Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Survival and Performance of Two Cellulose-Degrading Microbial Systems Inoculated into Wheat Straw-Amended Soil

  • Li, Peipei (College of Agronomy and Biotechnology, China Agricultural University) ;
  • Zhang, Dongdong (College of Agronomy and Biotechnology, China Agricultural University) ;
  • Wang, Xiaojuan (College of Agronomy and Biotechnology, China Agricultural University) ;
  • Wang, Xiaofen (College of Agronomy and Biotechnology, China Agricultural University) ;
  • Cui, Zongjun (College of Agronomy and Biotechnology, China Agricultural University)
  • Received : 2011.02.16
  • Accepted : 2011.09.20
  • Published : 2012.01.28
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Abstract

A cellulose-degrading composite microbial system containing a mixture of microbes was previously shown to demonstrate a high straw-degrading capacity. To estimate its potential utilization as an inoculant to accelerate straw biodegradation after returning straw to the field, two cellulose-degrading composite microbial systems named ADS3 and WSD5 were inoculated into wheat straw-amended soil in the laboratory. The microbial survival of the inoculant was confirmed by a denaturing gradient gel electrophoresis (DGGE) analysis, whereas the enhancement of straw degradation in soil was assessed by measuring the mineralization of the soil organic matter and the soil cellulase activity. The results indicated that most of the DGGE bands from ADS3 were detected after inoculation into straw-amended autoclaved soil, yet only certain bands from ADS3 and WSD5 were detected after inoculation into straw-amended non-autoclaved soil during five weeks of incubation; some bands were detected during the first two weeks after inoculation, and then disappeared in later stages. Organic matter mineralization was significantly higher in the soil inoculants ADS3 and WSD5 than in the uninoculated controls during the first week, yet the enhanced degradation did not persist during the subsequent incubation. Similar to the increase in soil organic matter, the cellulase activity also increased during the first week in the ADS3 and WSD5 treatments, yet decreased during the remainder of the incubation period. Thus, it was concluded that, although the survival and performance of the two inoculants did not persist in the soil, a significant enhancement of degradation was present during the early stage of incubation.

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References

  1. Abdulla, H. M. 2007. Enhancement of rice straw composting by lignocellulolytic actinomycete strains. Int. J. Agric. Biol. 9: 106-109.
  2. Cai, J. and S. Alimujiang. 2009. Kinetic analysis of wheat straw oxidative pyrolysis using thermogravimetric analysis: Statistical description and isoconversional kinetic analysis. Ind. Eng. Chem. Res. 48: 619-624. https://doi.org/10.1021/ie801299z
  3. Cocolin, L., L. F. Bisson, and D. A. Mills. 2000. Direct profiling of the yeast dynamics in wine fermentations. FEMS Microbiol. Lett. 189: 81-87. https://doi.org/10.1111/j.1574-6968.2000.tb09210.x
  4. Dejonghe, W., N. Boon, D. Seghers, E. M. Top, and W. Verstraete. 2001. Bioaugmentation of soils by increasing microbial richness: Missing links. Environ. Microbiol. 3: 649-657. https://doi.org/10.1046/j.1462-2920.2001.00236.x
  5. Guo, P., W. Zhu, H. Wang, Y. C. Lü, X. F. Wang, D. Zheng, and Z. J. Cui. 2010. Functional characteristics and diversity of a novel lignocelluloses degrading composite microbial system with high xylanase activity. J. Microbiol. Biotechnol. 20: 254-264.
  6. Halsall, D. M. and A. H. Gibson. 1986. Comparison of two Cellulomonas strains and their interaction with Azospirillum brasilense in degradation of wheat straw and associated nitrogen fixation. Appl. Environ. Microbiol. 51: 855-861.
  7. Halvorson, A. D., B. J. Wienhold, and A. L. Black. 2002. Tillage, nitrogen and cropping system effects on soil carbon sequestration. Soil Sci. Soc. Am. J. 66: 906-912. https://doi.org/10.2136/sssaj2002.9060
  8. Haruta, S., Z. Cui, Z. Huang, M. Li, M. Ishii, and Y. Igarashi. 2002. Construction of a stable microbial community with high cellulose-degradation ability. Appl. Microbiol. Biotechnol. 59: 529-534. https://doi.org/10.1007/s00253-002-1026-4
  9. Ho, W. C. and W. H. Ko. 1985. Soil microbiostasis: Effects of environmental and edaphic factors. Soil Biol. Biochem. 17: 167-170. https://doi.org/10.1016/0038-0717(85)90110-5
  10. Jeanmougin, F., J. D. Thompson, M. Gouyg, D. G. Higgins, and T. J. Gibson. 1998. Multiple sequence alignment with Clustal X. Trends Biochem. Sci. 23: 403-405. https://doi.org/10.1016/S0968-0004(98)01285-7
  11. Joshi, S. R., G. D. Sharma, and R. R. Mishra. 1993. Microbial enzyme activities related to litter decomposition near a highway in a sub tropical forest of north east India. Soil Biol. Biochem. 22: 51-55.
  12. Juhanson, J., J. Truu, E. Heinaru, and A. Heinaru. 2009. Survival and catabolic performance of introduced Pseudomonas strains during phytoremediation and bioaugmentation experiment. FEMS Microbiol. Ecol. 70: 446-455. https://doi.org/10.1111/j.1574-6941.2009.00754.x
  13. Kato, S., S. Haruta, Z. J. Cui, M. Ishii, and Y. Igarashi. 2004. Effective cellulose degradation by mixed-culture system composed of a cellulolytic Clostridia and aerobic non-cellulolytic bacteria. FEMS Microbiol. Ecol. 51: 133-142. https://doi.org/10.1016/j.femsec.2004.07.015
  14. Kato, S., S. Haruta, Z. J. Cui, M. Ishii, and Y. Igarashi. 2005. Stable coexistence of five bacterial strains as a cellulosedegrading community. Appl. Environ. Microbiol. 71: 7099-7106. https://doi.org/10.1128/AEM.71.11.7099-7106.2005
  15. Li, P. P., X. J. Wang, X. F. Yuan, X. F. Wang, Y. Z. Cao, and Z. J. Cui. 2011. Screening of a composite microbial system and its characteristics of wheat straw degradation. Agric. Sci. China 10: 1586-1594. https://doi.org/10.1016/S1671-2927(11)60155-7
  16. Muyzer, G., E. C. de Waal, and A. G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reactionamplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59: 695-700.
  17. Pandya, U. and M. Saraf. 2010. Application of fungi as a biocontrol agent and their biofertilizer potential in agriculture. J. Adv. Dev. Res. 1: 90-99.
  18. Ruberto, L., S. C. Vazquez, and W. P. Mac Cormack. 2003. Effectiveness of the natural bacterial flora, biostimulation and bioaugmentation on the bioremediation of a hydrocarbon contaminated antarctic soil. Int. Biodeter. Biodegrad. 52: 115-125. https://doi.org/10.1016/S0964-8305(03)00048-9
  19. Sagova, M. M., L. Cermak, J. Novotna, K. Plhackova, J. Forstova, and J. Kopecky. 2008. Innovative methods for soil DNA purification tested in soils of widely differing characteristics. Appl. Environ. Microbiol. 74: 2902-2907. https://doi.org/10.1128/AEM.02161-07
  20. Saffigna, P. G., D. S. Powlson, P. C. Brookes, and G. A. Thomas. 1989. Influence of sorghum residues and tillage on soil organic matter and soil microbial biomass in an Australian Vertisol. Soil Biol. Biochem. 21: 759-765. https://doi.org/10.1016/0038-0717(89)90167-3
  21. Singer, A. C., D. Smith, W. A. Jury, K. Hathuc, and D. E. Crowley. 2003. Impact of the plant rhizosphere and augmentation on remediation of polychlorinated biphenyl contaminated soil. Environ. Toxicol. Chem. 22: 1998-2004. https://doi.org/10.1897/02-471
  22. Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software Version 4.0. Mol. Biol. Evol. 24: 1596-1599. https://doi.org/10.1093/molbev/msm092
  23. van Veen, J. A., L. S. van Overbeek, and J. D. van Elsas. 1997. Fate and activity of microorganisms introduced into soil. Microbiol. Mol. Biol. Rev. 61: 121-135.
  24. Wang, J. G. and L. R. Bakken. 1997. Competition for nitrogen during decomposition of plant residues: Effect of spatial placement of N-rich and N-poor plant residues. Soil Biol. Biochem. 29: 153-162. https://doi.org/10.1016/S0038-0717(96)00291-X
  25. Wang, X. J., X. F. Yuan, H. Wang, J. J. Li, X. F. Wang, and Z. J. Cui. 2011. Characteristics and community diversity of a wheat straw-colonizing microbial community. Afr. J. Biotechnol. 40: 7853-7861.
  26. Yadav, K. S., M. M. Mishra, and K. K. Kapoor. 1982. The effect of fungal inoculation on composting. Agric. Wastes 4: 329-333. https://doi.org/10.1016/0141-4607(82)90029-4
  27. Zhao, Y., W. Li, Z. Zhou, L. Wang, Y. Pan, and L. P. Zhao. 2005. Dynamics of microbial community structure and cellulolytic activity in agricultural soil amended with two biofertilizers. Eur. J. Soil Biol. 41: 21-29. https://doi.org/10.1016/j.ejsobi.2005.03.002

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