Journal of Radiation Protection and Research

The Korean Association for Radiation Protection (대한방사선방어학회)
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THE SHORT-TERM EFFECTS OF LOW-DOSE-RATE RADIATION ON EL4 LYMPHOMA CELL

  • Bong, Jin-Jong (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd) ;
  • Kang, Yu-Mi (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd) ;
  • Shin, Suk-Chul (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd) ;
  • Choi, Moo-Hyun (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd) ;
  • Choi, Seung-Jin (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd) ;
  • Lee, Kyung-Mi (Global Research Lab, BAERI Institute, Department of Biochemistry and Molecular Biology, Korea University College of Medicine) ;
  • Kim, Hee-Sun (Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Ltd)
  • Received : 2012.03.08
  • Accepted : 2012.04.14
  • Published : 2012.06.30
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Abstract

To determine the biological effects of low-dose-rate radiation ($^{137}Cs$, 2.95 mGy/h) on EL4 lymphoma cells during 24 h, we investigated the expression of genes related to apoptosis, cell cycle arrest, DNA repair, iron transport, and ribonucleotide reductase. EL4 cells were continuously exposed to low-dose-rate radiation (total dose: 70.8 mGy) for 24 h. We analyzed cell proliferation and apoptosis by trypan blue exclusion and flow cytometry, gene expression by real-time PCR, and protein levels with the apoptosis ELISA kit. Apoptosis increased in the Low-dose-rate irradiated cells, but cell number did not differ between non- (Non-IR) and Low-dose-rate irradiated (LDR-IR) cells. In concordance with apoptotic rate, the transcriptional activity of ATM, p53, p21, and Parp was upregulated in the LDR-IR cells. Similarly, Phospho-p53 (Ser15), cleaved caspase 3 (Asp175), and cleaved Parp (Asp214) expression was upregulated in the LDR-IR cells. No difference was observed in the mRNA expression of DNA repair-related genes (Msh2, Msh3, Wrn, Lig4, Neil3, ERCC8, and ERCC6) between Non-IR and LDR-IR cells. Interestingly, the mRNA of Trfc was upregulated in the LDR-IR cells. Therefore, we suggest that short-term Low-dose-rate radiation activates apoptosis in EL4 lymphoma cells.

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References

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