Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of G9 genotype porcine rotavirus isolated in Korea

국내 양돈장에서 분리한 G9형 돼지로타바이러스의 특성

  • Received : 2012.04.20
  • Accepted : 2012.05.30
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Porcine rotaviruses are the most common causes of viral gastroenteritis in piglets around the world. The major G genotypes of porcine rotaviruses causing diarrhea were G4, G5 and G11 genotypes. Recently, G9 genotype rotaviruses were problemed at swine farms and frequently recognized from diarrheic piglets. In this study, a porcine rotavirus (PoRV-1) was isolated from piglet showing diarrhea using MA104 cells and confirmed as rotavirus by electron microscopy, genomic RNA electropherotyping and indirect immunofluorescence antibody tests. The nucleotide sequence of the VP7 gene of PoRV-1 was determined and compared with those of other genotype rotavirus strains from other parts of the world. Also, the nucleotide sequences of VP4, VP6 and NSP4 genes of PoRV-1 were determined and compared with those of other rotavirus strains from other countries. The results showed that the PoRV-1 isolate belonged to the G9 genotype and the P, I and E genotypes of PoRV-1 were P[23], I5 and E1, respectively. The Korean G9 PoRV-1 isolate and its nucleotide sequence data would be usefully used for the development of porcine rotavirus vaccines in near future.

Keywords

References

  1. Bellinzoni RB, Mattion NM, Matson DO, Blackhall J, La Torre JL, Scodeller EA, Urasawa S, Taniguchi K, Estes MK. 1990. Porcine rotaviruses antigenically related to human rotavirus serotypes 1 and 2. J Clin MIcrobiol 28: 633- 636.
  2. Chen DY, Estes MK, Ramig RF. 1992. Specific interactions between rotavirus outer capsid proteins VP4 and VP7 determine expression of a cross-reactive, neutralizing VP4- specific epitope. J Virol 66: 432-439.
  3. Clark HF, Hoshino Y, Bell LM, Groff J, Hess G, Bachman P, Offit PA. 1987. Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol 25: 1757-1762.
  4. Clark HF, Lawley DA, Schaffer A, Patacsil JM, Marcello AE, Glass RI, Jain V, Gentsch J. 2004. Assessment of the epidemic potential of a new strain of rotavirus associated with the novel G9 serotype which caused an outbreak in the United States for the first time in the 1995-1996 season. J Clin Microbiol 42: 1434-1438. https://doi.org/10.1128/JCM.42.4.1434-1438.2004
  5. Collins PJ, Martella V, Sleator RD, Fanning S, O'Shea H. 2010. Detection and characterisation of group A rotavirus in asymptomatic piglets in southern Ireland. Arch Virol 155: 1247-1259. https://doi.org/10.1007/s00705-010-0713-1
  6. Dormitzer PR, Nason EB, Prasad BV, Harrison SC. 2004. Structural rearrangements in the membrane penetration protein of a non-enveloped virus. Nature 430: 1053-1058. https://doi.org/10.1038/nature02836
  7. Estes MK, Palmer E, Obijeski JF. 1983. Rotaviruses: a review. Curr Top Microbiol Immunol 105: 123-184. https://doi.org/10.1007/978-3-642-69159-1_3
  8. Estes MK, Cohen J. 1989. Rotavirus gene structure and function. Microbiol Rev 53: 410-449.
  9. Estes MK, Kapikian AZ. 2007. Rotaviruses. pp. 1917-1974. In: Knipe DM, Griffin DE, Lamb RA, Straus SE, Howley PM, Martin MA, Roizman B(ed.). Fields Virology. 5th ed. Lippincott Williams & Wilkins, Philadelphia.
  10. Hoshino Y, Honma S, Jones RW, Ross J, Santos N, Gentsch JR, Kapikian AZ, Hesse RA. 2005. A porcine G9 rotavirus strain shares neutralization and VP7 phylogenetic sequence lineage 3 characteristics with contemporary human G9 rotavirus strain. Virology 332: 177-188. https://doi.org/10.1016/j.virol.2004.11.006
  11. Huang JA, Nagesha HS, Holmes IH. 1993. Comparative sequence analysis of VP4s from five Australian porcine rotaviruses: implication of an apparent new P type. Virology 196: 319-327. https://doi.org/10.1006/viro.1993.1482
  12. Jain V, Das BK, Bhan MK, Glass RI, Gentsch JR, Indian Strain Surveillance Collaborating Laboratories. 2001. Great diversity of group A rotavirus strains and high prevalence of mixed rotavirus infections in India. J Clin Microbiol 39: 3524-3529. https://doi.org/10.1128/JCM.39.10.3524-3529.2001
  13. Kang SY, Nagaraja KV, Newman JA. 1986. Primary isolation and identification of avian rotaviruses from turkeys exhibiting signs of clinical enteritis in a continuous MA104 cell line. Avian Dis 30: 494-499. https://doi.org/10.2307/1590412
  14. Kim HJ, Park SI, Ha TPM, Jeong YJ, Kim HH, Kwon HJ, Kang MI, Cho KO, Park SJ. 2010. Detection and genotyping of Korean porcine rotaviruses. Vet Microbiol 144: 274- 286. https://doi.org/10.1016/j.vetmic.2010.01.019
  15. Kirkwood CD, Palombo EA. 1997. Genetic characterization of the otavirus nonstructural protein, NSP4. Virology 236: 258-265. https://doi.org/10.1006/viro.1997.8727
  16. Lee KB, Kim JW, Lee SW, Cheon DS, Jee YM, Kang SY. 2007. Isolation and characterization of G9 human rotaviruses. J Bacteriol Virol 37: 231-240. https://doi.org/10.4167/jbv.2007.37.4.231
  17. Liprandi F, Gerder M, Bastidas Z, Lopez JA, Pujol FH, Ludert JF, Joelsson DB, Ciarlet M. 2003. A novel type of VP4 carried by a porcine rotavirus strain. Virology 315: 373-380. https://doi.org/10.1016/S0042-6822(03)00534-8
  18. Martella V, Pratelli A, Greco G, Tempesta M, Ferrari M, Losio MN, Buonavoglia C. 2001. Genomic characterization of porcine rotaviruses in Italy. Clin Diagn Lab Immunol 8: 129-132.
  19. Matthijnssens J, Ciarlet M, Rahman M, Attoui H, Bányai K, Estes MK, Gentsch JR, Iturriza-Gomara M, Kirkwood CD, Martella V, Mertens PP, Nakagomi O, Patton JT, Ruggeri FM, Saif LJ, Santos N, Steyer A, Taniguchi K, Desselberger U, Van Ranst M. 2008. Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol 153: 1621-1629. https://doi.org/10.1007/s00705-008-0155-1
  20. Monnier N, Higo-Moriguchi K, Sun ZY, Prasad BV, Taniguchi K, Dormitzer PR. 2006. High-resolution molecular and antigen structure of the VP$8^{\ast}$ core of a sialic acid-independent human rotavirus strain. J Virol 80: 1513-1523. https://doi.org/10.1128/JVI.80.3.1513-1523.2006
  21. Nakagomi T, Ohshima A, Akatani K, Ikegami N, Katsushima N, Nakagomi O. 1990. Isolation and molecular characterization of a serotype 9 human rotavirus strain. Microbiol Immunol 34: 77-82. https://doi.org/10.1111/j.1348-0421.1990.tb00994.x
  22. Okitsu S, Khamrin P, Thongprachum A, Maneekarn N, Mizuguchi M, Ushijima H. 2011. Predominance of porcine P[23] genotype rotaviruses in piglets with diarrhea in northern Thailand. J Clin Microbiol 49: 442-445. https://doi.org/10.1128/JCM.02263-10
  23. Pongsuwanna Y, Taniguchi K, Chiwakul M, Urasawa T, Wakasugi F, Jayavasu C, Urasawa S. 1996. Serological and genomic characterization of porcine rotavirus in Thailand: detection of a G10 porcine rotavirus. J Clin Microbiol 34: 1050-1057.
  24. Prasad BV, Wang GJ, Clerx JP, Chiu W. 1988. Three-dimensional structure of rotavirus. J Mol Biol 199: 269-275. https://doi.org/10.1016/0022-2836(88)90313-0
  25. Rosen BI, Parwani AV, Lopez S, Flores J, Saif LJ. 1994. Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes. J Clin Microbiol 32: 311-317.
  26. Sato K, Inaba Y, Shinozaki T, Fujii R, Matumoto M. 1981. Isolation of human rotavirus in cell cultures. Arch Virol 69: 155-160. https://doi.org/10.1007/BF01315159
  27. Shaw RD, Groene WS, Mackow ER, Merchant AA, Cheng EH. 1992. Recombinant baculovirus-expressed rotavirus protein (VP4) in an ELISPOT assay of antibody secretion. Viral Immunol 5: 51-59. https://doi.org/10.1089/vim.1992.5.51
  28. Tafazoli F, Zeng CQ, Estes MK, Magnusson KE, Svensson L. 2001. NSP4 enterotoxin of rotavirus induces paracellular leakage in polarized epithelial cells. J Virol 75: 1540- 1546. https://doi.org/10.1128/JVI.75.3.1540-1546.2001
  29. Teodoroff TA, Tsunemitsu H, Okamoto K, Katsuda K, Kohmoto M, Kawashima K, Nakagomi T, Nakagomi O. 2005. Predominance of porcine rotavirus G9 in Japanese piglets with diarrhea: close relationship of their VP7 genes with those of recent human G9 strains. J Clin Microbiol 43: 1377-1384. https://doi.org/10.1128/JCM.43.3.1377-1384.2005
  30. Yolken R, Arango-Jaramillo S, Eiden J, Vonderfecht S. 1988. Lack of genomic reassortment following infection of infant rats with group A and group B rotaviruses. J Infect Dis 158: 1120-1123. https://doi.org/10.1093/infdis/158.5.1120