Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
권호 표지
DOI QR코드

DOI QR Code

Overexpression of Periostin Protein in Non-Small Cell Lung Carcinoma is Not Related with Clinical Prognostic Significance

  • Park, Won-Young (Department of Pathology, Pusan National University Hospital, Pusan National University School of Medicine) ;
  • Shin, Dong-Hoon (Department of Pathology, Pusan National University Hospital, Pusan National University School of Medicine) ;
  • Kim, Jae-Ho (Department of Physiology, Pusan National University Hospital, Pusan National University School of Medicine) ;
  • Lee, Min-Ki (Department of Internal Medicine, Pusan National University Hospital, Pusan National University School of Medicine) ;
  • Lee, Ho-Seok (Department of Thoracic Surgery, Pusan National University Hospital, Pusan National University School of Medicine) ;
  • Lee, Chang-Hun (Department of Pathology, Pusan National University Hospital, Pusan National University School of Medicine)
  • Received : 2011.07.11
  • Accepted : 2011.10.13
  • Published : 2012.02.29
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Periostin is preferentially expressed in periosteum, indicating a potential role in bone formation. Recently, there have been emerging controversies about its role in invasion and metastasis of human malignancies. We attempted to determine the clinicopathological significance of periostin expression in non-small cell lung carcinoma (NSCLC). Methods: Immunohistochemical staining of periostin protein from 91 cases of NSCLCs was performed using tissue microarray blocks. The results were correlated with clinicopathological parameters. Results: Positive reaction to periostin was predominantly noted in the tumor stroma. The strongest reaction presented as a band-like pattern just around the tumor nests. Non-neoplastic lung tissue and most in-situ carcinomas did not show a positive reaction in their stroma. With respect to tumor differentiation, moderate to poor differentiated tumors (47/77) revealed even higher periostin expression than the well-differentiated ones (4/14) (p=0.024). High periostin expression was positively correlated with E-cadherin and p53 expression, but was not related with patient age, sex, tumor type, PCNA index, b-catenin, cyclin D1, pTNM-T, pTNM-N, stage, and patient survival (p>0.05). Conclusion: These results suggest that periostin might play a role during the biological progression of NSCLC, but may not be related to the clinical prognostic parameters.

Keywords

References

  1. Horiuchi K, Amizuka N, Takeshita S, Takamatsu H, Katsuura M, Ozawa H, et al. Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. J Bone Miner Res 1999;14:1239-49. https://doi.org/10.1359/jbmr.1999.14.7.1239
  2. Kühn B, del Monte F, Hajjar RJ, Chang YS, Lebeche D, Arab S, et al. Periostin induces proliferation of differentiated cardiomyocytes and promotes cardiac repair. Nat Med 2007;13:962-9. https://doi.org/10.1038/nm1619
  3. Lie-Venema H, Eralp I, Markwald RR, van den Akker NM, Wijffels MC, Kolditz DP, et al. Periostin expression by epicardium-derived cells is involved in the development of the atrioventricular valves and fibrous heart skeleton. Differentiation 2008;76:809-19. https://doi.org/10.1111/j.1432-0436.2007.00262.x
  4. Norris RA, Borg TK, Butcher JT, Baudino TA, Banerjee I, Markwald RR. Neonatal and adult cardiovascular pathophysiological remodeling and repair: developmental role of periostin. Ann N Y Acad Sci 2008; 1123:30-40. https://doi.org/10.1196/annals.1420.005
  5. Norris RA, Damon B, Mironov V, Kasyanov V, Ramamurthi A, Moreno-Rodriguez R, et al. Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. J Cell Biochem 2007;101:695-711. https://doi.org/10.1002/jcb.21224
  6. Takayama G, Arima K, Kanaji T, Toda S, Tanaka H, Shoji S, et al. Periostin: a novel component of subepithelial fibrosis of bronchial asthma downstream of IL-4 and IL-13 signals. J Allergy Clin Immunol 2006; 118:98-104. https://doi.org/10.1016/j.jaci.2006.02.046
  7. Bao S, Ouyang G, Bai X, Huang Z, Ma C, Liu M, et al. Periostin potently promotes metastatic growth of colon cancer by augmenting cell survival via the Akt/PKB pathway. Cancer Cell 2004;5:329-39. https://doi.org/10.1016/S1535-6108(04)00081-9
  8. Siriwardena BS, Kudo Y, Ogawa I, Kitagawa M, Kitajima S, Hatano H, et al. Periostin is frequently overexpressed and enhances invasion and angiogenesis in oral cancer. Br J Cancer 2006;95:1396-403. https://doi.org/10.1038/sj.bjc.6603431
  9. Kudo Y, Siriwardena BS, Hatano H, Ogawa I, Takata T. Periostin: novel diagnostic and therapeutic target for cancer. Histol Histopathol 2007;22:1167-74.
  10. Puglisi F, Puppin C, Pegolo E, Andreetta C, Pascoletti G, D'Aurizio F, et al. Expression of periostin in human breast cancer. J Clin Pathol 2008;61:494-8. https://doi.org/10.1136/jcp.2007.052506
  11. Kudo Y, Ogawa I, Kitajima S, Kitagawa M, Kawai H, Gaffney PM, et al. Periostin promotes invasion and anchorage- independent growth in the metastatic process of head and neck cancer. Cancer Res 2006;66:6928-35. https://doi.org/10.1158/0008-5472.CAN-05-4540
  12. Kim CJ, Yoshioka N, Tambe Y, Kushima R, Okada Y, Inoue H. Periostin is down-regulated in high grade human bladder cancers and suppresses in vitro cell invasiveness and in vivo metastasis of cancer cells. Int J Cancer 2005;117:51-8. https://doi.org/10.1002/ijc.21120
  13. Sasaki H, Lo KM, Chen LB, Auclair D, Nakashima Y, Moriyama S, et al. Expression of Periostin, homologous with an insect cell adhesion molecule, as a prognostic marker in non-small cell lung cancers. Jpn J Cancer Res 2001;92:869-73. https://doi.org/10.1111/j.1349-7006.2001.tb01174.x
  14. Sobin LH, Wittekind C. International Union against Cancer. TNM Classification of malignant tumours. 5th ed. New York: Wiley-Liss; 1997.
  15. Travis WD, Colby TV, Corrin B, Shimosato Y, Brambilla E, Sobin LH. Histological typing of lung and pleural tumours. International histological classification of tumours. 3rd ed. Berlin: Springer-Verlag; 1999.
  16. Hu M, Polyak K. Microenvironmental regulation of cancer development. Curr Opin Genet Dev 2008;18:27-34. https://doi.org/10.1016/j.gde.2007.12.006
  17. Bissell MJ, Radisky D. Putting tumours in context. Nat Rev Cancer 2001;1:46-54. https://doi.org/10.1038/35094059
  18. Radisky D, Hagios C, Bissell MJ. Tumors are unique organs defined by abnormal signaling and context. Semin Cancer Biol 2001;11:87-95. https://doi.org/10.1006/scbi.2000.0360
  19. Lopez-Maury L, Marguerat S, Bahler J. Tuning gene expression to changing environments: from rapid responses to evolutionary adaptation. Nat Rev Genet 2008;9:583-93.
  20. Takeshita S, Kikuno R, Tezuka K, Amann E. Osteoblastspecific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I. Biochem J 1993;294:271-8. https://doi.org/10.1042/bj2940271
  21. Baril P, Gangeswaran R, Mahon PC, Caulee K, Kocher HM, Harada T, et al. Periostin promotes invasiveness and resistance of pancreatic cancer cells to hypoxia-induced cell death: role of the beta4 integrin and the PI3k pathway. Oncogene 2007;26:2082-94. https://doi.org/10.1038/sj.onc.1210009
  22. Sasaki H, Dai M, Auclair D, Fukai I, Kiriyama M, Yamakawa Y, et al. Serum level of the periostin, a homologue of an insect cell adhesion molecule, as a prognostic marker in nonsmall cell lung carcinomas. Cancer 2001;92:843-8. https://doi.org/10.1002/1097-0142(20010815)92:4<843::AID-CNCR1391>3.0.CO;2-P
  23. Ouyang G, Liu M, Ruan K, Song G, Mao Y, Bao S. Upregulated expression of periostin by hypoxia in non-small-cell lung cancer cells promotes cell survival via the Akt/PKB pathway. Cancer Lett 2009;281:213-9. https://doi.org/10.1016/j.canlet.2009.02.030
  24. Hong L, Sun H, Lv X, Yang D, Zhang J, Shi Y. Expression of periostin in the serum of NSCLC and its function on proliferation and migration of human lung adenocarcinoma cell line (A549) in vitro. Mol Biol Rep 2010;37:2285-93. https://doi.org/10.1007/s11033-009-9721-1
  25. Soltermann A, Tischler V, Arbogast S, Braun J, Probst- Hensch N, Weder W, et al. Prognostic significance of epithelial-mesenchymal and mesenchymal-epithelial transition protein expression in non-small cell lung cancer. Clin Cancer Res 2008;14:7430-7. https://doi.org/10.1158/1078-0432.CCR-08-0935
  26. Shao R, Bao S, Bai X, Blanchette C, Anderson RM, Dang T, et al. Acquired expression of periostin by human breast cancers promotes tumor angiogenesis through up-regulation of vascular endothelial growth factor receptor 2 expression. Mol Cell Biol 2004;24: 3992-4003. https://doi.org/10.1128/MCB.24.9.3992-4003.2004
  27. Yan W, Shao R. Transduction of a mesenchyme- specific gene periostin into 293T cells induces cell invasive activity through epithelial-mesenchymal transformation. J Biol Chem 2006;281:19700-8. https://doi.org/10.1074/jbc.M601856200
  28. Larue L, Bellacosa A. Epithelial-mesenchymal transition in development and cancer: role of phosphatidylinositol 3' kinase/AKT pathways. Oncogene 2005;24:7443-54. https://doi.org/10.1038/sj.onc.1209091
  29. Mimeault M, Batra SK. Interplay of distinct growth factors during epithelial mesenchymal transition of cancer progenitor cells and molecular targeting as novel cancer therapies. Ann Oncol 2007;18:1605-19. https://doi.org/10.1093/annonc/mdm070
  30. Takeichi M. Cadherin cell adhesion receptors as a morphogenetic regulator. Science 1991;251:1451-5. https://doi.org/10.1126/science.2006419