Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Application of Alkaliphilic Biofilm-Forming Bacteria to Improve Compressive Strength of Cement-Sand Mortar

  • Park, Sung-Jin (School of Life Sciences and Institute for Microorganisms, Kyungpook National University) ;
  • Chun, Woo-Young (School of Architecture and Architectural Engineering, Kyungpook National University) ;
  • Kim, Wha-Jung (School of Architecture and Architectural Engineering, Kyungpook National University) ;
  • Ghim, Sa-Youl (School of Life Sciences and Institute for Microorganisms, Kyungpook National University)
  • Received : 2011.10.04
  • Accepted : 2011.11.15
  • Published : 2012.03.28
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Abstract

The application of microorganisms in the field of construction material is rapidly increasing worldwide; however, almost all studies that were investigated were bacterial sources with mineral-producing activity and not with organic substances. The difference in the efficiency of using bacteria as an organic agent is that it could improve the durability of cement material. This study aimed to assess the use of biofilm-forming microorganisms as binding agents to increase the compressive strength of cement-sand material. We isolated 13 alkaliphilic biofilmforming bacteria (ABB) from a cement tetrapod block in the West Sea, Korea. Using 16S RNA sequence analysis, the ABB were partially identified as Bacillus algicola KNUC501 and Exiguobacterium marinum KNUC513. KNUC513 was selected for further study following analysis of pH and biofilm formation. Cement-sand mortar cubes containing KNUC513 exhibited greater compressive strength than mineral-forming bacteria (Sporosarcina pasteurii and Arthrobacter crystallopoietes KNUC403). To determine the biofilm effect, Dnase I was used to suppress the biofilm formation of KNUC513. Field emission scanning electron microscopy image revealed the direct involvement of organic-inorganic substance in cement-sand mortar.

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References

  1. Achal, V., A. Mukherjee, P. C. Basu, and M. S. Reddy. 2009. Strain improvement of Sporosarcina pasteurii for enhanced urease and calcite production. J. Ind. Microbiol. Biotechnol. 36: 981-988. https://doi.org/10.1007/s10295-009-0578-z
  2. American Public Health Association (APHA). 1989. Standard Methods for the Examination of Water and Wastewater, 17th Ed. American Public Health Association, Washington, DC.
  3. Bang, S. S., J. K. Galinat, and V. Ramakrishnan. 2001. Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii. Enzyme Microb. Technol. 28: 404-409. https://doi.org/10.1016/S0141-0229(00)00348-3
  4. Burton, E., N. Yakandawala, K. Lovetri, and M. S. Madhyastha. 2007. A microplate spectrofluorometric assay for bacterial biofilms. J. Ind. Microbiol. Biotechnol. 34: 1-4.
  5. Chiara, B., G. Alessandro, M. Giorgio, R. Mila, T. Elena, and P. Brunella. 2007. Bacillus subtilis gene cluster involved in calcium carbonate biomineralization. J. Bacteriol. 189: 228-235. https://doi.org/10.1128/JB.01450-06
  6. De Muynck, W., D. Debrouwer, N. De Belie, and W. Verstraete. 2008. Bacterial carbonate precipitation improves the durability of cementitious materials. Cem. Concr. Res. 38: 1005-1014. https://doi.org/10.1016/j.cemconres.2008.03.005
  7. De Muynck, W., D. Debrouwer, N. De Belie, and W. Verstraete. 2010. Microbial carbonate precipitation in construction materials: A review. Ecol. Eng. 36: 118-136. https://doi.org/10.1016/j.ecoleng.2009.02.006
  8. Douglas, S. and T. J. Beveridge. 1998. Mineral formation by bacteria in natural microbial communities. FEMS Microbiol. Ecol. 26: 79-88. https://doi.org/10.1111/j.1574-6941.1998.tb00494.x
  9. Edmund, B. 2003. Biomineralization of unicellular organisms: an unusual membrane biochemistry for the production of inorganic nano- and microstructures. Angew. Chem. Int. Ed. 42: 614-641. https://doi.org/10.1002/anie.200390176
  10. Ghosh, P., S. Mandal, B. D. Chattopadhyay, and S. Pal. 2005. Use of microorganism to improve the strength of cement mortar. Cem. Concr. Res. 35: 1980-1983. https://doi.org/10.1016/j.cemconres.2005.03.005
  11. Ghosh, S., M. Biswas, B. D. Chattopadhya, and S. Mandal. 2009. Microbial activity on the microstructure of bacteria modified mortar. Cem. Concr. Compos. 31: 93-98. https://doi.org/10.1016/j.cemconcomp.2009.01.001
  12. Hammes, F., N. Boon, J. de Villiers, W. Verstraete, and S. D. Siciliano. 2003. Strain-specific ureolytic microbial calcium carbonate precipitation. Appl. Environ. Microbiol. 69: 4901-4909. https://doi.org/10.1128/AEM.69.8.4901-4909.2003
  13. Knorre, H. and W. Krumbein. 2000. Bacterial calcification, pp. 25-31. In R. E. Riding and S. M Awramik (eds.). Microbial Sediments. Springer-Verlag, Berlin, Germany.
  14. Le Metayer-Levrel, G., S. Castanier, G. Orial, J.-F. Loubiere, and J.-P. Perthuisot. 1999. Applications of bacterial carbonatogenesis to the protection and regeneration of limestones in buildings and historic patrimony. Sediment. Geol. 126: 25-34. https://doi.org/10.1016/S0037-0738(99)00029-9
  15. Myszka, K. and K. Czaczyk. 2009. Characterization of adhesive exopolysaccharide (EPS) produced by Pseudomonas aeruginosa under starvation conditions. Curr. Microbiol. 58: 541-546. https://doi.org/10.1007/s00284-009-9365-3
  16. Park, S. J., Y. M. Park, W. Y. Chun, W. J. Kim, and S. Y. Ghim. 2010. Calcite-forming bacteria for compressive strength improvement in mortar. J. Microbiol. Biotechnol. 20: 782-788.
  17. Park, S. J., N. Y. Lee, W. J. Kim, and S. Y. Ghim. 2010. Application of bacteria isolated from dok-do for improving compressive strength and crack remediation of cement-sand mortar. Kor. J. Microbiol. Biotechnol. 38: 216-212.
  18. Ramachandran, S. K., V. Ramakrishnan, and S. S. Bang. 2001. Remediation of concrete using micro-organisms. ACI Mater. J. 98: 3-9.
  19. Stepanovic, S., D. Vukovic, I. Dakic, B. Savic, and M. Svabic-Viahovic. 2000. A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J. Microbiol. Methods 40: 175-179. https://doi.org/10.1016/S0167-7012(00)00122-6
  20. Stocks-Fischer, S., J. K. Galinat, and S. S. Bang. 1999. Microbiological precipitation of $CaCO_3$. Soil Biol. Biochem. 31: 1563-1571. https://doi.org/10.1016/S0038-0717(99)00082-6
  21. Tiano, P., L. Biagiotti, and G. Mastromei. 1999. Bacterial biomediated calcite precipitation for monumental stones conservation: Methods of evaluation. J. Microbiol. Methods 36: 139-145. https://doi.org/10.1016/S0167-7012(99)00019-6
  22. Tiano, P., E. Cantisani, I. Sutherland, and J. M. Paget. 2006. Biomediated reinforcement of weathered calcareous stones. J. Cult. Herit. 7: 49-55. https://doi.org/10.1016/j.culher.2005.10.003
  23. Whitchurch, C. B., T. Tolker-Nielsen, P. C. Ragas, and J. S. Mattick. 2002. Extracellular DNA required for bacterial biofilm formation. Science 295: 1487. https://doi.org/10.1126/science.295.5559.1487

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