Journal of Microbiology and Biotechnology

  • 제22권1호
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  • Pages.46-49
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  • 2012
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Identification of the Fur-Binding Site in Regulatory Region of the Vulnibactin-Receptor Gene in Vibrio vulnificus

  • Lee, Hyun-Jung (Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health) ;
  • Lee, Kyu-Ho (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
  • 투고 : 2011.08.17
  • 심사 : 2011.09.19
  • 발행 : 2012.01.28
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초록

The Vibrio vulnificus vuuA gene, of which expression is repressed by a complex of iron and ferric uptake regulator (Fur), was characterized to localize the Fur-binding site in its upstream regulatory region. In silico analysis suggested the presence of two possible Fur-binding sites; one is a classical Fur-box and the other is a previously reported distinct Fur-binding site. Site-directed mutagenesis and DNase I protection assays revealed the binding site for the iron-Fur complex, which includes an extended inverted repeat containing a homologous sequence to the classical Fur-box.

키워드

참고문헌

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피인용 문헌

  1. Regulation of haemolysin (VvhA) production by ferric uptake regulator (Fur) in Vibrio vulnificus: repression of vvhA transcription by Fur and proteolysis of VvhA by Fur‐repressive exoproteases vol.88, pp.4, 2013, https://doi.org/10.1111/mmi.12224
  2. Multiple High-affinity Iron-uptake Systems of Vibrio vulnificus vol.43, pp.3, 2013, https://doi.org/10.4167/jbv.2013.43.3.168
  3. Vibrio vulnificus Secretes an Insulin-degrading Enzyme That Promotes Bacterial Proliferation in Vivo vol.290, pp.30, 2012, https://doi.org/10.1074/jbc.m115.656306
  4. Regulation of iron-uptake systems in Vibrio vulnificus, a ferrophilic bacterium vol.4, pp.2, 2012, https://doi.org/10.30579/mbse.2021.4.2.69