Applied Microscopy

  • 제42권1호
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  • Pages.9-16
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  • 2012
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
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DOI QR코드

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고양이 삼차신경꼬리핵에서 저역치기계자극수용기 유래 들신경종말의 연접이전종말이 함유하는 신경전달물질에 대한 정량적 분석

Quantitative Analysis of Neurotransmitters in the Endings Presynaptic to Vibrissa Afferent Terminals in the Cat Trigeminal Caudal Nucleus

  • 김윤숙 (경북대학교 치의학전문대학원 구강해부학교실) ;
  • 문철주 (경북대학교 치의학전문대학원 구강해부학교실) ;
  • 조진현 (경북대학교 치의학전문대학원 치과보철학교실) ;
  • 배진영 (경북대학교 치의학전문대학원 구강해부학교실) ;
  • 나연경 (경북대학교 간호대학 기초간호과학교실) ;
  • 복혜정 (부산여자대학교 치위생과) ;
  • 배용철 (경북대학교 치의학전문대학원 구강해부학교실) ;
  • 백상규 (경북대학교 치의학전문대학원 구강해부학교실)
  • Kim, Yun-Sook (Department of Oralanatomy and Neurobiology, Kyungpook National University) ;
  • Mun, Cheol-Ju (Department of Oralanatomy and Neurobiology, Kyungpook National University) ;
  • Cho, Jin-Hyun (Department of Prosthetics, School of Dentistry, Kyungpook National University) ;
  • Bae, Jin-Young (Department of Oralanatomy and Neurobiology, Kyungpook National University) ;
  • Na, Yeon-Kyung (Division of Basic Nursing Science, College of Nursing, Kyungpook National University) ;
  • Bok, Hye-Jeong (Department of Dental Hyegine, Busan Women's College) ;
  • Bae, Yong-Chul (Department of Oralanatomy and Neurobiology, Kyungpook National University) ;
  • Paik, Sang-Kyoo (Department of Oralanatomy and Neurobiology, Kyungpook National University)
  • 투고 : 2012.01.19
  • 심사 : 2012.03.26
  • 발행 : 2012.03.31
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초록

이 연구에서는 삼차신경꼬리핵 제 3~4층에서 저역치기계자극정보를 전달하는 일차들신경섬유의 종말과 연접하는 연접이전종말(presynaptic ending; p-ending)들이 어떤 억제성 신경전달물질을 함유하는 지를 분석하고자 하였다. 이를 위해 전기생리학적으로 동정된 고양이콧수염유래 일차들신경종말을 단일 축삭내 HRP주입법으로 표식하였고, GABA와 glycine에 대한 항혈청으로 포매후금입자면역염색법을 시행한 후, 정량적 분석을 실시하였다. 표식종말과 연접하는 16개 p-ending들 중 8개(50%, 8/16) p-ending들은 GABA만을 함유하였으며, 나머지 8개(50%, 8/16) p-ending들은 GABA와 glycine 모두를 함유하는 집단으로 분류할 수 있었다. 또한, 이 두 집단의 p-ending 사이에는 유의한 평균체적의 차이가 보이지 않았으며, 각 p-ending이 함유하는 GABA와 glycine의 상대적 함량은 서로 달랐다. 이러한 결과들은 삼차신경꼬리핵에서 콧수염유래 일차들신경섬유에 의해 전달되는 저역치기계자극정보는 GABA 및 glycine에 의해 연접이전제어(presynaptic modulation)를 받으며, 그 연접이전제어는 각 일차들신경섬유의 종말마다 다르게 나타날 것 이라는 점을 제시한다.

The goal of this study was to identify neurotransmitters in endings (p-endings) presynaptic to low-threshold mechanoreceptive vibrissa afferents in the laminae III/IV of cat trigeminal caudal nucleus (Vc). Rapidly-adapting vibrissa afferents were intra-axonally labeled after electrophysiological identification, and postembedding immunogold staining with antisera against ${\gamma}$-aminobutyric acid (GABA) and glycine was performed, followed by quantitative ultrastructural analysis of p-endings presynaptic to the labeled vibrissa afferent terminals. Sixteen p-endings, which are presynaptic to the HRP-labeled vibrissa afferent terminals, were analyzed in this study: Eight p-endings (50%, 8/16) were immunopositive to GABA but immunonegative to glycine (GABA+ p-ending), and remaining 8 p-endings (50%, 8/16) exhibited immunoreactivity to both GABA and glycine. Bouton volume of the p-endings was not significantly different between the two groups. However, the p-endings differed from each other in relative content of GABA and glycine. These findings suggest that low-threshold mechanoreceptive information conveyed through vibrissa afferent at Vc is presynaptically modulated by GABA and/or glycine, and that degree of presynaptic modulation may differ among each vibrissa afferent terminal.

키워드

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