Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Monitoring of Horizontal Gene Transfer from Agricultural Microorganisms to Soil Bacteria and Analysis of Microbial Community in Soils

  • Kim, Sung-Eun (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology) ;
  • Moon, Jae-Sun (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, Won-Sik (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Sang-Han (Department of Food Science and Technology, Kyungpook National University) ;
  • Kim, Sung-Uk (Division of Biosystems Research, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2011.10.20
  • Accepted : 2011.12.02
  • Published : 2012.04.28
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Abstract

To investigate the possibility of horizontal gene transfer between agricultural microorganisms and soil microorganisms in the environment, Bacillus subtilis KB producing iturin and the PGPR recombinant strain Pseudomonas fluorescens MX1 were used as model microorganisms. The soil samples of cucumber or tomato plants cultivated in pots and the greenhouse for a six month period were investigated by PCR, real-time PCR, Southern hybridization, and terminal restriction fragment length polymorphism (T-RFLP) fingerprinting. Our data from Southern blotting and T-RFLP patterns suggest that the model bacteria do not give significant impacts on the other bacteria in the pots and greenhouse during cultivation.

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References

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