한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제16권2호
  • /
  • Pages.77-85
  • /
  • 2012
  • /
  • 2465-9525(pISSN)
  • /
  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

담수순화 감성돔(Acanthopagrus schlegeli) 정자의 냉동보존 조건별 활성평가

Assessment of Sperm Activity of Black Porgy(Acanthopagrus schlegeli) Acclimated in Freshwater on Cryopreservation Condition

  • 정민환 (국립수산과학원 양식관리과) ;
  • 임한규 (국립수산과학원 양식관리과) ;
  • 도용현 (국립수산과학원 양식관리과) ;
  • 김종현 (국립수산과학원 양식관리과) ;
  • 손맹현 (국립수산과학원 양식관리과) ;
  • 장영진 (부경대학교 해양바이오신소재학과)
  • Jeong, Min-Hwan (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Lim, Han-Kyu (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Do, Yong-Hyun (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Kim, Jong-Hyun (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Son, Maeng-Hyun (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Chang, Young-Jin (Dept. of Marine Bio-materials and Aquaculture, Pukyong National University)
  • 투고 : 2012.01.18
  • 심사 : 2012.05.21
  • 발행 : 2012.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

담수에서 사육한 감성돔 Acanthopagrus schlegeli(담수감성돔) 냉동/해동 정자의 생존율이 가장 높았던 결빙억제제(cryprotective agent, CPA)는 dimethyl sulfoxide(DMSO)였으며, 다음으로 glycerol, ethylene glycol(EG) 그리고 methanol 순이었다. 해동 후 정자운동성은 glycerol, DMSO, EG, methanol 순으로 높았으며, 이상의 결과들은 해수에서 사육한 감성돔(해수감성돔)에서도 나타났다. 담수 및 해수감성돔 정자의 냉동보존시 적정 CPA는 DMSO와 glycerol이었으며, 생존율과 정자운동성이 가장 높았던 적정 농도는 모두 10%였다. 특히 10% DMSO로 냉동보존한 담수 및 해수감성돔 해동정자의 생존율과 정자운동성은 10% glycerol로 냉동보존한 해동정자보다 높게 나타났다. 냉동보존기간에 따른 담수 및 해수감성돔 해동정자의 생존율과 정자운동성을 측정한 결과, 대부분 보존기간이 길어질수록 감소하였으며, CPA의 농도가 높을수록 생존율과 정자운동성은 급격히 감소하였다. 그러나 5% DMSO로 냉동보존한 해동정자의 생존율 및 정자운동성은 차이는 보이지 않았다.

Various cryoprotective agents (CPA) were tested to establish the best conditions for the cryopreservation of sperm from black porgy Acanthopagrus schlegeli acclimated and raised in freshwater (BFW). Survival rates of frozen/thawed sperm from BFW were higher in the order of dimethy sulfoxide (DMSO), glycerol, ethylene glycol (EG) and methanol. Sperm motility was higher in the order of glycerol, DMSO, EG and methanol. These effects were the same in thawed sperm from black porgy raised in seawater (BSW). Thus, optimum CPA for sperm cryopreservation of BFW and BSW were DMSO and glycerol where the highest survival rates and sperm motility were found at the concentration of 10%. In particular, the survival rates and motility of thawed sperm from BFW and BSW after cryopreservation using 10% DMSO were better than when cryopreserved using 10% glycerol. On the other hand, for the thawed sperm from both BFW and BSW, the longer the preservation period was, the lower the survival rates and sperm motility were. Notably, the higher the concentration of CPA was, the lower the survival rates and sperm motility were.

키워드

참고문헌

  1. Billard R, Cosson J, Noveiri SB, Pourkazemi M (2004) Cryopreservation and short-term storage of sturgeon sperm, a review. Aquaculture 236:1-9. https://doi.org/10.1016/j.aquaculture.2003.10.029
  2. Blaxter JHS (1953) Sperm storage and cross fertilization of spring and autumn spawning herring. Nature 172: 1189-1190.
  3. Blom E (1950) A one-minute live-dead sperm stain by means of eosin-nigrosin. Fertil Steril 1:176-177. https://doi.org/10.1016/S0015-0282(16)30125-X
  4. Chang YJ, Chang YJ (2002) Milt properties of four flatfish species and fine structure of their cryopreserved spermatozoa. J Fish Sci Tech 5:87-96.
  5. Chang YJ, Chang YJ, Lim HK (1997) Cryopreservation of tiger puffer (Takifugu rubripes) sperm. Dev Reprod 1:29-36.
  6. Chang YJ, Lim HK, Chang YJ, Kim HS (1999a) Sperm cryopreservation and fertility of post-thaw sperm on river puffer, Takifugu obscurus. J Aquacult 12:1-5.
  7. Chang YJ, Lim HK, Chang YJ, Kim HS, Huh HT (1999b) Physico-chemical properties and cold storage of river puffer (Takifugu obscurus) milt. J Kor Fish Soc 32: 243-246.
  8. Chao NH, Chao WC, Liu KC, Liao IC (1986) The biological properties of black porgy, Acanthopagrus schlegeli and its cryopreservation. Proceedings of the National Science Council of the Republic of China, Part B life Sciences 10:145-149.
  9. Chen YK, Liu QH, Li J, Xiao ZZ, Xu SH, Shi XH, Ma D (2010) Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm. Cryobiology 61:189-193. https://doi.org/10.1016/j.cryobiol.2010.07.003
  10. Choi HY, Jo PG, Kim TI, Bai SC, Chang YJ (2007) The effects of cryopreservation on fine structures of pearl oyster (Pinctada fucata martensii) larvae. Dev Reprod 11:79-84.
  11. Chung JK, Lim HK, Son MH, Kim JH, Jeong MH, Chang YJ (2011) Effects of concentration and immersion time of three cryoprotective agents on the embryos development of Abalone Haliotis discus hannai. Dev Reprod 15:301-308.
  12. Ciereszko A, Dabrowski K (1993) Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using a spectrophotometric technique. Aquaculture 109:367-373. https://doi.org/10.1016/0044-8486(93)90175-X
  13. Fribourgh JH (1966) The application of a differential ataing method to low-temperature studieds on goldfish spermatozoa. Prog Fish-Cult 28:227-231. https://doi.org/10.1577/1548-8640(1966)28[227:TAOADS]2.0.CO;2
  14. Gwo JC, Kurokura H, Hirano R (1993) Cryopreservation of spermatozoa from rainbow trout, common carp and marine puffer. Nippon Suisan Gakkaishi 59:777-782. https://doi.org/10.2331/suisan.59.777
  15. Jamieson BGM (1991) Fish Evolution and Systematics: Evidence from Spermatozoa. With a survey of lophophorate, echinodern and photochordate sperm and an account of gamete cryopreservation. Cambridge University Press, Cambridge. xiv +319 pp.
  16. Jeong MH, Lim HK, Kim YS, Kim SY, Chang YJ (2010) Sperm collection time, sex steroid hormones and gonadal development of black porgy, Acanthopagrus schlegeli acclimated in freshwater. Kor J Fish Aquat Sci 43:474-481.
  17. Kho KH (2007) Effects of cryoprotectants and diluents on cryopreservation of the red seabream, Pagrus major sperm. Kor J Ichthyol 19:173-177.
  18. Lahnsteiner F, Mansour N, Weismann T (2002) A new technique for insemination of large egg batches with cryopresered semen in the rainbow trout. Aquaculture 209:359-367. https://doi.org/10.1016/S0044-8486(01)00869-9
  19. Lahnsteiner F, Weismann T, Patzner RA (1997) Methanol as cryoprotectant and the suitability of 1.2 mL and 5 mL straws for cryopreservation of semen from salmonid fishes. Aquacult Res 28:471-479. https://doi.org/10.1111/j.1365-2109.1997.tb01065.x
  20. Lim HK, Chang YJ (1998) Effects of diluents and cryoprotectants on cryopreservation of black porgy (Acanthopagrus schlegeli) sperm. J Aquacult 11:67-75.
  21. Lim HK, An CM, Noh GA, Min BH (2007) Effects of diluents and cryoprotectants on sperm cryopreservation in starry flounder (Platichthys stellatus). J Aquacult 20:173-177.
  22. Lim HK, Lee CH, Min BH, Lee JU, Lee CS, Seong KB, Lee SM (2008) Effects of diluents and cryopretectants on sperm cryoperservation of masou salmon, Oncorhynchus masou masou. J Kor Fish Soc 41:267-271.
  23. Liu QH, Chen YK, Xiao ZZ, Li J, Xu SH, Shi XH (2010) Effect of storage time and cryoprotectant concentretions on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck and Schlegel, 1983). Aquacult Res 41:89-95.
  24. Min BH, Kim BK, Hur JW, Bang IC, Byun SK, Choi CY, Chang YJ (2003) Physiological responses during freshwater acclimation of seawater-cultured black porgy (Acanthopagrus schlegeli). Kor J Ichthyol 15:265-275.
  25. Strüssmann CA, Renard P, Ling H, Takashima F (1994) Motility of pejerrey Odontesthes bonariensis spermatozoa. Fish Sci 60:9-13. https://doi.org/10.2331/fishsci.60.9