Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Factors Regulating Escherichia coli 2,3-Butanediol Production by Continuous Culture and Metabolic Flux Analysis

  • Lu, Mingshou (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Lee, Soo-Jin (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Kim, Bo-Rim (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Park, Chang-Hun (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Oh, Min-Kyu (Department of Chemical and Biological Engineering, Korea University) ;
  • Park, Kyung-Moon (Department of Biological and Chemical Engineering, Hongik University) ;
  • Lee, Sang-Yup (College of Science and Bioengineering, KAIST) ;
  • Lee, Jin-Won (Department of Chemical and Biomolecular Engineering, Sogang University)
  • Received : 2011.12.08
  • Accepted : 2012.01.14
  • Published : 2012.05.28
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Abstract

2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.

Keywords

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