Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Antimicrobial Activity of Ginger(Zingiber officinale Roscoe) Oleoresin by Supercritical Fluid Extraction

초임계유체 추출에 의한 생강(Zingiber officinale Roscoe) Oleoresin의 항균활성

  • Lee, Myung-Hee (Processing Technology Research Group, Korea Food Research Institute) ;
  • Lee, Kyoung-Hae (Dept. of Food Science & Biotechnology, Dongnam Health College) ;
  • Kim, Kyung-Tack (Processing Technology Research Group, Korea Food Research Institute) ;
  • Kim, Sung-Soo (Processing Technology Research Group, Korea Food Research Institute)
  • 이명희 (한국식품연구원 공정기술연구단) ;
  • 이경혜 (동남보건대학교 식품생명과학과) ;
  • 김경탁 (한국식품연구원 공정기술연구단) ;
  • 김성수 (한국식품연구원 공정기술연구단)
  • Received : 2012.04.16
  • Accepted : 2012.05.08
  • Published : 2012.06.30
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Abstract

The study indicated that antimicrobial activity about gram positive and gram negative bacteria of ginger-oleoresin(GO) extract with the condition of ethanol and supercritical fluid extractions. As the concentration of extraction increases, the clear zone of GO ethanol extract also increased dependently. This led the antimicrobial activity of gram positive bacteria to take bigger place than gram negative bacteria especially in Listeria monocytogenes. There was a high antimicrobial activity in E-III treatment where the ratio of the ginger powder extract to ethanol extraction was 1:6. It was quite effective to treat the antimicrobial activity of GO ethanol extract under $80^{\circ}C$ and there was not big difference in the intervals which were the extraction time - 1 to 7 hours. The antimicrobial activity of supercritical fluid extract seemed to take the biggest place in Listeria monocytogenes. From the supercritical fluid extract, it was shown the strong ability of antimicrobial activity in the condition with 100 bar $35^{\circ}C$, 250 bar $35^{\circ}C$ and 250 bar $65^{\circ}C$. Furthermore, according to the case of solvent extract, there was not any significant difference in the antimicrobial activity with condition of extraction. However, there was significant antimicrobial activity in E-III treatment of 100 bar and 500 bar of extraction pressure, and $35^{\circ}C$ and $65^{\circ}C$ of extraction temperature.

본 연구에서는 에탄올추출과 초임계추출(SFE) 조건에 따른 생강 oleresin(GO) 추출물의 Gram 양성균(Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes)과 Gram 음성균(Pseudomonas aeruginosa, Escherichia coli)에 대한 항균활성을 살펴보았다. GO 에탄올추출은 추출물의 농도가 증가할수록 clear zone이 비례적으로 증가하였다. 이의 항균성은 Gram 양성균이 Gram 음성균보다 크게 나타났으며, 특히 Listeria monocytogenes에서 항균활성이 크게 나타났다. 생강분말과 에탄올의 추출비율이 1:6인 E-III 처리구가 항균활성이 높았다. GO 에탄올추출물의 항균성은$80^{\circ}C$ 조건에서 처리하는 것이 효과적이었고, 추출시간 (1시간~7시간)은 처리구 간에 큰 차이가 없었다. 초임계추출물에 대한 항균성은 Listeria monocytogenes에서 가장 크게 나타났다. 초임계추출물에서는 100 bar $35^{\circ}C$, 250 bar $35^{\circ}C$, 250 bar $65^{\circ}C$ 조건에서 강한 항균력을 보였다. 그리고 용매추출물의 경우 추출조건에 따라서 항균활성에 큰 차이를 나타내지 않았으나, 초임계추출물의 경우 추출압력 100 bar, 500 bar 처리구와 추출온도 $35^{\circ}C$, $65^{\circ}C$ 처리구에서 항균활성이 크게 나타났다.

Keywords

References

  1. Koo, M.S. and Kim, H.J.: Shiga toxin producing Escherichia coli and foodborne disease. Safe Food, 6(4), 23-28 (2011).
  2. Kim, W.I., Jung, H.M., Kim, S.R., Park, K.H., Kim, B.S, Yun, H.J., Yun, J.C. and Ryu, K.Y.: Growth potential of Escherichia coli O157:H7 in lettuce leaf extract and on lettuce leaf surface. J. Food Hyd. Safety, 26, 296-301 (2011).
  3. Branen, A.L.: Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. JAPCS, 52(1), 59-63 (1975). https://doi.org/10.1007/BF02901825
  4. Brewer, M.S., Sprouls, G.K., Russon, C.: Consumer attitutes toward food safety issues. J. Food Safety, 6, 29-34 (1983).
  5. Kim, D.H. and Lee, Y.C.: Quality changes in minced ginger prepared with frozen ginger during storage. Korean J. Food Sci. Technol., 36, 943-951 (2004).
  6. Sheo, H.J.: The antibacterial action of garlic, onion, ginger and red pepper juice. J. Korean Soc. Food Sci. Nutr., 28, 94-99 (1999).
  7. Kim, J.S., Koh, M.S., Kim, Y.H., Kim, M.K. and Hong, J.S.: Volatile flavor component of korean ginger (Zingiber officinale Roscoe). Korean J. Food Sci. Technol., 23, 141-149 (1991).
  8. Lee, J.Y. and Ahn, M.S.: Changes of antioxidative properties according to the heat- treatment of ginger extracts. Korean J. Soc. Food Sci., 10, 63-70 (1994).
  9. Connell, D.W.: The chemistry of the essential oil and oleoresin of ginger. The flavor industry, 1, 677-693 (1970).
  10. Bhattarai, S., Tran, V.H., Duke, C.C.: The stability of gingerol and shogaol in aqueous solutions. J. Pharm. Sci., 90, 1658-1664 (2001). https://doi.org/10.1002/jps.1116
  11. Alfaro, M.J., Belanger, J.M.R., Padilla, F.C., Pare, J.R.J.: Influence of solvent matrix dielectric properties and applied power on the liquid-phase microwave-assisted processes$(MAP^{TM})^1$ extraction of ginger. Food Res. Int., 36, 499-504 (2003). https://doi.org/10.1016/S0963-9969(02)00198-9
  12. Ziauddin, K.S., Rao, H.S. and Fairoze, N.: Effect of organic acids and spices on quality and self-life of meats at ambient temperature. J. Food Sci. Technol., 33, 255-258 (1996).
  13. Tansey, M.R. and Appleton, J.A.: Inhibition of fungal growth by garlic extract. Mycologia, 7, 397-401 (1978).
  14. Tammura, M.: Extraction of antimicrobial compounds from Allium sativum. Japan Patent 94 192 116 (1994).
  15. Sheo, H.J.: The antibacterial action of garlic, onion, ginger and red pepper juice. J. Korean Soc. Food Sci. Nutr., 28, 94-99 (1999).
  16. Kim, K.J., Do, J.R. and Kim, H.K.: Antimicrobial, antihypertensive and anticancer activities of garlic extracts. Korean J. Food Sci. Technol., 37, 228-232 (2005).
  17. Kim, J.H.: Anti-bacterial action of onion (Allium cepa L.) extracts against oral pathogenic bacteria. J. Nippon Univ. Sch. Dent., 39, 136-141 (1997). https://doi.org/10.2334/josnusd1959.39.136
  18. An, E.Y., Han, J.S. and Shin, D.W.: Growth inhibition of Listeria monocytogenes by pure compound isolated from extract of Morus alba Linne bark. Korean J. Food Sci. Technol., 29, 1236-1240 (1997).
  19. Park, U.Y., Chang, D.S. and Cho, H.R.: Screening of antimicrobial activity for medicinal herb extracts. J. Korean Soc. Food Sci. Nutr., 21, 91-96 (1992).
  20. Kee, J.Y., Min, Y.K. and Kim, H.Y.: Isolation of antimicrobial substance from Schizandra chinensis Baillon and antimicrobial effect. Korean J. Food Sci. Technol., 33, 389-394 (2001).
  21. Ryu, H.Y., Bae, K.H., Kum, E.J., Park, S.J., Lee, B.H. and Sohn, H.Y.: Evaluation for antimicrobial, antioxidants activity of natural spices for fresh-cut yam. J. Life Sci., 17, 652-657 (2007). https://doi.org/10.5352/JLS.2007.17.5.652
  22. Yoon, S.Y., Lee, S.Y., Kim, K.B.W.R., Song, E.J., Leem S.J., Lee, C.J., Park, N.B., Jung, J.Y., Kwak, J.H., Nam, K.W. and Ahnm D.H.: Antimicrobial activity of the Sargassum fulvellum ethanol extract and the effect of temperature and pH on their activity. Korean J. Food Sci. Technol., 42, 155-159 (2010).
  23. Yoo, M.J., Kim, Y.S. and Shin, D.H.: Antibacterial effects of natural essential oils from various spices against Vibrio spices and their volatile constituents. Korean J. Food Sci. Technol., 38, 438-443 (2006).
  24. Lee, S.E., Park, C.G., Cha, M.S., Kim, J.K., Seong, N.S., Bang, K.H. and Bang, J.K.: Antimicrobial activity of essential oils from Mentha arvensis L. var. piperascens Malivaud and Agastache rugosa O. Kuntze on Escherichia coli and Samonella typhimurium. Korean J. Medicinal Crop. Sci., 10, 206-211 (2002).
  25. Lee, S.K.: Antimicrobial activity of Bamboo (Phyllostachys bambusoides) essential oil. J. Food Hyg. Safety, 15, 55-50 (2000).
  26. Kim, S.J., Shin, J.Y., Park, Y.M., Chung, K.M., Lee, J.H. and Kweon, D.H.: Investigation of antimicrobial activity and stability of ethanol extracts of Licorice root (Glycyrrhiza glabra). Korean J. Food Sci. Technol., 38, 241-248 (2006).
  27. Bozin, B., Mimica-Dukic, N., Simin, N. and Anackov, G.: Characterization of the volatile composition of essential oils of some Lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils. J. Agric. Food Chem., 54, 1822-1828 (2006). https://doi.org/10.1021/jf051922u
  28. McHugh, M.A. and Krukonis, V.J.: Supercritical fluid extraction, principle and practice. Butterworths Publishers, London, UK, pp. 181-195 (1986).
  29. Dzievak, J.D.: Innovative separation process finding it's way into the food industry. Food Technol., 40, 66-69 (1986).
  30. Brunner, G.: Gas extraction. Steinkopff Darmstadt Springer. New York. USA, pp 179-192 (1994).
  31. Lee, S.H., Cheon, J.K. and Ju, C.S.: Lipid extraction of sea tangle with supercritical carbon dioxide. Korean J. Food Eng. Prog., 4, 19-24 (2000).
  32. Lee, M.H., Lee, K.H. and Kim, K.T.: Quality Characteristics of Ginger (Zingiber officinale Roscoe) Oleoresin by Supercritical Fluid Extraction. J. Food Hyg. Safety, 26, 36-42 (2011).
  33. Jung, S.H., Chang, K.S. and Ko, K.H.: Physiological effects of curcumin extracted by supercritical fluid from turmeric (Curcuma longa L.). Korean J. Food Sci. Technol., 36, 317- 320 (2004).
  34. Reverchon, E.: Supercritical fluid extraction and fraction-ation of essential oils and related products. J. Supercritical Fluids, 10, 1-37 (1997). https://doi.org/10.1016/S0896-8446(97)00014-4
  35. Woo, G.Y., Kim, K.H., Lee, M.J., Lee, Y.B. and Yoon, J.R.: A comparison of volatile compounds in pine extracts obtained by supercritical fluid extraction with those by simultaneous steam distillation and solvent extraction. Korean J. Food Sci. Technol., 31, 1268-1274 (1999).
  36. Lee, H.C., Seo, H.Y,, Shin, D.B., Park, Y.K., Kim, Y.S., Ji, J.R.. and Choi, H.D.: Supercritical fluid extracted of volatile components from strawberry. Korean J. Food Sci. Technol., 41, 615-621 (2009).
  37. Kim, M.L., Choi, K.H. and Park, C.S.: Growth inhibition of food-borne bacteria by juice and extract of ginger and garlic. J. East Asian Soc. Dietary Life, 10, 160-169 (2000).
  38. Ji, W.D., Jeong, M.S., Chung, H.C., Lee, S.J. and Chung Y.G.: Antimicrobial activity of distilled components of garlic (Allium sativum L.) and Ginger (Zingiber officinale Roscoe). Agric. Chem. Biotechnol., 40, 514-518 (1997).
  39. Chung, C.K., Park, O.K., Yoo, I.J., Park, K.M. and Choi, C.U.: Antimicrobial activity of essential oils of curry spices. Korean J. Food Sci. Technol., 22, 716-719 (1990).
  40. Hargreaves, L.L., Jarvis, B., Rawlinson, A.P. and Wood, J.M.: The british food manufacturing industries research association scientific and technical survey. pp. 88-91 (1975).
  41. Kim, M.L., Choi, K.G. and Park, C.S.: Antibacterial activity of powdered spice against Escherichia coli and Staphylococcus aureus. Korean J. Postharvest Sci. Technol., 7, 124-131 (2000).

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