DOI QR코드

DOI QR Code

마이크로 레이저 형광 여기법을 이용한 미세채널 내부에서의 산소 확산에 대한 정량적 가시화

Quantitative Visualization of Oxygen Transfer in Micro-channel using Micro-LIF Technique

  • 천쥐안 (부산대학교 일반대학원 기계공학과) ;
  • 김현동 (부산대학교 일반대학원 기계공학과) ;
  • 김경천 (부산대학교 기계공학부)
  • 투고 : 2012.03.19
  • 심사 : 2012.03.30
  • 발행 : 2012.04.30

초록

In the present study, oxygen transfer process across gas-liquid interface in a Y-shape micro-channel is quantitatively visualized using the micro laser induced fluorescence (${\mu}$-LIF) technique. Diffusion coefficient of Oxygen ($D_L$) is estimated based on the experimental results and compared to its theoretical value. Tris ruthenium (II) chloride hexahydrate was used as the oxygen quenchable fluorescent dye. A light-emitting diode (LED) with wavelength of 450 nm was used as the light source and phosphorescence images of fluorescent dye were captured by a CMOS high speed camera installed on the microscope system. Water having dissolved oxygen (DO) value of 0% and pure oxygen gas were injected into the Y-shaped microchannel by using a double loading syringe pump. In-situ pixel-by-pixel calibration was carried out to obtain Stern-Volmer plots over whole flow field. Instantaneous DO concentration fields were successfully mapped according to Stern-Volmer plots and DL was calculated as $2.0675{\times}10^{-9}\;m^2/s$.

키워드

참고문헌

  1. Garcia-Ochoa, F. and Gomez, E., 2009, "Bioreactor Scale-up and Oxygen Transfer rate in Microbial Processes: An Overview", Biotechnol. Adv., Vol. 27, pp. 153-176. https://doi.org/10.1016/j.biotechadv.2008.10.006
  2. Schapper, D., Alam, M. N. H. Z., Szita, N., Lantz, A. E. and Gernaey, K. V., 2009, "Application of Microbioreactors in Fermentation Process Development: A Review", Anal. Bioanal. Chem. Vol. 398, pp. 679-695.
  3. Philip, T. W. and, Steve, R. D., 2001, "Laser-Induced Fluorescence Studies of Oxygen Transfer Across Unsheared Flat and Wavy Air-Water Interfaces", Ind. Eng. Chem. Res., Vol. 40, pp. 1985-1995. https://doi.org/10.1021/ie000321j
  4. Dani, A., Guiraud, P. and Cockx, A., 2007, "Local Measurement of Oxygen Transfer Around a Single Bubble by Planar Laser Induced Fluorescence", Chem. Eng. Sci., Vol. 62, pp. 7245-7252. https://doi.org/10.1016/j.ces.2007.08.047
  5. Francois, N., Dietrich, N., Guiraud, P., and Cockx, A., 2011, "Direct Measurement of Mass Transfer Around a Single Bubble by Micro -PLIFI", Chem. Eng. Sci., Vol. 66 pp. 3328-3338. https://doi.org/10.1016/j.ces.2011.01.049
  6. Koo, Y. L., Cao, Y., Kopelman, R., Koo, S. M., Brasuel, M. and Philbert, M. A., 2004, "Real-time Measurements of Dissolved Oxygen Inside Live Cells by Ormosil (Organically Modi- fied Silicate) Fluorescent PEBBLE Nano- sensors", Anal. Chem., Vol. 76, pp. 2498-2505. https://doi.org/10.1021/ac035493f
  7. Song, D. H., Kim, H. D. and Kim, K. C., 2012, "Dissolved Oxygen Concentration Field Measurement in Micro-scale Water Flows using PtOEP/PS Film Sensor", Opt. Lasers Eng., Vol. 50, pp. 74-81. https://doi.org/10.1016/j.optlaseng.2011.06.026
  8. Matsuda, Y., Nagashima, F., Yamaguchi, H., Egami, Y. and Niimi, T., 2011, "Unsteady 2D Measurement of Dissolved Oxygen Distribution using Luminescent Sensor Film", Sens. Act. B, Vol. 160, pp. 1464-1467. https://doi.org/10.1016/j.snb.2011.07.031
  9. Yoon, S. Y. and Kim, K. C., 2006, "Signal Intensity Enhancement of $\mu$-LIF by using Ultra Thin Laser Sheet Illumination and Aqueous Mixture with Ethanol/methanol for Micro-channel Applications", Opt. Laser. Eng. Vol. 40(6), pp. 876-883.

피인용 문헌

  1. Visualization of oxygen distribution on leaf surfaces using VisiSens oxygen planar optode system vol.14, pp.1, 2016, https://doi.org/10.5407/JKSV.2016.14.1.051