hepatol. 25,1816-1826. https://doi.org/10.1111/j.1440-1746.2010.06445.x
  3. Lankisch, P. G., Lohr-Happe, A., Otto, J. and Creutzfeldt, W. (1993) Natural course in chronic pancreatitis. Pain, exocrine and endocrine pancreatic insufficiency and prognosis of the disease. Digestion 54, 148-155. https://doi.org/10.1159/000201029
  4. Kloppel, G., Detlefsen, S. and Feyerabend, B. (2004) Fibrosis of the pancreas: the initial tissue damage and the resulting pattern. Virchows Arch. 445, 1-8.
  5. Apte, M. V., Haber, P. S., Applegate, T. L., Norton, I. D., McCaughan, G. W., Korsten, M. A., Pirola, R. C. and Wilson, J. S. (1998) Periacinar stellate shaped cells in rat pancreas: identification, isolation, and culture. Gut 43, 128-133. https://doi.org/10.1136/gut.43.1.128
  6. Haber, P. S., Keogh, G. W., Apte, M. V., Moran, C. S., Stewart, N. L., Crawford, D. H., Pirola, R. C., McCaughan, G. W., Ramm, G. A. and Wilson, J. S. (1999) Activation of pancreatic stellate cells in human and experimental pancreatic fibrosis. Am. J. Pathol. 155, 1087-1095. https://doi.org/10.1016/S0002-9440(10)65211-X
  7. Bae, G. S., Park, H. J., Kim, D. Y., Song, J. M., Kim, T. H., Oh, H. J., Yun, K. J., Park, R. K., Lee, J. H., Shin, B. C., Sim, H. J., Hong, S. P., Song, H. J. and Park, S. J. (2010) Nardostachys jatamansi protects against cerulein-induced acute pancreatitis. Pancreas 39, 520-529. https://doi.org/10.1097/MPA.0b013e3181bd93ce
  8. Bae, G. S., Seo, S. W., Kim, M. S., Park, K. C., Koo, B. S., Jung, W. S., Cho, G. H., Oh, H. C., Yun, S. W., Kim, J. J., Kim, S. G., Hwang, S. Y., Song, H. J. and Park, S. J. (2011) The roots of Nardostachys jatamansi inhibits lipopolysaccharide- induced endotoxin shock. J. Nat. Med. 65, 63-72. https://doi.org/10.1007/s11418-010-0458-x
  9. Uniyal. M. R. and Issar, R. K. (1969) Commercially and traditionally important medicinal plants of Mandakini Valley of Uttrakhand. Himalayas. J. Res. Indian Med. 4, 83.
  10. Chatterji, A. and Prakashi, S. C. (1997) The Treatise on Indian Medicinal Plants. Vol 5. pp. 99-100, National Institute of Science Communication, New Delhi, India.
  11. Arora, R. B. (1965) Nardostachys jatamansi VA Chemical, Pharmacological and Clinical Appraisal. Monograph Special Series. Vol 51. Indian Council of Medical Research, New Delhi, India.
  12. Masamune, A., Watanabe, T., Kikuta, K. and Shimosegawa, T. (2009) Roles of pancreatic stellate cells in pancreatic inflammation and fibrosis. Clin. Gastroenterol. Hepatol. 7, S48-54. https://doi.org/10.1016/j.cgh.2009.07.038
  13. Omary, M. B., Coulombe, P. A., and McLean, W. H. (2004) Intermediate filament proteins and their associated diseases. N. Engl. J. Med. 351, 2087-2100. https://doi.org/10.1056/NEJMra040319
  14. Charrier, A. L. and Brigstock, D. R. (2010) Connective tissue growth factor production by activated pancreatic stellate cells in mouse alcoholic chronic pancreatitis. Lab. Invest. 90, 1179-1188. https://doi.org/10.1038/labinvest.2010.82
  15. Ohashi, S., Nishio, A., Nakamura, H., Kido, M., Ueno, S., Uza, N., Inoue, S., Kitamura, H., Kiriya, K., Asada, M., Tamaki, H., Matsuura, M., Kawasaki, K., Fukui, T., Watanabe, N., Nakase, H., Yodoi, J., Okazaki, K. and Chiba, T. (2006) Protective roles of redox-active protein thioredoxin-1 for severe acute pancreatitis. Am. J. Physiol. Gastrointest. Liver Physiol. 290, G772-781. https://doi.org/10.1152/ajpgi.00425.2005
  16. Oruc, N. and Whitcomb, D. C. (2004) Theories, mechanisms, and models of alcoholic chronic pancreatitis. Gastroenterol. Clin. North Am. 33, 733-750. https://doi.org/10.1016/j.gtc.2004.07.014
  17. Apte, M. V. and Wilson, J. S. (2003) Alcohol-induced pancreatic injury. Best Pract. Res. Clin. Gastroenterol. 17, 593-612. https://doi.org/10.1016/S1521-6918(03)00050-7
  18. Schenker, S. and Montalvo, R. (1998) Alcohol and the pancreas. Recent Dev. Alcohol 14, 41-65. https://doi.org/10.1007/0-306-47148-5_3
  19. Whitcomb, D. C., Ulrich, C. D. (1999) Hereditary pancreatitis: new insights, new directions. Baillieres Best Pract. Res. Clin. Gastroenterol. 13, 253-263. https://doi.org/10.1053/bega.1999.0023
  20. Omary, M. B., Lugea, A., Lowe, A. W. and Pandol, S. J. (2007) The pancreatic stellate cell: a star on the rise in pancreatic diseases. J. Clin. Invest. 117, 50-59. https://doi.org/10.1172/JCI30082
  21. Apte, M. V., Haber, P. S., Darby, S. J., Rodger, S. C., McCaughan, G. W, Korsten, M. A., Pirola, R. C. and Wilson, J. S. (1999) Pancreatic stellate cells are activated by proinflammatory cytokines: implications for pancreatic fibrogenesis. Gut 44, 534-541. https://doi.org/10.1136/gut.44.4.534
  22. Apte, M. V., Pirola, R. C. and Wilson, J. S. (2006) Battle-scarred pancreas: role of alcohol and pancreatic stellate cells in pancreatic fibrosis. J. Gastroenterol. Hepatol. 21, S97-S101. https://doi.org/10.1111/j.1440-1746.2006.04587.x
  23. Schneider, E., Schmid-Kotsas, A., Zhao, J., Weidenbach, H., Schmid, R. M., Menke, A., Adler, G., Waltenberger, J., Grünert, A. and Bachem, M. G. (2001) Identification of mediators stimulating proliferation and matrix synthesis of rat pancreatic stellate cells. Am. J. Physiol. Cell Physiol. 281, C532-543. https://doi.org/10.1152/ajpcell.2001.281.2.C532
  24. Gullo, L., Barbara, L. and Labo, G. (1988) Effect of cessation of alcohol use on the course of pancreatic dysfunction in alcoholic pancreatitis. Gastroenterology 95, 1063-1068. https://doi.org/10.1016/0016-5085(88)90184-9
  25. Lugea, A., Nan, L., French, S. W., Bezerra, J. A., Gukovskaya, A. S. and Pandol, S. J. (2006) Pancreas recovery following cerulein-induced pancreatitis is impaired in plasminogen-deficient mice. Gastroenterology 131, 885-899. https://doi.org/10.1053/j.gastro.2006.06.023
  26. Vonlaufen, A., Phillips, P. A., Xu, Z., Zhang, X., Yang, L., Pirola, R. C., Wilson, J. S. and Apte, M. V. (2010) Withdrawal of alcohol promotes regression while continued alcohol intake promotes persistence of LPS-induced pancreatic injury in alcohol-fed rats. Gut 60, 238-246.
  27. Schwer, C. I., Guerrero, A. M., Humar, M., Roesslein, M., Goebel, U., Stoll, P., Geiger, K. K., Pannen, B. H., Hoetzel, A. and Schmidt, R. (2008) Heme oxygenase-1 inhibits the proliferation of pancreatic stellate cells by repression of the extracellular signal-regulated kinase1/2 pathway. J. Pharmacol. Exp. Ther. 327, 863-871. https://doi.org/10.1124/jpet.108.136549
  28. Li, L., Bimmler, D., Graf, R., Zhou, S., Sun, Z., Chen, J., Siech, M. and Bachem, M. G. (2011) PSP/reg inhibits cultured pancreatic stellate cell and regulates MMP/TIMP ratio. Eur. J. Clin. Invest. 41, 151-158. https://doi.org/10.1111/j.1365-2362.2010.02390.x
  29. Madro, A., Korolczuk, A., Czechowska, G., Celiński, K., Słomka, M., Prozorow-Król, B. and Korobowicz, E. (2008) RAS inhibitors decrease apoptosis of acinar cells and increase elimination of pancreatic stellate cells after in the course of experimental chronic pancreatitis induced by dibutyltin dichloride. J. Physiol. Pharmacol. 59, 239-249.
  30. Rickmann, M., Vaquero, E. C., Malagelada, J. R. and Molero, X. (2007) Tocotrienols induce apoptosis and autophagy in rat pancreatic stellate cells through the mitochondrial death pathway. Gastroenterology 132, 2518-2132. https://doi.org/10.1053/j.gastro.2007.03.107
  31. Long, D., Lu, J., Luo, L., Guo, Y., Li, C., Wu, W., Shan, J., Li, L., Li, S., Li, Y., Lin, T. and Feng, L. (2011) Effects of octreotide on activated pancreatic stellate cell-Induced pancreas graft Fibrosis in rats. J. Surg. Res. 176, 248-259.
  32. Aoki, H., Ohnishi, H., Hama, K., Shinozaki, S., Kita, H., Osawa, H., Yamamoto, H., Sato, K., Tamada, K., and Sugano, K. (2007) Cyclooxygenase-2 is required for activated pancreatic stellate cells to respond to proinflammatory cytokines. Am. J. Physiol. Cell Physiol. 292, C259-268. https://doi.org/10.1152/ajpcell.00030.2006
  33. Shinji, T., Ujike, K., Ochi, K., Kusano, N., Kikui, T., Matsumura, N., Emori, Y., Seno, T. and Koide, N. (2002) Establishment of a novel collagenase perfusion method to isolate rat pancreatic stellate cells and investigation of their gene expression of TGFbeta1, type I collagen, and CTGF in primary culture or freshly isolated cells. Acta. Med. Okayama 56, 211-218.

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