Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Antimicrobial and Biogenic Amine-Degrading Activity of Bacillus licheniformis SCK B11 Isolated from Traditionally Fermented Red Pepper Paste

전통고추장에서 유해균 억제 및 Biogenic Amines 분해 능력을 가지는 Bacillus licheniformis SCK B11의 분리

  • Kim, Yong-Sang (Department of Biological Sciences, Chonbuk National University) ;
  • Jeong, Jin-Oh (Department of Biological Sciences, Chonbuk National University) ;
  • Cho, Sung-Ho (Sunchang Research Center for Fermentation Microbes (SRCM)) ;
  • Jeong, Do-Yeon (Sunchang Research Center for Fermentation Microbes (SRCM)) ;
  • Uhm, Tai-Boong (Department of Biological Sciences, Chonbuk National University)
  • 김용상 (전북대학교 자연과학대학 생명과학과) ;
  • 정진오 (전북대학교 자연과학대학 생명과학과) ;
  • 조성호 (순창발효미생물관리센터) ;
  • 정도연 (순창발효미생물관리센터) ;
  • 엄태붕 (전북대학교 자연과학대학 생명과학과)
  • Received : 2012.06.05
  • Accepted : 2012.06.27
  • Published : 2012.06.30
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Abstract

In order to inhibit the growth of pathogens and degrade biogenic amines during the fermentation of soybean products, an isolate with antimicrobial activity against pathogens and biogenic amine-degrading property was obtained from 83 traditionally fermented soybean products. The morphological and biochemical tests and the phylogenetic relationship among 16S rRNA gene sequences indicated that the isolate named as the strain SCK B11 was most closely related to Bacillus licheniformis. The cell-free supernatant of two day cultures was active against several pathogens including Enterococcus faecalis, Listeria monocytosis, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus. PCR analysis was conducted to determine relatedness to antimicrobial lantibiotics and biosurfactants produced by Bacillus spp., but showed negative for the genes encoding surfactin, lichenysin, and lichenicidine. Electron microscopic observation indicated that the antimicrobial agent seemed to attack the membrane of the pathogens, leaving the ghost or shrunken cells. The strain was found to degrade histamine by 72% and tyramine by 66% in the cooked soybean containing 5.3% of biogenic amine over 10 days of fermentation time. The use of selected strain would be a potential control measure in manufacturing traditionally fermented soybean products that are difficult to control pathogens and biogenic amine levels.

장류의 유해균과 biogenic amines 함량을 줄이기 위해 항균작용과 biogenic amines 분해능력이 있는 한 균주를 83종의 전통장류로부터 분리하였다. 형태 및 생화학적 특성, 16S rRNA 유전자 서열 해독 결과 이 균주는 Bacillus licheniformis에 속했으며, B. licheniformis SCK B11로 명명되었다. SCK B11을 이틀간 배양한 후의 원심분리 상층액은 유해 균주들인 Enterococcus faecalis, Listeria monocytogenes, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus aureus, Aspergillus flavus에 대해 균 증식 저지력을 보였다. 이 균주의 항균 특성을 확인하기 위해 B. licheniformis의 항균물질로 알려진 surfactin, lichenysin, lichenicidine 합성 유전자들에 대한 PCR을 수행한 결과 모두 음성으로 나와 이 항균 물질은 기존에 보고된 물질들과는 다름을 보였다. 또한 전자 현미경 사진 결과 이 항균 물질은 유해균들의 세포막 손상을 야기하는 것이 관찰되었다. 이 균주는 각각 5.3%의 histamine과 tyramine이 함유된 삶은 콩에서 10일의 발효 기간 동안 histamine 함량의 72%, tyramine 함량의 66%를 분해하는 것으로 나타났다. SCK B11의 항균 및 biogenic amine 분해 특성을 고려할 때, 공정상 필연적으로 유해균 증식 및 biogenic amine 생성 문제를 지닌 전통 장류 발효 과정에서 이 균주는 이 문제점들을 해결할 수 있을 것으로 보인다.

Keywords

References

  1. Altayar, M. and Sutherland, A.D. 2005. Bacillus cereus is common in the environment but emetic toxin producing isolates are rare. J. Appl. Microbiol. 100, 7-14.
  2. Apetroaie, C., Andersson, M.A., Sproer, C., Tsitko, I., Shaheen, R., Jaaskelainen, E.L., Wijnands, L.M., Heikkila, R., and Salkinoinoja- Salonen, M.S. 2005. Cereulide-producing strains of Bacillus show diversity. Arch. Microbiol. 184, 141-151. https://doi.org/10.1007/s00203-005-0032-1
  3. Bermudez, R., Lorenzo, J.M., Fonseca, S., Franco, I., and Carballo, J. 2012. Strains of Staphylococcus and Bacillus isolated from traditional sausages as producers of biogenic amines. Front. Microbiol. 3, 1-6.
  4. Birgit, M.P., Dietrich, R., Nibler, B., Martlbauer, E., and Scherer, S. 1999. The hemolytic enterotoxin HBL is broadly distributed among species of the Bacillus cereus group. Appl. Environ. Microbiol. 65, 5436-5442.
  5. Chakravorty, S., Helb, D., Burday, M., Connell, N., and Alland, D. 2007. A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria. J. Microbiol. Methods 69, 330-339. https://doi.org/10.1016/j.mimet.2007.02.005
  6. Cho, T.Y., Hahn, G.H., Bahn, K.N., Son, Y.W., Jang, M.R., Lee, S.H., Kim, D.B., and Kim, S.B. 2006. Evaluation of biogenic amines in Korean commercial fermented foods. Korean J. Food Sci. Technol. 38, 730-737.
  7. Ferencik, M. 1970. Formation of histamine during bacterial decarboxylation of histidine in the flesh of some marine fishes. J. Hyg. Epidemiol. Microbiol. Immunol. 14, 52-60.
  8. Fitch, W.M. 1971. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool. 20, 406-416. https://doi.org/10.2307/2412116
  9. Granum, P.E. and Lund, T. 1997. Bacillus cereus and its food poisoning toxins. FEMS Microbiol. Lett. 157, 223-228. https://doi.org/10.1111/j.1574-6968.1997.tb12776.x
  10. Guinebretiere, M.H., Broussolle, V., and Nguyen-The, T. 2002. Enterotoxigenic profiles of food-poisoning and food-borne Bacillus cereus strains. J. Clin. Microbiol. 40, 3053-3056. https://doi.org/10.1128/JCM.40.8.3053-3056.2002
  11. Havelka, B. 1967. Role of the Hafnia bacteria in the rise of histamine in tuna fish meat. Cesk. Hyg. 12, 343-352.
  12. Kawabata, T., Ishizaka, K., Mura, T., and Sasaki, T. 1956. Studies on the food poisoning associated with putrefaction of marine products. VII. Bull. Jpn. Soc. Sci. Fish. 22, 41-47. https://doi.org/10.2331/suisan.22.41
  13. Kim, Y.S., Kim, M.C., Kwon, S.W., Kim, S.J., Park, I.C., Ka, J.O., and Weon, H.Y. 2011. Analysis of bacterial communities in Meju, a Korean traditional fermented soybean bricks, by cultivation-based and pyrosequencing methods. J. Microbiol. 49, 340-348. https://doi.org/10.1007/s12275-011-0302-3
  14. Kim, Y.S., Yun, S.H., Jeong, D.Y., Hahn, K.S., and Uhm, T.B. 2010. Isolation of Bacillus licheniformis producing antimicrobial agents against Bacillus cereus and its properties. Korean J. Microbiol. 46, 270-277.
  15. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16, 111-120. https://doi.org/10.1007/BF01731581
  16. Lerke, P.A., Werner, S.B., Taylor, S.L., and Guthertz, L.S. 1978. Scombroid poisoning. Report of an outbreak. West J. Med. 129, 381- 386.
  17. Lindback, T., Fagerlund, A., Rodland, M.S., and Granum, P.E. 2004. Characterization of the Bacillus cereus Nhe enterotoxin. Microbiology 150, 3959-3967. https://doi.org/10.1099/mic.0.27359-0
  18. Naila, A., Flint, S., Fletcher, G., Bremer, P., and Meerdink, G. 2010. Control of biogenic amines in food-existing and emerging approaches. J. Food Sci. 75, 139-150. https://doi.org/10.1111/j.1750-3841.2010.01774.x
  19. Ngamwongsatit, P., Buasri, W., Pianariyanon, P., Pulsrikarn, C., Ohba, M., Assavanig, A., and Panbangred, W. 2008. Broad distribution of enterotoxin genes among Bacillus thuringiensis and Bacillus cereus as shown by novel primers. Int. J. Food Microbiol. 121, 352-356. https://doi.org/10.1016/j.ijfoodmicro.2007.11.013
  20. Omura, V., Price, R.J., and Olcott, H.S. 1978. Histamine forming bacteria isolated from spoiled skipjack tuna and jack mackerel. J. Food Sci. 43, 1779-1787. https://doi.org/10.1111/j.1365-2621.1978.tb07412.x
  21. Ouhib, Q., Clavel, T., and Schmitt, P. 2006. The production of Bacillus cereus enterotoxins is influenced by carbohydrate and growth rate. Curr. Microbiol. 53, 222-226. https://doi.org/10.1007/s00284-006-0094-6
  22. Schoeni, J.L. and Wong, A.C. 2005. Bacillus cereus food poisoning and its toxins. J. Food Prot. 68, 636-648. https://doi.org/10.4315/0362-028X-68.3.636
  23. Shalaby, A.R. 1996. Significance of biogenic amines to food safety and human health. Food Res. Int. 29, 675-690. https://doi.org/10.1016/S0963-9969(96)00066-X
  24. Swofford, D.L. 1998. PAUP. Phylogenetic Analysis using Parsimony. 4.0 ed. Sinauer Associates, Sunderland, MA, USA.
  25. Taylor, S.L and Speckhard, M.W. 1983. Isolation of histamineproducing bacteria from frozen tuna. Mar. Fish. Rev. 45, 35-39.
  26. Taylor, S.L., Guthertz, L.S., Leatherwood, M., and Lieber, E.R. 1979. Histamine production by Klebsiella pneumoniae and an incident of scombroid fish poisoning. Appl. Environ. Microbiol. 37, 274-278.
  27. Ten Brink, B., Damink, C., Joosten, H.M., and Huis in't Veld, J.H. 1990. Occurrence and formation of biologically active amines in foods. Int. J. Food Microbiol. 11, 73-84. https://doi.org/10.1016/0168-1605(90)90040-C
  28. Thompson, J.D., Higgins, D.G., and Gibson, T.J. 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighing position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680. https://doi.org/10.1093/nar/22.22.4673
  29. Zhang, Z., Schwarz, S., Wagner, L., and Miller, W. 2000. A greedy algorithm for aligning DNA sequences. J. Comput. Biol. 7, 203-214. https://doi.org/10.1089/10665270050081478

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