Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Derris scandens Benth Extract Potentiates Radioresistance of Hep-2 Laryngeal Cancer Cells

  • Hematulin, Arunee (Department of Radiation Technology, Faculty of Allied Health Science, Naresuan University) ;
  • Meethang, Sutiwan (Department of Radiation Technology, Faculty of Allied Health Science, Naresuan University) ;
  • Ingkaninan, Kornkanok (Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University) ;
  • Sagan, Daniel (Department of Radiation Technology, Faculty of Allied Health Science, Naresuan University)
  • Published : 2012.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

The use of herbal products as radiosensitizers is a promising approach to increase the efficacy of radiotherapy. However, adverse effects related to the use of herbal medicine on radiotherapy are not well characterized. The present study concerns the impact of Derris scandens Benth extract on the radiosensitivity of Hep-2 laryngeal cancer cells. Pretreatment with D. scandens extract prior to gamma irradiation significantly increased clonogenic survival and decreased the proportion of radiation-induced abnormal nuclei of Hep-2 cells. Furthermore, the extract was found to enhance radiation-induced G2/M phase arrest, induce Akt activation, and increase motility of Hep-2 cells. The study thus indicated that D. scandens extract potentiates radioresistance of Hep-2 cells, further demonstrating the importance of cellular background for the adverse effect of D. scandens extract on radiation response in a laryngeal cancer cell line.

Keywords

References

  1. Hematulin A, Ingkaninan K, Limpeanchob N, Sagan D (2011). Derris Scandens Benth Extract Radiosensitizes HT-29 Colon Cancer Cells. Siriraj Med J, 63, 85-8.
  2. Brognard J, Clark AS, Ni Y, Dennis PA (2001). Akt/protein kinase B is constitutively active in non-small cell lung cancer cells and promotes cellular survival and resistance to chemotherapy and radiation. Cancer Res, 61, 3986-97.
  3. Brownson DM, Azios N G, Fuqua BK, Dharmawardhane SF, Mabry TJ (2002). Flavonoid effects relevant to cancer. J Nutr, 132, 3482-9.
  4. Bucher N, Britten CD (2008). G2 checkpoint abrogation and checkpoint kinase-1 targeting in the treatment of cancer. Br J Cancer, 98, 523-8. https://doi.org/10.1038/sj.bjc.6604208
  5. Deckbar D, Jeggo PA, Lobrich M (2011). Understanding the limitations of radiation-induced cell cycle checkpoints. Crit Rev Biochem Mol Biol, 46, 271-83. https://doi.org/10.3109/10409238.2011.575764
  6. Girdhani S, Bhosle SM, Thulsidas SA, Kumar A, Mishra KP (2005). Potential of radiosensitizing agents in cancer chemoradiotherapy. J Cancer Res Ther, 1, 129-31. https://doi.org/10.4103/0973-1482.19585
  7. Goel A, Aggarwal BB (2010). Curcumin, the golden spice from Indian saffron, is a chemosensitizer and radiosensitizer for tumors and chemoprotector and radioprotector for normal organs. Nutr Cancer, 62, 919-30. https://doi.org/10.1080/01635581.2010.509835
  8. Habr-Gama A, Perez RO, Sao Juliao GP, Proscurshim I, Gama- Rodrigues J (2010). The need for effective radiosentitizing agents: experience in patients with complete pathological response. Anticancer Drugs, 22, 308-10.
  9. Hematulin A, Sagan D, Eckardt-Schupp F, Moertl S (2008). NBS1 is required for IGF-1 induced cellular proliferation through the Ras/Raf/MEK/ERK cascade. Cell Signal, 20, 2276-85. https://doi.org/10.1016/j.cellsig.2008.08.017
  10. Hemstrom T H, Sandstrom M, Zhivotovsky B (2006). Inhibitors of the PI3-kinase/Akt pathway induce mitotic catastrophe in non-small cell lung cancer cells. Int J Cancer, 119, 1028-38. https://doi.org/10.1002/ijc.21927
  11. Hirose Y, Katayama M, Mirzoeva OK, Berger MS, Pieper RO (2005). Akt activation suppresses Chk2-mediated, methylating agent-induced G2 arrest and protects from temozolomide-induced mitotic catastrophe and cellular senescence. Cancer Res, 65, 4861-9. https://doi.org/10.1158/0008-5472.CAN-04-2633
  12. Javvadi P, Segan AT, Tuttle SW, Koumenis C (2008). The chemopreventive agent curcumin is a potent radiosensitizer of human cervical tumor cells via increased reactive oxygen species production and overactivation of the mitogenactivated protein kinase pathway. Mol Pharmacol, 73, 1491-501. https://doi.org/10.1124/mol.107.043554
  13. Kastan MB, Bartek J (2004). Cell-cycle checkpoints and cancer. Nature, 432, 316-23. https://doi.org/10.1038/nature03097
  14. Katz D, Ito E, Liu FF (2009). On the path to seeking novel radiosensitizers. Int J Radiat Oncol Biol Phys, 73, 988-96. https://doi.org/10.1016/j.ijrobp.2008.12.002
  15. Khan S I, Zhao J, Khan IA, Walker LA, Dasmahapatra AK (2011). Potential utility of natural products as regulators of breast cancer-associated aromatase promoters. Reprod Biol Endocrinol, 9, 91. https://doi.org/10.1186/1477-7827-9-91
  16. Kim W, Seong KM, Youn B (2011). Phenylpropanoids in radioregulation: double edged sword. Exp Mol Med, 43, 323-33. https://doi.org/10.3858/emm.2011.43.6.034
  17. Kuptniratsaikul V, Pinthong T, Bunjob M, et al (2011). Efficacy and safety of Derris scandens Benth extracts in patients with knee osteoarthritis. J Altern Complement Med, 17, 147-53. https://doi.org/10.1089/acm.2010.0213
  18. Laupattarakasem P, Houghton PJ, Hoult JR (2004). Antiinflammatory isoflavonoids from the stems of Derris scandens. Planta Med, 70, 496-501. https://doi.org/10.1055/s-2004-827147
  19. Lucki NC, Sewer MB (2011). Genistein stimulates MCF-7 breast cancer cell growth by inducing acid ceramidase (ASAH1) gene expression. J Biol Chem, 286, 19399-409. https://doi.org/10.1074/jbc.M110.195826
  20. Mahabusarakam W, Deachathai S, Phongpaichit S, Jansakul C, Taylor WC (2004). A benzil and isoflavone derivatives from Derris scandens Benth. Phytochemistry, 65, 1185-91. https://doi.org/10.1016/j.phytochem.2004.03.006
  21. Maurya DK, Devasagayam TP, Nair CK (2006). Some novel approaches for radioprotection and the beneficial effect of natural products. Indian J Exp Biol, 44, 93-114.
  22. Morrison C, Rieder CL (2004). Chromosome damage and progression into and through mitosis in vertebrates. DNA Repair, 3, 1133-9. https://doi.org/10.1016/j.dnarep.2004.03.005
  23. Nambiar D, Rajamani P, Singh RP (2011). Effects of phytochemicals on ionization radiation-mediated carcinogenesis and cancer therapy. Mutat Res, 728, 139-57. https://doi.org/10.1016/j.mrrev.2011.07.005
  24. Nambiar D, Rajamani P, Singh RP (2011). Effects of phytochemicals on ionization radiation-mediated carcinogenesis and cancer therapy. Mutat Res, 728, 139-57. https://doi.org/10.1016/j.mrrev.2011.07.005
  25. Pisamai Laupattarakasem BS, Wiroon Laupattarakasem (2007). Antimigration of cancer cells by derris scandens on cholangiocarcinoma cells. Srinagarind Med J, 22, 339-45
  26. Rao SA, Srinivas PV, Tiwari AK, et al (2007). Isolation, characterization and chemobiological quantification of alpha-glucosidase enzyme inhibitory and free radical scavenging constituents from Derris scandens Benth. J Chromatogr B Analyt Technol Biomed Life Sci, 855, 166-72. https://doi.org/10.1016/j.jchromb.2007.04.048
  27. Rukachaisirikul V, Sukpondma Y, Jansakul C, Taylor WC (2002). Isoflavone glycosides from Derris scandens. Phytochemistry, 60, 827-34. https://doi.org/10.1016/S0031-9422(02)00163-2
  28. Sagar SM (2010). Can the therapeutic gain of radiotherapy be increased by concurrent administration of Asian botanicals? Integr Cancer Ther, 9, 5-13. https://doi.org/10.1177/1534735409356981
  29. Sancar A, Lindsey-Boltz LA, Unsal-Kacmaz K, Linn S (2004). Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints. Annu Rev Biochem, 73, 39-85. https://doi.org/10.1146/annurev.biochem.73.011303.073723
  30. Shin JI, Shim JH, Kim K H, et al (2008). Sensitization of the apoptotic effect of gamma-irradiation in genistein-pretreated CaSki cervical cancer cells. J Microbiol Biotechnol, 18, 523-31.
  31. Vakifahmetoglu H, Olsson M, Zhivotovsky B (2008). Death through a tragedy: mitotic catastrophe. Cell Death Differ, 15, 1153-62. https://doi.org/10.1038/cdd.2008.47
  32. Verheij M, Vens C, Van Triest B (2010). Novel therapeutics in combination with radiotherapy to improve cancer treatment: rationale, mechanisms of action and clinical perspective. Drug Resist Updat, 13, 29-43. https://doi.org/10.1016/j.drup.2010.01.002
  33. W T (1996). Dictionary of Thai medicinal plants, Bangkok: Prachumtong Printing.
  34. Zhang X, Tang N, Hadden TJ, Rishi AK (2011). Akt, FoxO and regulation of apoptosis. Biochim Biophys Acta, 1813, 1978-86. https://doi.org/10.1016/j.bbamcr.2011.03.010

Cited by

  1. Roles of glucose transporter-1 and the phosphatidylinositol 3-kinase/protein kinase B pathway in cancer radioresistance (Review) vol.11, pp.3, 2014, https://doi.org/10.3892/mmr.2014.2888
  2. Fenofibrate Increases Radiosensitivity in Head and Neck Squamous Cell Carcinoma via Inducing G2/M Arrest and Apoptosis vol.15, pp.16, 2014, https://doi.org/10.7314/APJCP.2014.15.16.6649
  3. Ethanolic Extract from Derris scandens Benth Mediates Radiosensitzation via Two Distinct Modes of Cell Death in Human Colon Cancer HT-29 Cells vol.15, pp.4, 2014, https://doi.org/10.7314/APJCP.2014.15.4.1871
  4. Radical Intermediate Generation and Cell Cycle Arrest by an Aqueous Extract of Thunbergia Laurifolia Linn. in Human Breast Cancer Cells vol.16, pp.10, 2015, https://doi.org/10.7314/APJCP.2015.16.10.4357