Journal of Microbiology and Biotechnology

  • 제22권6호
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  • Pages.736-741
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  • 2012
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Negative Role of wblA in Response to Oxidative Stress in Streptomyces coelicolor

  • Kim, Jin-Su (Department of Biological Engineering, Inha University) ;
  • Lee, Han-Na (Department of Biological Engineering, Inha University) ;
  • Kim, Pil (Department of Biotechnology, The Catholic University of Korea) ;
  • Lee, Heung-Shick (Department of Biotechnology and Bioinformatics, Korea University) ;
  • Kim, Eung-Soo (Department of Biological Engineering, Inha University)
  • 투고 : 2011.12.16
  • 심사 : 2012.01.25
  • 발행 : 2012.06.28
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초록

In this study, we analyzed the oxidative stress response of wblA ($\underline{w}$hi$\underline{B}$-$\underline{l}$ike gene $\underline{A}$, SCO3579), which was previously shown to be a global antibiotic down-regulator in Streptomyces coelicolor. Ever since a WblA ortholog named WhcA in Corynebacterium glutamicum was found to play a negative role in the oxidative stress response, S. coelicolor wblA has been proposed to have a similar effect. A wblA-deletion mutant exhibited a less sensitive response to oxidative stress induced by diamide present in solid plate culture. Using real-time RT-PCR analysis, we also compared the transcription levels of oxidative stress-related genes, including sodF, sodF2, sodN, trxB, and trxB2, between S. coelicolor wild type and a wblA-deletion mutant in the presence or absence of oxidative stress. Target genes were expressed higher in the wblA-deletion mutant compared with wild type, both in the absence and presence of oxidative stress. Moreover, expression of these target genes in S. coelicolor wild type was stimulated only in the presence of oxidative stress, suggesting that WblA plays a negative role in the oxidative stress response of S. coelicolor, similar to that of C. glutamicum WhcA, through the transcriptional regulation of oxidative stress-related genes.

키워드

참고문헌

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피인용 문헌

  1. A WblA-Binding Protein, SpiA, Involved in Streptomyces Oxidative Stress Response vol.23, pp.10, 2012, https://doi.org/10.4014/jmb.1306.06032
  2. Identification and Biotechnological Application of Novel Regulatory Genes Involved in Streptomyces Polyketide Overproduction through Reverse Engineering Strategy vol.2013, pp.None, 2013, https://doi.org/10.1155/2013/549737
  3. An overview on transcriptional regulators in Streptomyces vol.1849, pp.8, 2012, https://doi.org/10.1016/j.bbagrm.2015.06.007
  4. Streptomyces tsukubaensis as a new model for carbon repression: transcriptomic response to tacrolimus repressing carbon sources vol.101, pp.22, 2012, https://doi.org/10.1007/s00253-017-8545-5
  5. ­Genomic data mining of the marine actinobacteria Streptomyces sp. H-KF8 unveils insights into multi-stress related genes and metabolic pathways involved in antimicrobial synthesis vol.5, pp.None, 2012, https://doi.org/10.7717/peerj.2912
  6. In conditions of over-expression, WblI, a WhiB-like transcriptional regulator, has a positive impact on the weak antibiotic production of Streptomyces lividans TK24 vol.12, pp.3, 2012, https://doi.org/10.1371/journal.pone.0174781
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  8. Rational engineering of Streptomyces albus J1074 for the overexpression of secondary metabolite gene clusters vol.17, pp.None, 2018, https://doi.org/10.1186/s12934-018-0874-2
  9. WblA, a global regulator of antibiotic biosynthesis in Streptomyces vol.48, pp.3, 2012, https://doi.org/10.1093/jimb/kuab007
  10. Activation of Cryptic Antibiotic Biosynthetic Gene Clusters Guided by RNA-seq Data from Both Streptomyces ansochromogenes and ΔwblA vol.10, pp.9, 2012, https://doi.org/10.3390/antibiotics10091097
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