Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Up-regulation of Thy-1 Promotes Invasion and Metastasis of Hepatocarcinomas

  • Cheng, Bian-Qiao (Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command) ;
  • Jiang, Yi (Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command) ;
  • Li, Dong-Liang (Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command) ;
  • Fan, Jing-Jing (Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command) ;
  • Ma, Ming (Department of Hepatology Center, Fuzhou General Hospital, Nanjing Military Area Command)
  • Published : 2012.04.30
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Abstract

Increasing evidence has revealed that thy-1 was a potential stem cell marker of liver cancer, but no data have been shown on how thy-1 regulates the pathophysiology of liver cancer, such as proliferation, apoptosis, invasion and migration. We previously demonstrated that thy-1 was expressed in about 1% of hepg2 cells, thy-1+hepg2 cells, but not thy-1-, demonstrating high tumorigenesis on inoculation $0.5{\times}10^5$ cells per BACA/LA mouse after 2 months. In the present study, our results showed that higher expression of thy-1 occurs in 72% (36/50 cases) of neoplastic hepatic tissues as compared to 40% (20/50 cases) of control tissues, and the expression of thy-1 is higher in poorly differentiated liver tumors than in the well-differentiated ones. In addition, thy-1 expression was detected in 85% of blood samples from liver cancer patients, but none in normal subjects or patients with cirrhosis or hepatitis. There was a significant negative correlation between thy-1expression and E-cadherin expression (a marker of invasion and migraton), but not between thy-1 expression and AFP expression in all the liver cancer and blood samples. We further investigated the relationship between thy-1 and E-cadherin in liver cancer hepg2 cell line which was transfected with pReceiver-M29/thy-1 eukaryotic expression vector followed by aspirin treatment. Lower expression of E-cadherin but higher expressions of thy-1 were detected in hepg2 cells transfected with pReceiver-M29/thy-1. Taken together, our study suggested that thy-1 probably regulates liver cancer invasion and migration.

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References

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